论文信息 - Parametric analysis of histograms measured in flow cytometry.

Parametric analysis of histograms measured in flow cytometry.

Flow cytometric histograms frequently consist of several components that show various degrees of overlap. For many types of analysis it is of great importance to decompose the original histogram into its components. To that purpose, we investigated the maximum likelihood approach in detail. It is shown that the iterative method to solve the maximum likelihood equations is well behaved for a variety of initial values. Algorithms to obtain initial values are presented, and the performance of the method is tested when applied to the analysis of DNA measurements from heterogeneous cell populations that differ with respect to DNA content.

[1] A. Sterrett,et al. Introduction to Statistical Inference. , 1963 .

[2] N. E. Day. Estimating the components of a mixture of normal distributions , 1969 .

[3] Keinosuke Fukunaga,et al. Introduction to Statistical Pattern Recognition , 1972 .

[4] J. Fried. Method for the quantitative evaluation of data from flow microfluorometry. , 1976, Computers and biomedical research, an international journal.

[5] L. S. Cram,et al. LASER FLOW MICROPHOTOMETRY FOR RAPID ANALYSIS AND SORTING OF MAMMALIAN CELLS , 1976, Annals of the New York Academy of Sciences.

[6] Mathematical evaluation of two parameter flow cytometric histograms. , 1981, Cytometry.

[7] Jack-Gérard Postaire,et al. An Approximate Solution to Normal Mixture Identification with Application to Unsupervised Pattern Classification , 1981, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[8] P. Nettesheim,et al. Changes in DNA content of rat tracheal epithelial cells during neoplastic progression in vitro. , 1982, Carcinogenesis.

[9] C Nicolini,et al. A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry , 1982, Cell and tissue kinetics.

[10] J A Steinkamp,et al. Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry. , 1982, Cytometry.