Mechanochemical synthesis evaluation of nanocrystalline bone-derived bioceramic powder using for bone tissue engineering

Introduction: Bone tissue engineering proposes a suitable way to regenerate lost bones. Different materials have been considered for use in bone tissue engineering. Hydroxyapatite (HA) is a significant success of bioceramics as a bone tissue repairing biomaterial. Among different bioceramic materials, recent interest has been risen on fluorinated hydroxyapatites, (FHA, Ca 10 (PO 4 ) 6 F x (OH) 2−x ). Fluorine ions can promote apatite formation and improve the stability of HA in the biological environments. Therefore, they have been developed for bone tissue engineering. The aim of this study was to synthesize and characterize the FHA nanopowder via mechanochemical (MC) methods. Materials and Methods: Natural hydroxyapatite (NHA) 95.7 wt.% and calcium fluoride (CaF 2 ) powder 4.3 wt.% were used for synthesis of FHA. MC reaction was performed in the planetary milling balls using a porcelain cup and alumina balls. Ratio of balls to reactant materials was 15:1 at 400 rpm rotation speed. The structures of the powdered particles formed at different milling times were evaluated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: Fabrication of FHA from natural sources like bovine bone achieved after 8 h ball milling with pure nanopowder. Conclusion: F− ion enhances the crystallization and mechanical properties of HA in formation of bone. The produced FHA was in nano-scale, and its crystal size was about 80-90 nm with sphere distribution in shape and size. FHA powder is a suitable biomaterial for bone tissue engineering.

[1]  M. Lombardi,et al.  Si-substituted hydroxyapatite nanopowders: synthesis, thermal stability and sinterability , 2009 .

[2]  K. McLean,et al.  Biological responses of human osteoblasts and osteoclasts to flame-sprayed coatings of hydroxyapatite and fluorapatite blends. , 2010, Acta biomaterialia.

[3]  M. Kharaziha,et al.  Two-step sintering of dense, nanostructural forsterite , 2009 .

[4]  Jongee Park,et al.  Photocatalytic activity of hydroxyapatite-precipitated potassium titanate whiskers , 2010 .

[5]  Ahmad Monshi,et al.  Modified Scherrer Equation to Estimate More Accurately Nano-Crystallite Size Using XRD , 2012 .

[6]  B. Nasiri-Tabrizi,et al.  Synthesis of nanosize single-crystal hydroxyapatite via mechanochemical method , 2009 .

[7]  I. Hvid,et al.  Fixation of titanium and hydroxyapatite-coated implants in arthritic osteopenic bone. , 1991, The Journal of arthroplasty.

[8]  D. Martini,et al.  Biomechanical and histomorphometric investigations on two morphologically differing titanium surfaces with and without fluorohydroxyapatite coating: an experimental study in sheep tibiae. , 2003, Biomaterials.

[9]  M. Lombardi,et al.  F-substituted hydroxyapatite nanopowders: Thermal stability, sintering behaviour and mechanical properties , 2010 .

[10]  M. Fathi,et al.  Novel fluorapatite/niobium composite coating for metallic human body implants , 2009 .

[11]  Jimin Xie,et al.  Controllable synthesis of fluorapatite nanocrystals with various morphologies: Effects of pH value and chelating reagent , 2009 .

[12]  A. Nakahira,et al.  Mechanism of incorporation of zinc into hydroxyapatite. , 2010, Acta biomaterialia.

[13]  D. Banji,et al.  FLUORIDE TOXICITY - A HARSH REALITY , 2011 .

[14]  A. Leriche,et al.  Manufacture of hydroxyapatite beads for medical applications , 2009 .

[15]  Brent Constantz,et al.  Hydroxyapatite and Related Materials , 1994 .

[16]  B. Nasiri-Tabrizi,et al.  Synthesis and characterization of fluorapatite–titania (FAp–TiO2) nanocomposite via mechanochemical process , 2010 .

[17]  M. Fathi,et al.  Mechanical alloying synthesis and bioactivity evaluation of nanocrystalline fluoridated hydroxyapatite , 2009 .

[18]  C. Bünger,et al.  Hydroxyapatite coating converts fibrous tissue to bone around loaded implants. , 1993, The Journal of bone and joint surgery. British volume.

[19]  R. Khalifehzadeh,et al.  Rapid formation of hydroxyapatite nanostrips via microwave irradiation , 2009 .

[20]  H. Hong,et al.  Cladding of titanium/fluorapatite composites onto Ti6Al4V substrate and the in vitro behaviour in the simulated body fluid , 2009 .

[21]  J. Bouaziz,et al.  Sintering of tricalcium phosphate–fluorapatite composites with zirconia , 2008 .

[22]  S. K. Sadrnezhaad,et al.  Effect of a novel sintering process on mechanical properties of hydroxyapatite ceramics , 2009 .

[23]  F. Padella,et al.  Mechanical alloying of the Fe−Zr system. Correlation between input energy and end products , 1991 .

[24]  Mingfei Jiao,et al.  Electrolytic deposition of magnesium-substituted hydroxyapatite crystals on titanium substrate , 2009 .

[25]  N. Y. Mostafa,et al.  Sintering behavior and thermal stability of Na+, SiO44― and CO32― co-substituted hydroxyapatites , 2009 .

[26]  Ebrahim Karamian,et al.  Original Research An in vitro evaluation of novel NHA/zircon plasma coating on 316L stainless steel dental implant , 2014 .

[27]  X. Miao,et al.  Effect of fluorine addition on the corrosion resistanceof hydroxyapatite ceramics , 2004 .

[28]  B. Ben-Nissan,et al.  Biological and Synthetic Apatites , 2008 .

[29]  R. Geesink,et al.  Six-year results of hydroxyapatite-coated total hip replacement. , 1995, The Journal of bone and joint surgery. British volume.

[30]  Hyoun‐Ee Kim,et al.  Stability and cellular responses to fluorapatite-collagen composites. , 2005, Biomaterials.

[31]  M. Kharaziha,et al.  Synthesis and characterization of bioactive forsterite nanopowder , 2009 .

[32]  Jian Wang,et al.  Fluoridated hydroxyapatite coatings on titanium obtained by electrochemical deposition. , 2009, Acta biomaterialia.

[33]  D. Choi,et al.  Nanostructured calcium phosphates for biomedical applications: novel synthesis and characterization. , 2005, Acta biomaterialia.

[34]  B. Bogdanov,et al.  Bioactive fluorapatite-containing glass ceramics , 2009 .