Plastid DNA polymorphism of megagametophytes and pollen in two Abies species

Single pollen grains of Abies kawakamii and megagametophytes of Abies alba and A, kawakamii were investigated for a psbC chloroplast gene region. This region was amplified and digested with endonuclease Hae III. Two different restriction fragment patterns occurred corresponding to the 2 variants detected in previous investigations on needles and embryos of Abies. The occurrence of plastid DNA in megagametophytes as well as in pollen of Abies is discussed with regard to the mechanisms of inheritance of plastid DNA in conifers. Prospects are given for PCR-based analysis of plastid DNA in single pollen grains and megagametophytes as a promising tool in population genetics.

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