Molecular Profile, Tissue Distribution and Prognostic Evaluation of a Human Melanoma-Carcinoma Antigen Recognized by the Murine Monoclonal Antibody B1.1

Using the murine monoclonal antibody (MoAb) B1.1 we have analyzed the immunochemical profile and the tissue distribution of a human melanoma associated antigen (MAA) carrying an epitope shared by the 180 kd CEA. Results of this study have demonstrated that the epitope expressed by the MAA is carried by a distinct set of molecules of 110-140 kd. Similarly to the 180 kd CEA molecules synthesized by carcinomas, the expression of the melanoma associated CEA like components (MA-CEA) is upregulated by IFN-α. The tissue distribution of MA-CEA is not restricted to malignant primary and metastatic melanocytic lesions but is found also at low levels in 64% of benign nevi. No circulating CEA was found in patients bearing widespread metastatic disease of MA-CEA positive lesions. Preliminary clinical evaluation of stage I melanoma patients bearing MoAb Bl. l positive lesions has not shown a significative prognostic association of this phenotypic marker with clinical course of the disease.

[1]  J. Lehmann,et al.  Discrimination between benign and malignant cells of melanocytic lineage by two novel antigens, a glycoprotein with a molecular weight of 113,000 and a protein with a molecular weight of 76,000. , 1987, Cancer research.

[2]  G. Mariani,et al.  Multicenter study of immunoscintigraphy with radiolabeled monoclonal antibodies in patients with melanoma. , 1986, Cancer research.

[3]  C. Taylor,et al.  Human monoclonal antibodies that distinguish cutaneous malignant melanomas from benign nevi in fixed tissue sections. , 1986, The Journal of investigative dermatology.

[4]  S. Pestka,et al.  Differential responsiveness of cloned mammary carcinoma cell populations to the human recombinant leukocyte interferon enhancement of tumor antigen expression , 1985, International journal of cancer.

[5]  D J Ruiter,et al.  Phenotypic dynamics of tumor progression in human malignant melanoma , 1985, International journal of cancer.

[6]  M. Krantz,et al.  Monoclonal antibody recognizing human melanoma-carcinoma cross-reacting oncofetal antigen epitopically associated with carcinoembryonic antigen. , 1985, Journal of the National Cancer Institute.

[7]  S. Ferrone,et al.  Distribution of human Class I (HLA-A,B,C) histocompatibility antigens in normal and malignant tissues of nonlymphoid origin. , 1984, Cancer research.

[8]  F. Veglia,et al.  Level of a membrane-bound high-molecular-weight melanoma-associated antigen and a cytoplasmic melanoma-associated antigen in surgically removed tissues and in sera from patients with melanoma. , 1984, Cancer research.

[9]  R. Rossen,et al.  Measurement of a monoclonal antibody-defined, melanoma-associated antigen in human sera: correlation of circulating antigen levels with tumor burden. , 1984, Journal of the National Cancer Institute.

[10]  A. Aguzzi,et al.  The impact of monoclonal antibodies on the study of human malignant melanoma , 1983, Journal of cutaneous pathology.

[11]  G. Steinbach,et al.  Antigenic profile of human melanoma cells. Analysis with monoclonal antibodies to histocompatibility antigens and to melanoma‐associated antigens , 1983, Journal of cutaneous pathology.

[12]  S. Leong,et al.  Mouse monoclonal antibody to a melanoma-carcinoma-associated antigen synthesized by a human melanoma cell line propagated in serum-free medium. , 1982, Cancer research.

[13]  S. Ferrone,et al.  A 94,000-dalton glycoprotein expressed by human melanoma and carcinoma cells. , 1982, Journal of the National Cancer Institute.

[14]  P. Hand,et al.  A spectrum of monoclonal antibodies reactive with human mammary tumor cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[15]  P. Noguchi,et al.  Enhancement of carcinoembryonic antigen expression by interferon , 1979, International journal of cancer.

[16]  J. Brown,et al.  Cell surface antigens of human melanoma identified by monoclonal antibody. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[17]  M. Herlyn,et al.  Study of antibodies against human melanoma produced by somatic cell hybrids. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[18]  E. Sugarbaker,et al.  Survival and regional disease control after isolation‐perfusion for invasive stage I melanoma of the extremities , 1976, Cancer.

[19]  W. Bonner,et al.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. , 1974, European journal of biochemistry.

[20]  Elisa T. Lee,et al.  A computer program for comparing K samples with right-censored data. , 1972, Computer programs in biomedicine.

[21]  S. Ferrone,et al.  Immunohistochemical detection of antigen in human primary and metastatic melanomas by the monoclonal antibody 140.240 and its possible prognostic significance , 1987, Cancer.

[22]  D. Ruiter,et al.  Class II HLA Antigens in Benign and Malignant Lesions of Melanocytic Origin , 1986 .

[23]  S. Pestka,et al.  Effects of combined treatment with interferon and mezerein on melanogenesis and growth in human melanoma cells. , 1985, Journal of interferon research.

[24]  J. Swenberg,et al.  A morphologic classification of brain tumors found in several strains of mice. , 1984, Journal of the National Cancer Institute.

[25]  P. Hand,et al.  Differential binding to human mammary and nonmammary tumors of monoclonal antibodies reactive with carcinoembryonic antigen. , 1983, Cancer Investigation.

[26]  P. Hageman,et al.  Reactions of Monoclonal Antibodies Against Human Melanoma with Different Tissues and Cell Lines , 1982 .

[27]  J. Brown,et al.  Quantitative analysis of melanoma-associated antigen p97 in normal and neoplastic tissues. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Mach,et al.  Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[29]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .