Ultra-trace determination of methylmercuy in seafood by atomic fluorescence spectrometry coupled with electrochemical cold vapor generation.

A homemade electrochemical flow cell was adopted for the determination of methylmercury. The cold vapor of mercury atoms was generated from the surface of glassycarbon cathode through the method of electrolytic reduction and detected by atomic fluorescence spectroscopy subsequently. The operating conditions were optimized with 2 ng mL(-1) methylmercury standard solution. The caliberation curve was favorably linear when the concentrations of standard HgCH3(+) solutions were in the range of 0.2-5 ng mL(-1)(as Hg). Under the optimized conditions, the limit of detection (LOD) for methylmercury was 1.88×10(-3)ng mL(-1) and the precision evaluated by relative standard deviation was 2.0% for six times 2 ng mL(-1) standard solution replicates. The terminal analytical results of seafood samples, available from local market, showed that the methylmercury content ranged within 3.7-45.8 ng g(-1). The recoveries for methylmercury spiked samples were found to be in the range of 87.6-103.6% and the relative standard deviations below 5% (n=6) were acquired, which showed this method was feasible for real sample analysis.

[1]  L. Martínez,et al.  Slurry sampling in serum blood for mercury determination by CV-AFS. , 2009, Journal of Hazardous Materials.

[2]  P. Pohl,et al.  Optimization and comparison of chemical and electrochemical hydride generation for optical emission spectrometric determination of arsenic and antimony using a novel miniaturized microwave induced argon plasma exiting the microstrip wafer. , 2008, Analytica chimica acta.

[3]  D. Das,et al.  Application of a new resin functionalised with 6-mercaptopurine for mercury and silver determination in environmental samples by atomic absorption spectrometry , 2001 .

[4]  J. F. López-Sánchez,et al.  Method development for the simultaneous determination of methylmercury and inorganic mercury in seafood , 2014 .

[5]  Farhana Zahir,et al.  Low dose mercury toxicity and human health. , 2005, Environmental toxicology and pharmacology.

[6]  M. Ganjali,et al.  Assay of Total Mercury in Commercial Food Supplements of Marine Origin by Means of DLLME/ICP-AES , 2012, Food Analytical Methods.

[7]  G. Jiang,et al.  Simple interface of high-performance liquid chromatography-atomic fluorescence spectrometry hyphenated system for speciation of mercury based on photo-induced chemical vapour generation with formic acid in mobile phase as reaction reagent. , 2008, Journal of chromatography. A.

[8]  A. Sanz-Medel,et al.  Inorganic and methylmercury speciation in environmental samples. , 1998, Talanta.

[9]  Klaus G. Heumann,et al.  Production of methylated mercury, lead, and cadmium by marine bacteria as a significant natural source for atmospheric heavy metals in polar regions , 1999 .

[10]  S. Díez,et al.  New insights into mercury bioaccumulation in deep-sea organisms from the NW Mediterranean and their human health implications. , 2013, The Science of the total environment.

[11]  F. Matysik,et al.  Optimization and evaluation of different chemical and electrochemical hydride generation systems for the determination of arsenic by microwave plasma torch optical emission spectrometry , 2004 .

[12]  J. Castillo,et al.  Determination of antimony by electrochemical hydride generation atomic absorption spectrometry in samples with high iron content using chelating resins as on-line removal system , 2006 .

[13]  Bin Chen,et al.  Methylmercury in water samples at the pg/L level by online preconcentration liquid chromatography cold vapor-atomic fluorescence spectrometry , 2015 .

[14]  A. Gholami,et al.  Determination of very low levels of dissolved mercury(II) and methylmercury in river waters by continuous flow with on-line UV decomposition and cold-vapor atomic fluorescence spectrometry after pre-concentration on a silica gel-2-mercaptobenzimidazol sorbent. , 2001, Talanta.

[15]  Yolanda Madrid Albarrán,et al.  Evaluation of different sample pre-treatment and extraction procedures for mercury speciation in fish samples , 2002 .

[16]  J. Dórea.  Exposure to Mercury and Aluminum in Early Life: Developmental Vulnerability as a Modifying Factor in Neurologic and Immunologic Effects , 2015, International journal of environmental research and public health.

[17]  H. Ebrahimzadeh,et al.  A Preconcentration Procedure for Determination of Ultra-Trace Mercury (II) in Environmental Samples Employing Continuous-Flow Cold Vapor Atomic Absorption Spectrometry , 2014, Food Analytical Methods.

[18]  Jixin Liu,et al.  Determination of mercury in gasoline by photochemical vapor generation coupled to graphite furnace atomic absorption spectrometry , 2014 .

[19]  C. Zheng,et al.  On-line preconcentration and in situ photochemical vapor generation in coiled reactor for speciation analysis of mercury and methylmercury by atomic fluorescence spectrometry , 2011 .

[20]  D. Karunasagar,et al.  Robust ultrasound assisted extraction approach using dilute TMAH solutions for the speciation of mercury in fish and plant materials by cold vapour atomic absorption spectrometry (CVAAS) , 2015 .

[21]  J. F. López-Sánchez,et al.  Inorganic mercury and methylmercury determination in polluted waters by HPLC coupled to cold vapour atomic fluorescence spectroscopy , 2012 .

[22]  J. Pavón,et al.  Mercury speciation in sea food by flow injection cold vapor atomic absorption spectrometry using selective solid phase extraction , 2008 .

[23]  N. Bings,et al.  Flow injection electrochemical hydride generation inductively coupled plasma time-of-flight mass spectrometry for the simultaneous determination of hydride forming elements and its application to the analysis of fresh water samples , 2003 .

[24]  D. Yuan,et al.  Isotopic composition analysis of dissolved mercury in seawater with purge and trap preconcentration and a modified Hg introduction device for MC-ICP-MS , 2015 .

[25]  M. Pardal,et al.  Study on bioaccumulation and biosorption of mercury by living marine macroalgae: Prospecting for a new remediation biotechnology applied to saline waters , 2015 .

[26]  A. Duarte,et al.  Simple methodology for methylmercury and inorganic mercury determinations by high-performance liquid chromatography–cold vapour atomic fluorescence spectrometry , 2001 .

[27]  R. Zamboni,et al.  Mercury speciation by liquid chromatography coupled with on-line chemical vapour generation and atomic fluorescence spectrometric detection (LC-CVGAFS). , 2005, Talanta.

[28]  A. Youssefi,et al.  Electrochemical hydride generation atomic absorption spectrometry for determination of cadmium. , 2005, Analytica chimica acta.

[29]  Qingyang Liu.  Determination of mercury and methylmercury in seafood by ion chromatography using photo-induced chemical vapor generation atomic fluorescence spectrometric detection. , 2010 .

[30]  W. Horwath,et al.  Removal of inorganic mercury and methylmercury from surface waters following coagulation of dissolved organic matter with metal-based salts. , 2011, The Science of the total environment.

[31]  Iwona Rutyna,et al.  New methodology for anodic stripping voltammetric determination of methylmercury , 2008 .

[32]  M. Vila,et al.  Optimized graphene oxide foam with enhanced performance and high selectivity for mercury removal from water. , 2016, Journal of hazardous materials.