Emission Ratiometry for Simultaneous Calcium and Action Potential Measurements with Coloaded Dyes in Rabbit Hearts: Reduction of Motion and Drift

Simultaneous Ca and AP Emission Ratiometry. Introduction: Optical measurements of the cardiac calcium transient (Ca) and transmembrane action potential (AP) may be performed simultaneously with emission ratiometry to lessen motion artifacts and photobleaching effects. We examined changes in emission spectrum in perfused rabbit hearts coloaded with Rh237 and a green‐emitting Ca dye (Fluo‐4 or Oregon Green BAPTA 1) to determine wavelength bands for emission ratiometry and to test whether ratiometry reduces motion artifacts and drift.

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