Physicochemical characteristics of pristine and functionalized graphene

Graphene‐based nanomaterials have received significant attention in the last decade due to their interesting properties. Its electrical and thermal conductivity and strength make graphene well suited for a variety of applications, particularly for use as a composite material in plastics. Furthermore, much work is taking place to utilize graphene as a biomaterial for uses such as drug delivery and tissue regeneration scaffolds. Owing to the rapid progress of graphene and its potential in many marketplaces, the potential toxicity of these materials has garnered attention. Graphene, while simple in its purest form, can have many different chemical and physical properties. In this paper, we describe our toxicity evaluation of pristine graphene and a functionalized graphene sample that has been oxidized for enhanced hydrophilicity, which was synthesized from the pristine sample. The samples were characterized by X‐ray photoelectron spectroscopy, Raman spectroscopy, infrared spectroscopy, thermogravimetric analysis, zeta‐potential, atomic force microscopy and electron microscopy. We discuss the disagreement between the size of imaged samples analyzed by atomic force microscopy and by transmission electron microscopy. Furthermore, the samples each exhibit quite different surface chemistry and structure, which directly affects their interaction with aqueous environments and is important to consider when evaluating the toxicity of materials both in vitro and in vivo. Copyright © 2017 John Wiley & Sons, Ltd.

[1]  Zhuang Liu,et al.  Nano-graphene oxide for cellular imaging and drug delivery , 2008, Nano research.

[2]  G. W. Small.  Spectrometric Identification of Organic Compounds , 1992 .

[3]  C. Fan,et al.  Protein corona-mediated mitigation of cytotoxicity of graphene oxide. , 2011, ACS nano.

[4]  D. Casciano,et al.  The role of surface chemistry in the cytotoxicity profile of graphene , 2017, Journal of applied toxicology : JAT.

[5]  Juanxia Wu,et al.  Raman spectroscopy of graphene , 2014 .

[6]  Bengt Fadeel,et al.  Classification framework for graphene-based materials. , 2014, Angewandte Chemie.

[7]  A. Balandin.  Thermal properties of graphene and nanostructured carbon materials. , 2011, Nature materials.

[8]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[9]  P. Crooks,et al.  Nanodelivery of Parthenolide Using Functionalized Nanographene Enhances its Anticancer Activity. , 2015, RSC advances.

[10]  N. Chatterjee,et al.  Screening of toxic potential of graphene family nanomaterials using in vitro and alternative in vivo toxicity testing systems , 2015, Environmental health and toxicology.

[11]  A. Bhatt,et al.  Stabilizing lithium metal using ionic liquids for long-lived batteries , 2016, Nature Communications.

[12]  Suck Won Hong,et al.  Cytotoxicity evaluations of pristine graphene and carbon nanotubes in fibroblastic cells , 2012 .

[13]  Christy L Haynes,et al.  Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. , 2011, ACS applied materials & interfaces.

[14]  Yi Jia,et al.  Graphene‐On‐Silicon Schottky Junction Solar Cells , 2010, Advanced materials.

[15]  Keryn Lian,et al.  Graphene-based Electrodes , 2013 .

[16]  E. Borowiak‐Palen,et al.  Synthesis, dispersion, and cytocompatibility of graphene oxide and reduced graphene oxide. , 2012, Colloids and surfaces. B, Biointerfaces.

[17]  R. Zhou,et al.  The role of basic residues in the adsorption of blood proteins onto the graphene surface , 2015, Scientific Reports.

[18]  M. Pumera,et al.  The toxicity of graphene oxides: dependence on the oxidative methods used. , 2013, Chemistry.

[19]  P. Kim,et al.  Experimental observation of the quantum Hall effect and Berry's phase in graphene , 2005, Nature.

[20]  L. Amelse,et al.  Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering , 2015, Journal of applied toxicology : JAT.

[21]  John Parthenios,et al.  Chemical oxidation of multiwalled carbon nanotubes , 2008 .

[22]  J. Kysar,et al.  Measurement of the Elastic Properties and Intrinsic Strength of Monolayer Graphene , 2008, Science.

[23]  C. Tung,et al.  Inside Cover: Graphene‐Supported Ultrafine Metal Nanoparticles Encapsulated by Mesoporous Silica: Robust Catalysts for Oxidation and Reduction Reactions (Angew. Chem. Int. Ed. 1/2014) , 2014 .

[24]  R. Ruoff,et al.  Graphene-based ultracapacitors. , 2008, Nano letters.

[25]  M. Schwab,et al.  Inkjet-printed energy storage device using graphene/polyaniline inks , 2014 .

[26]  J. Robertson,et al.  Interpretation of Raman spectra of disordered and amorphous carbon , 2000 .

[27]  M. M. Lucchese,et al.  Evolution of the Raman spectra from single-, few-, and many-layer graphene with increasing disorder , 2010 .

[28]  F. Guinea,et al.  The electronic properties of graphene , 2007, Reviews of Modern Physics.

[29]  Bing Yan,et al.  Size-dependent cell uptake of protein-coated graphene oxide nanosheets. , 2012, ACS applied materials & interfaces.

[30]  S. Stankovich,et al.  Chemical analysis of graphene oxide films after heat and chemical treatments by X-ray photoelectron and Micro-Raman spectroscopy , 2009 .

[31]  Lin Zhao,et al.  Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane. , 2015, Nanoscale.

[32]  G. Salamo,et al.  Adhesive force between graphene nanoscale flakes and living biological cells , 2017, Journal of applied toxicology : JAT.

[33]  Hye Rim Cho,et al.  A graphene-based electrochemical device with thermoresponsive microneedles for diabetes monitoring and therapy. , 2016, Nature nanotechnology.

[34]  D. Bhattacharyya,et al.  Large-area graphene-based nanofiltration membranes by shear alignment of discotic nematic liquid crystals of graphene oxide , 2016, Nature Communications.