The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells.

Abstract Certain clonal teratocarcinoma stem cell lines can be maintained in the undifferentiated state or can be stimulated to differentiate by a simple alteration in the culture conditions. In these cultures, differentiation occurs in a clearly definable, relatively synchronous sequence of events, as the entire cell population participates in the process of “cystic embryoid body” formation. We have studied the morphological changes that occur during this process. The pattern of development in the embryoid bodies is remarkably similar to that in normal mouse embryogenesis up to the time of formation of the third germ layer, the mesoderm. Whereas, in some embryoid bodies, mesoderm appears to form in a relatively normal manner, in most it apparently arises by a process which has not previously been described. These results are discussed in comparison with similar studies on the development of isolated mouse inner cell masses in vitro .

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