Preparation and Control of Ethylene Glycol-Stabilized Haemolysates for Glycated Haemoglobin Assay

The preparation and evaluation of ethylene glycol-stabilized haemolysates for use as control material for the assay of glycated haemoglobins is described. These haemolysates were prepared from normal and diabetic blood samples by following the procedure normally used to purify human haemoglobin, with the addition of dialysis to remove glucose from the labile fractions, and dilution with ethylene glycol. All the haemoglobin fractions were converted into the carbon monoxide form to increase their stability and were stored under different conditions. During a 10 month period of storage at -20 degrees C no significant change in the glycated haemoglobins level was observed.

[1]  D. Goldstein.  Is glycosylated hemoglobin clinically useful? , 1984, The New England journal of medicine.

[2]  C. Franzini.  Ethylene glycol-stabilized haemolysates as control material in haemoglobinometry. , 1983, Clinica chimica acta; international journal of clinical chemistry.

[3]  D. Goldstein,et al.  Glycosylated hemoglobin measured by affinity chromatography: micro-sample collection and room-temperature storage. , 1983, Clinical chemistry.

[4]  J. Burrin,et al.  A collaborative study of the measurement of glycosylated haemoglobin by several methods in seven laboratories in the United Kingdom , 1983, Diabetologia.

[5]  D. Nathan,et al.  Rapid method for eliminating labile glycosylated hemoglobin from the assay for hemoglobin A1. , 1982, Clinical chemistry.

[6]  P. Basset,et al.  An update on electrophoretic and chromatographic methods in the diagnosis of hemoglobinopathies. , 1982, Journal of chromatography.

[7]  R. Kawahara,et al.  Glycosylated Hemoglobin in Relation to Rapid Fluctuations in Blood Glucose in Children with Insulin-dependent Diabetes: A Comparison of Methods With and Without Prior Dialysis , 1981, Diabetes Care.

[8]  K. Gekko,et al.  Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures. , 1981, Biochemistry.

[9]  R. Barnett,et al.  Long-term stability of a stabilized liquid quality-control serum. , 1981, Clinical chemistry.

[10]  L. Carey,et al.  Concentration of free fatty acids in pancreatitis-associated ascitic fluid. , 1981, Clinical chemistry.

[11]  R Shapiro,et al.  Sites of nonenzymatic glycosylation of human hemoglobin A. , 1980, The Journal of biological chemistry.

[12]  A. Cerami,et al.  Correlation of glucose regulation and hemoglobin AIc in diabetes mellitus. , 1976, The New England journal of medicine.