Recent trends in natural polysaccharide based bioinks for multiscale 3D printing in tissue regeneration: A review.

Biofabrication by three-dimensional (3D) printing has been an attractive technology in harnessing the possibility to print anatomical shaped native tissues with controlled architecture and resolution. 3D printing offers the possibility to reproduce complex microarchitecture of native tissues by printing live cells in a layer by layer deposition to provide a biomimetic structural environment for tissue formation and host tissue integration. Plant based biomaterials derived from green and sustainable sources have represented to emulate native physicochemical and biological cues in order to direct specific cellular response and formation of new tissues through biomolecular recognition patterns. This comprehensive review aims to analyze and identify the most commonly used plant based bioinks for 3D printing applications. An overview on the role of different plant based biomaterial of terrestrial origin (Starch, Nanocellulose and Pectin) and marine origin (Ulvan, Alginate, Fucoidan, Agarose and Carrageenan) used for 3D printing applications are discussed elaborately. Furthermore, this review will also emphasis in the functional aspects of different 3D printers, appropriate printing material, merits and demerits of numerous plant based bioinks in developing 3D printed tissue-like constructs. Additionally, the underlying potential benefits, limitations and future perspectives of plant based bioinks for tissue engineering (TE) applications are also discussed.

[1]  Anja Lode,et al.  Three‐dimensional plotting of a cell‐laden alginate/methylcellulose blend: towards biofabrication of tissue engineering constructs with clinically relevant dimensions , 2017, Journal of tissue engineering and regenerative medicine.

[2]  Yu Jun Tan,et al.  A strategy for strong interface bonding by 3D bioprinting of oppositely charged κ-carrageenan and gelatin hydrogels. , 2018, Carbohydrate polymers.

[3]  M. Tako,et al.  Rheological Characteristics of Fucoidan Isolated from Commercially Cultured Cladosiphon okamuranus , 2003 .

[4]  G. Wallace,et al.  On Low-Concentration Inks Formulated by Nanocellulose Assisted with Gelatin Methacrylate (GelMA) for 3D Printing toward Wound Healing Application , 2019, ACS applied materials & interfaces.

[5]  V. Guillard,et al.  Vegetal fiber‐based biocomposites: Which stakes for food packaging applications? , 2016 .

[6]  Feng Zhou,et al.  Direct ink writing with high-strength and swelling-resistant biocompatible physically crosslinked hydrogels. , 2019, Biomaterials science.

[7]  Z. Jessop,et al.  Printability of pulp derived crystal, fibril and blend nanocellulose-alginate bioinks for extrusion 3D bioprinting , 2019, Biofabrication.

[8]  A. Feinberg,et al.  Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues. , 2019, Materials today. Chemistry.

[9]  Alain Dufresne,et al.  Nanocellulose in biomedicine: Current status and future prospect , 2014 .

[10]  Horst Fischer,et al.  A tailored three-dimensionally printable agarose–collagen blend allows encapsulation, spreading, and attachment of human umbilical artery smooth muscle cells , 2016, Biofabrication.

[11]  Pedro L Granja,et al.  Cell-instructive pectin hydrogels crosslinked via thiol-norbornene photo-click chemistry for skin tissue engineering. , 2018, Acta biomaterialia.

[12]  Peter Enoksson,et al.  Tailor-made conductive inks from cellulose nanofibrils for 3D printing of neural guidelines. , 2018, Carbohydrate polymers.

[13]  Paolo De Coppi,et al.  Opportunities and challenges of translational 3D bioprinting , 2019, Nature Biomedical Engineering.

[14]  Dong-Woo Cho,et al.  An additive manufacturing‐based PCL–alginate–chondrocyte bioprinted scaffold for cartilage tissue engineering , 2015, Journal of tissue engineering and regenerative medicine.

[15]  John W Haycock,et al.  3D cell culture: a review of current approaches and techniques. , 2011, Methods in molecular biology.

[16]  W. Świȩszkowski,et al.  PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration. , 2017, Biofabrication.

[17]  Min Suk Shim,et al.  Preparation and characterization of chitosan-natural nano hydroxyapatite-fucoidan nanocomposites for bone tissue engineering. , 2016, International journal of biological macromolecules.

[18]  Yongxiang Luo,et al.  Cell-laden four-dimensional bioprinting using near-infrared-triggered shape-morphing alginate/polydopamine bioinks , 2019, Biofabrication.

[19]  Hyeongjin Lee,et al.  Fabrication of hASCs-laden structures using extrusion-based cell printing supplemented with an electric field. , 2016, Acta biomaterialia.

[20]  Ø. Arlov,et al.  Exploitation of Cationic Silica Nanoparticles for Bioprinting of Large-Scale Constructs with High Printing Fidelity. , 2018, ACS applied materials & interfaces.

[21]  F. Chiellini,et al.  Ulvan as a New Type of Biomaterial from Renewable Resources: Functionalization and Hydrogel Preparation , 2010 .

[22]  J. S. Colombo,et al.  Synthesis and characterization of novel calcium phosphate glass-derived cements for vital pulp therapy , 2020, Journal of Materials Science: Materials in Medicine.

[23]  W. Świȩszkowski,et al.  PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration , 2017, Biofabrication.

[24]  H. Domínguez,et al.  In vitro antioxidant properties of crude extracts and compounds from brown algae. , 2013, Food chemistry.

[25]  M. Rudert,et al.  Antibiotic elution and compressive strength of gentamicin/vancomycin loaded bone cements are considerably influenced by immersion fluid volume , 2019, Journal of Materials Science: Materials in Medicine.

[26]  Costas Fotakis,et al.  Femtosecond laser printing of living cells using absorbing film-assisted laser-induced forward transfer , 2012 .

[27]  F. O'Brien.  Biomaterials & scaffolds for tissue engineering , 2011 .

[28]  Ying Mei,et al.  Engineering alginate as bioink for bioprinting. , 2014, Acta biomaterialia.

[29]  Lay Poh Tan,et al.  Current Status of Bioinks for Micro-Extrusion-Based 3D Bioprinting , 2016, Molecules.

[30]  G. Vozzi,et al.  Pectin-GPTMS based biomaterial: toward a sustainable Bioprinting of 3D scaffolds for Tissue Engineering application. , 2019, Biomacromolecules.

[31]  T. Zvyagintseva,et al.  Anticoagulant Activity of Fucoidan from Brown Algae Fucus evanescens of the Okhotsk Sea , 2003, Bulletin of Experimental Biology and Medicine.

[32]  Thomas H. Jovic,et al.  Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications , 2019, Front. Mech. Eng..

[33]  Xingyi Huang,et al.  Effect of ultrasonic treatment on the degradation and inhibition cancer cell lines of polysaccharides from Porphyra yezoensis. , 2015, Carbohydrate polymers.

[34]  Chee Kai Chua,et al.  Indirect fabrication of collagen scaffold based on inkjet printing technique , 2006 .

[35]  Murielle Maire,et al.  Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum , 2016, Marine drugs.

[36]  Shan Huang,et al.  Enzymatically degradable alginate/gelatin bioink promotes cellular behavior and degradation in vitro and in vivo , 2019, Biofabrication.

[37]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[38]  M. Murali,et al.  Incorporation of Fucoidan in β-Tricalcium phosphate-Chitosan scaffold prompts the differentiation of human bone marrow stromal cells into osteogenic lineage , 2016, Scientific Reports.

[39]  N. Dutta,et al.  3D Bioprinted Nanocellulose-Based Hydrogels for Tissue Engineering Applications: A Brief Review , 2019, Polymers.

[40]  Andreas Kurtz,et al.  Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies , 2018, International journal of molecular sciences.

[41]  S. Fedorov,et al.  Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects , 2013, Marine drugs.

[42]  P. Gatenholm,et al.  Alginate Sulfate–Nanocellulose Bioinks for Cartilage Bioprinting Applications , 2016, Annals of Biomedical Engineering.

[43]  Peter Pivonka,et al.  In situ handheld three‐dimensional bioprinting for cartilage regeneration , 2018, Journal of tissue engineering and regenerative medicine.

[44]  A. Khademhosseini,et al.  Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low‐Viscosity Bioink , 2016, Advanced materials.

[45]  Ira Bhatnagar,et al.  Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering , 2014, Marine drugs.

[46]  Ibrahim T. Ozbolat,et al.  Current advances and future perspectives in extrusion-based bioprinting. , 2016, Biomaterials.

[47]  Qing Gao,et al.  Research on the printability of hydrogels in 3D bioprinting , 2016, Scientific Reports.

[48]  R. Reis,et al.  Marine algae sulfated polysaccharides for tissue engineering and drug delivery approaches , 2012, Biomatter.

[49]  Jae Young Lee,et al.  Three dimensional cell printing with sulfated alginate for improved bone morphogenetic protein-2 delivery and osteogenesis in bone tissue engineering. , 2018, Carbohydrate polymers.

[50]  S. Kikionis,et al.  Hybrid Sponge-Like Scaffolds Based on Ulvan and Gelatin: Design, Characterization and Evaluation of Their Potential Use in Bone Tissue Engineering , 2020, Materials.

[51]  F. Auricchio,et al.  Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue , 2019, Cells.

[52]  Hermann Seitz,et al.  Biomaterials as Scaffold for Bone Tissue Engineering , 2006, European Journal of Trauma.

[53]  Jenneke Klein-Nulend,et al.  The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method , 2018, Journal of functional biomaterials.

[54]  H. Yun,et al.  A simultaneous 3D printing process for the fabrication of bioceramic and cell-laden hydrogel core/shell scaffolds with potential application in bone tissue regeneration. , 2016, Journal of materials chemistry. B.

[55]  S. Ramtani,et al.  The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering , 2019, Polymers.

[56]  Rossana Schipani,et al.  Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering , 2019, Advanced healthcare materials.

[57]  Angela Faustino Jozala,et al.  Bacterial nanocellulose production and application: a 10-year overview , 2015, Applied Microbiology and Biotechnology.

[58]  G. Ateshian,et al.  Scaffold degradation elevates the collagen content and dynamic compressive modulus in engineered articular cartilage. , 2009, Osteoarthritis and cartilage.

[59]  F. Guillemot,et al.  Effect of laser energy, substrate film thickness and bioink viscosity on viability of endothelial cells printed by Laser-Assisted Bioprinting , 2011 .

[60]  Hon Fai Chan,et al.  3D Printing of Highly Stretchable and Tough Hydrogels into Complex, Cellularized Structures , 2015, Advanced materials.

[61]  B. Kulseng,et al.  Transplantation of alginate microcapsules: generation of antibodies against alginates and encapsulated porcine islet-like cell clusters. , 1999, Transplantation.

[62]  B. Frank Eames,et al.  Application of Extrusion-Based Hydrogel Bioprinting for Cartilage Tissue Engineering , 2017, International journal of molecular sciences.

[63]  Serge Pérez,et al.  The molecular structures of starch components and their contribution to the architecture of starch granules: A comprehensive review , 2010 .

[64]  Gordon G Wallace,et al.  Functional 3D Neural Mini‐Tissues from Printed Gel‐Based Bioink and Human Neural Stem Cells , 2016, Advanced healthcare materials.

[65]  Denis Cormier,et al.  Bio-printing cell-laden Matrigel–agarose constructs , 2016, Journal of biomaterials applications.

[66]  Ashlie Martini,et al.  Cellulose nanomaterials review: structure, properties and nanocomposites. , 2011, Chemical Society reviews.

[67]  Xiaohong Wang,et al.  Rapid prototyping as a tool for manufacturing bioartificial livers. , 2007, Trends in biotechnology.

[68]  Rajender S. Varma,et al.  Plants and plant-based polymers as scaffolds for tissue engineering , 2019, Green Chemistry.

[69]  Ali Khademhosseini,et al.  Bioinks for 3D bioprinting: an overview. , 2018, Biomaterials science.

[70]  Minna Kellomäki,et al.  A review of rapid prototyping techniques for tissue engineering purposes , 2008, Annals of medicine.

[71]  Lan Li,et al.  Evaluation of a polyvinyl alcohol-alginate based hydrogel for precise 3D bioprinting. , 2018, Journal of biomedical materials research. Part A.

[72]  Won-Kyo Jung,et al.  Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting. , 2019, Journal of the mechanical behavior of biomedical materials.

[73]  Anthony Atala,et al.  Optimization of gelatin–alginate composite bioink printability using rheological parameters: a systematic approach , 2018, Biofabrication.

[74]  R. Reis,et al.  Processing ulvan into 2D structures: cross-linked ulvan membranes as new biomaterials for drug delivery applications. , 2012, International journal of pharmaceutics.

[75]  F. Sepulcre,et al.  Assessing Mechanical and Rheological Properties of Potato Puree: Effect of Different Ingredient Combinations and Cooking Methods on the Feasibility of 3D Printing , 2019, Foods.

[76]  Adrian Neagu,et al.  Tissue engineering by self-assembly of cells printed into topologically defined structures. , 2008, Tissue engineering. Part A.

[77]  B. Salehi,et al.  Therapeutic Potential of Alpha- and Beta-Pinene A Miracle Gift of Nature , 2019 .

[78]  Jesse K. Placone,et al.  Addressing present pitfalls in 3D printing for tissue engineering to enhance future potential , 2020, APL bioengineering.

[79]  Xiaodong Xu,et al.  3D printing process of oxidized nanocellulose and gelatin scaffold , 2018, Journal of biomaterials science. Polymer edition.

[80]  Nupura S. Bhise,et al.  Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels , 2014, Biofabrication.

[81]  Ana Grenha,et al.  Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications , 2016, Marine drugs.

[82]  P. Dubruel,et al.  Biofunctionalization of ulvan scaffolds for bone tissue engineering. , 2014, ACS applied materials & interfaces.

[83]  A. Perriman,et al.  3D Bioprinting Using a Templated Porous Bioink , 2016, Advanced healthcare materials.

[84]  P. Dubruel,et al.  Ulvan-chitosan polyelectrolyte complexes as matrices for enzyme induced biomimetic mineralization. , 2018, Carbohydrate polymers.

[85]  J. Sassi,et al.  Ultrastructure of ulvan: A polysaccharide from green seaweeds , 2009, Biopolymers.

[86]  D. French.  Fine Structure of Starch and its Relationship to the Organization of Starch Granules , 1972 .

[87]  D. Kelly,et al.  A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage , 2016, Biofabrication.

[88]  Aleksandra M Urbanska,et al.  Agarose-based biomaterials for tissue engineering. , 2018, Carbohydrate polymers.

[89]  Wei Sun,et al.  Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells , 2016, Biofabrication.

[90]  Thomas J Webster,et al.  Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications , 2019, Biomolecules.

[91]  Jae Young Lee,et al.  Graphene oxide/alginate composites as novel bioinks for three-dimensional mesenchymal stem cell printing and bone regeneration applications. , 2019, Nanoscale.

[92]  Wei Sun,et al.  The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology , 2015, Biofabrication.

[93]  Wei Sun,et al.  Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation , 2016, Scientific Reports.

[94]  Daniel J. Modulevsky,et al.  Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture , 2014, PloS one.

[95]  Dong-Woo Cho,et al.  Development of a 3D cell printed structure as an alternative to autologs cartilage for auricular reconstruction. , 2017, Journal of biomedical materials research. Part B, Applied biomaterials.

[96]  R. Reis,et al.  Engineering bioinks for 3D bioprinting , 2021, Biofabrication.

[97]  Eben Alsberg,et al.  * Three-Dimensional Bioprinting of Polycaprolactone Reinforced Gene Activated Bioinks for Bone Tissue Engineering. , 2017, Tissue engineering. Part A.

[98]  P. Gatenholm,et al.  Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink , 2017, Scientific Reports.

[99]  F. Bastami,et al.  3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering. , 2017, Dental materials : official publication of the Academy of Dental Materials.

[100]  Juan de Vicente,et al.  Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs. , 2020, Acta biomaterialia.

[101]  Jessica D. Kaufman,et al.  A novel biocompatible adhesive incorporating plant-derived monomers. , 2009, Journal of biomedical materials research. Part A.

[102]  Paul Gatenholm,et al.  Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds , 2017, Biofabrication.

[103]  Junhong Chen,et al.  Novel bioprinting method using a pectin based bioink. , 2017, Technology and health care : official journal of the European Society for Engineering and Medicine.

[104]  Carmen C Piras,et al.  Multicomponent polysaccharide alginate-based bioinks. , 2020, Journal of materials chemistry. B.

[105]  A. Lode,et al.  3D Bioprinting of osteochondral tissue substitutes – in vitro-chondrogenesis in multi-layered mineralized constructs , 2020, Scientific Reports.

[106]  J. Massera,et al.  Wood-based nanocellulose and bioactive glass modified gelatin–alginate bioinks for 3D bioprinting of bone cells , 2019, Biofabrication.

[107]  Hyeongjin Lee,et al.  Mechanically reinforced cell-laden scaffolds formed using alginate-based bioink printed onto the surface of a PCL/alginate mesh structure for regeneration of hard tissue. , 2016, Journal of colloid and interface science.

[108]  I. Stancu,et al.  Bioinspired 3D printable pectin-nanocellulose ink formulations. , 2019, Carbohydrate polymers.

[109]  Liying Li,et al.  Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs. , 2019, International journal of biological macromolecules.

[110]  Bethany C Gross,et al.  Evaluation of 3D printing and its potential impact on biotechnology and the chemical sciences. , 2014, Analytical chemistry.

[111]  Andreas Mark,et al.  Simulations of 3D bioprinting: predicting bioprintability of nanofibrillar inks , 2018, Biofabrication.

[112]  Thomas Schmitz-Rode,et al.  Three-Dimensional Printing and Angiogenesis: Tailored Agarose-Type I Collagen Blends Comprise Three-Dimensional Printability and Angiogenesis Potential for Tissue-Engineered Substitutes. , 2017, Tissue engineering. Part C, Methods.

[113]  W. Suwanpreuk,et al.  Fabrication of bioactive hydroxyapatite/bis-GMA based composite via three dimensional printing , 2008, Journal of materials science. Materials in medicine.

[114]  Oliver Friedrich,et al.  Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication , 2019, Front. Bioeng. Biotechnol..

[115]  T Ahlfeld,et al.  Development of a clay based bioink for 3D cell printing for skeletal application , 2017, Biofabrication.

[116]  Swati Midha,et al.  Advances in three‐dimensional bioprinting of bone: Progress and challenges , 2019, Journal of tissue engineering and regenerative medicine.

[117]  Chien‐Ho Chen,et al.  Development of Injectable Fucoidan and Biological Macromolecules Hybrid Hydrogels for Intra-Articular Delivery of Platelet-Rich Plasma , 2019, Marine drugs.

[118]  A. Lode,et al.  Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink , 2018, Biofabrication.

[119]  G Cidonio,et al.  The cell in the ink: Improving biofabrication by printing stem cells for skeletal regenerative medicine , 2019, Biomaterials.

[120]  Nhayoung Hong,et al.  3D bioprinting and its in vivo applications. , 2018, Journal of biomedical materials research. Part B, Applied biomaterials.

[121]  D. Fatouros,et al.  Development of Bio-Active Patches Based on Pectin for the Treatment of Ulcers and Wounds Using 3D-Bioprinting Technology , 2020, Pharmaceutics.

[122]  A. Bandyopadhyay,et al.  Bone tissue engineering using 3D printing , 2013 .

[123]  W. Dhert,et al.  Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. , 2008, Tissue engineering. Part A.

[124]  FischerHorst,et al.  The stiffness and structure of three-dimensional printed hydrogels direct the differentiation of mesenchymal stromal cells toward adipogenic and osteogenic lineages. , 2015 .

[125]  Elsa Pastor,et al.  A Data-Driven Fire Spread Simulator: Validation in Vall-llobrega's Fire , 2019, Front. Mech. Eng..

[126]  Ahu Gumrah Dumanli,et al.  Nanocellulose and its Composites for Biomedical Applications. , 2017, Current medicinal chemistry.

[127]  P. Bártolo,et al.  Additive manufacturing of tissues and organs , 2012 .

[128]  Ralf Smeets,et al.  An Introduction to 3D Bioprinting: Possibilities, Challenges and Future Aspects , 2018, Materials.

[129]  Xingchen Yang,et al.  Collagen-alginate as bioink for three-dimensional (3D) cell printing based cartilage tissue engineering. , 2018, Materials science & engineering. C, Materials for biological applications.

[130]  D. Kelly,et al.  Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues , 2017, Scientific Reports.

[131]  Nicolas H Voelcker,et al.  Mechanically Tunable Bioink for 3D Bioprinting of Human Cells , 2017, Advanced healthcare materials.

[132]  B. Yao,et al.  Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and differentiation. , 2019, International journal of biological macromolecules.

[133]  A. Lode,et al.  Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes , 2019, Journal of Materials Science: Materials in Medicine.

[134]  Gianluca Fontana,et al.  Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds. , 2017, Biomaterials.

[135]  D. Mooney,et al.  Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution. , 2004, Biomacromolecules.

[136]  H. Fischer,et al.  Three-dimensional printing of stem cell-laden hydrogels submerged in a hydrophobic high-density fluid , 2012, Biofabrication.

[137]  J. Malda,et al.  Printability and Shape Fidelity of Bioinks in 3D Bioprinting , 2020, Chemical reviews.

[138]  Eneko Axpe,et al.  Applications of Alginate-Based Bioinks in 3D Bioprinting , 2016, International journal of molecular sciences.

[139]  M. Magnusson,et al.  Ulvan: A systematic review of extraction, composition and function , 2019, Algal Research.

[140]  Guang Yang,et al.  Bioprinting and its applications in tissue engineering and regenerative medicine. , 2018, International journal of biological macromolecules.

[141]  H. Tønnesen,et al.  Alginate in Drug Delivery Systems , 2002, Drug development and industrial pharmacy.

[142]  G. Chinga-Carrasco.  Potential and Limitations of Nanocelluloses as Components in Biocomposite Inks for Three-Dimensional Bioprinting and for Biomedical Devices. , 2018, Biomacromolecules.

[143]  D. Manners.  Recent developments in our understanding of amylopectin structure , 1989 .

[144]  Jean Martínez,et al.  Chemical insights into bioinks for 3D printing. , 2019, Chemical Society reviews.

[145]  M. Perán,et al.  Volume-by-volume bioprinting of chondrocytes-alginate bioinks in high temperature thermoplastic scaffolds for cartilage regeneration , 2019, Experimental biology and medicine.

[146]  Keekyoung Kim,et al.  3D bioprinting for engineering complex tissues. , 2016, Biotechnology advances.

[147]  I. Michalak,et al.  Methods of extraction, physicochemical properties of alginates and their applications in biomedical field – a review , 2019, Open Chemistry.

[148]  Yi‐Cheng Huang,et al.  Chitosan/Fucoidan pH Sensitive Nanoparticles for Oral Delivery System , 2011 .

[149]  Weng-Sing Hwang,et al.  Direct Printing of 1-D and 2-D Electronically Conductive Structures by Molten Lead-Free Solder , 2016, Materials.

[150]  Se-kwon Kim,et al.  The Suppressive Activity of Fucofuroeckol-A Derived from Brown Algal Ecklonia stolonifera Okamura on UVB-Induced Mast Cell Degranulation , 2018, Marine drugs.

[151]  R. Landers,et al.  Biofunctional rapid prototyping for tissue‐engineering applications: 3D bioplotting versus 3D printing , 2004 .

[152]  G. Wallace,et al.  3D printing of nanocellulose hydrogel scaffolds with tunable mechanical strength towards wound healing application. , 2018, Journal of materials chemistry. B.

[153]  M. Österberg,et al.  Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing , 2019, ACS applied materials & interfaces.

[154]  Horst Fischer,et al.  Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering , 2016, Advanced healthcare materials.

[155]  Ibrahim T. Ozbolat,et al.  Bioprinting Technology: A Current State-of-the-Art Review , 2014 .

[156]  M. Gümüşderelioğlu,et al.  A bioprintable form of chitosan hydrogel for bone tissue engineering , 2017, Biofabrication.

[157]  Ying Mei,et al.  3D Bioprinting for Vascularized Tissue Fabrication , 2016, Annals of Biomedical Engineering.

[158]  V. Pillay,et al.  A 3D bioprinted in situ conjugated-co-fabricated scaffold for potential bone tissue engineering applications. , 2018, Journal of Biomedical Materials Research. Part A.

[159]  I. Noh,et al.  Recent trends in bioinks for 3D printing , 2018, Biomaterials Research.

[160]  D. Mooney,et al.  Alginate: properties and biomedical applications. , 2012, Progress in polymer science.

[161]  L. Roshangar,et al.  An Experimental Study on the Mechanical and Biological Properties of Bio-Printed Alginate/Halloysite Nanotube/Methylcellulose/Russian Olive-Based Scaffolds , 2018, Advanced pharmaceutical bulletin.

[162]  I. Banerjee,et al.  Biomineralization of Fucoidan-Peptide Blends and Their Potential Applications in Bone Tissue Regeneration , 2017, Journal of functional biomaterials.

[163]  Rainer Schmelzeisen,et al.  Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques , 2002 .

[164]  Rui L. Reis,et al.  Alternative tissue engineering scaffolds based on starch: processing methodologies, morphology, degradation and mechanical properties , 2002 .

[165]  Chunlin Xu,et al.  Nanocellulose-Based Inks for 3D Bioprinting: Key Aspects in Research Development and Challenging Perspectives in Applications—A Mini Review , 2020, Bioengineering.

[166]  L. Bertassoni,et al.  A Dentin-derived hydrogel bioink for 3D printing of cell laden scaffolds for regenerative dentistry. , 2017, Biofabrication.

[167]  Ali Khademhosseini,et al.  Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. , 2016, Biomaterials.

[168]  Jianhua Zhang,et al.  Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology , 2019, Biomedical materials.

[169]  A. Gaharwar,et al.  Advanced Bioinks for 3D Printing: A Materials Science Perspective , 2016, Annals of Biomedical Engineering.

[170]  M. Barbeck,et al.  Bioprinting of tissue engineering scaffolds , 2018, Journal of tissue engineering.

[171]  Xuejia Ding,et al.  Polycaprolactone/polysaccharide functional composites for low-temperature fused deposition modelling , 2020, Bioactive materials.

[172]  Udayabhanu M. Jammalamadaka,et al.  Novel Biomaterials Used in Medical 3D Printing Techniques , 2018, Journal of functional biomaterials.

[173]  G. Klein,et al.  3D printing and neurosurgery--ready for prime time? , 2013, World neurosurgery.

[174]  G. Pei,et al.  3D-bioprinted functional and biomimetic hydrogel scaffolds incorporated with nanosilicates to promote bone healing in rat calvarial defect model. , 2020, Materials science & engineering. C, Materials for biological applications.

[175]  V. Thakur,et al.  Recent progress in cellulose nanocrystals: sources and production. , 2017, Nanoscale.

[176]  V. Brunella,et al.  Synthesis of poly(N‐isopropylacrylamide) by distillation precipitation polymerization and quantitative grafting on mesoporous silica , 2016 .

[177]  Abuliti Abudula,et al.  Nanocellulose: Extraction and application , 2018 .

[178]  Xiongbiao Chen,et al.  Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters , 2019 .

[179]  Young-Jin Kim,et al.  3D Bioprinting Technologies for Tissue Engineering Applications. , 2018, Advances in experimental medicine and biology.

[180]  Anthony Atala,et al.  3D bioprinting of tissues and organs , 2014, Nature Biotechnology.

[181]  H. Santos,et al.  Acetylated Nanocellulose for Single-Component Bioinks and Cell Proliferation on 3D-Printed Scaffolds , 2019, Biomacromolecules.

[182]  Zhao Li,et al.  Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior , 2018, Scientific Reports.

[183]  Malcolm Xing,et al.  3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances , 2018, Bioactive materials.

[184]  Chuanbo Guo,et al.  Impacts of hatchery-reared mandarin fish Siniperca chuatsi stocking on wild fish community and water quality in a shallow Yangtze lake , 2018, Scientific Reports.

[185]  E. Ioannou,et al.  Ulvan, a bioactive marine sulphated polysaccharide as a key constituent of hybrid biomaterials: A review. , 2019, Carbohydrate polymers.

[186]  L. Bertassoni,et al.  A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry , 2018, Biofabrication.

[187]  A. Boccaccini,et al.  Cell-laden alginate dialdehyde–gelatin hydrogels formed in 3D printed sacrificial gel , 2020, Journal of Materials Science: Materials in Medicine.

[188]  Bikramjit Basu,et al.  Microstructure and compression properties of 3D powder printed Ti-6Al-4V scaffolds with designed porosity: Experimental and computational analysis. , 2017, Materials science & engineering. C, Materials for biological applications.

[189]  S. Reis,et al.  Marine Polysaccharides in Pharmaceutical Applications: Fucoidan and Chitosan as Key Players in the Drug Delivery Match Field , 2019, Marine drugs.

[190]  Woong Sun,et al.  Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors , 2011, Proceedings of the National Academy of Sciences.

[191]  Mehmet Remzi Dokmeci,et al.  Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks , 2019, Micromachines.

[192]  Ali Khademhosseini,et al.  Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs , 2019, Materials today. Bio.

[193]  Haishun Du,et al.  Cellulose nanocrystals and cellulose nanofibrils based hydrogels for biomedical applications. , 2019, Carbohydrate polymers.

[194]  R. Jayakumar,et al.  Carrageenan based hydrogels for drug delivery, tissue engineering and wound healing. , 2018, Carbohydrate polymers.

[195]  V. Pillay,et al.  Poly(ethylene glycol) enclatherated pectin-mucin submicron matrices for intravaginal anti-HIV-1 drug delivery. , 2016, International journal of pharmaceutics.

[196]  Johnson H. Y. Chung,et al.  Synthesis and 3D Printing of Conducting Alginate–Polypyrrole Ionomers , 2020, Gels.

[197]  M. Ramalingam,et al.  Designing vascular supportive albumen-rich composite bioink for organ 3D printing. , 2020, Journal of the mechanical behavior of biomedical materials.

[198]  P. R. van Weeren,et al.  Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs. , 2013, Macromolecular bioscience.

[199]  V. Raghuwanshi,et al.  Engineering nanocellulose hydrogels for biomedical applications. , 2019, Advances in colloid and interface science.

[200]  P. Gatenholm,et al.  3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications. , 2015, Biomacromolecules.

[201]  S. Van Vlierberghe,et al.  Extrusion-based 3D printing of photo-crosslinkable gelatin and κ-carrageenan hydrogel blends for adipose tissue regeneration. , 2019, International journal of biological macromolecules.

[202]  Ali Khademhosseini,et al.  Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments , 2018, Biofabrication.

[203]  B. Kloareg,et al.  Degradation of λ-carrageenan by Pseudoalteromonas carrageenovora λ-carrageenase: a new family of glycoside hydrolases unrelated to κ- and ι-carrageenases , 2007 .

[204]  Ya-Qi Xue,et al.  Enhancement of mechanical strength of TCP-alginate based bioprinted constructs. , 2019, Journal of the mechanical behavior of biomedical materials.

[205]  Sangwon Chung,et al.  Hierarchical starch‐based fibrous scaffold for bone tissue engineering applications , 2009, Journal of tissue engineering and regenerative medicine.

[206]  A. Khademhosseini,et al.  Interpenetrating network gelatin methacryloyl (GelMA) and pectin-g-PCL hydrogels with tunable properties for tissue engineering. , 2018, Biomaterials science.

[207]  Miguel Alaminos,et al.  Generation of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applications , 2017, Biomedical materials.

[208]  Udayabhanu M. Jammalamadaka,et al.  Recent Advances in Biomaterials for 3D Printing and Tissue Engineering , 2018, Journal of functional biomaterials.

[209]  S. Ray,et al.  A 3D printed chitosan-pectin hydrogel wound dressing for lidocaine hydrochloride delivery. , 2019, Materials science & engineering. C, Materials for biological applications.

[210]  Scott A. Wilson,et al.  Shear-Thinning and Thermo-Reversible Nanoengineered Inks for 3D Bioprinting. , 2017, ACS applied materials & interfaces.

[211]  Rui Luís Reis,et al.  Seaweed polysaccharide-based hydrogels used for the regeneration of articular cartilage , 2015, Critical reviews in biotechnology.

[212]  LetourneurDidier,et al.  Fucoidan promotes early step of cardiac differentiation from human embryonic stem cells and long-term maintenance of beating areas. , 2014 .

[213]  Jae Young Lee,et al.  Cell-laden 3D bioprinting hydrogel matrix depending on different compositions for soft tissue engineering: Characterization and evaluation. , 2017, Materials science & engineering. C, Materials for biological applications.

[214]  D. Hutmacher,et al.  Scaffold development using 3D printing with a starch-based polymer , 2002 .

[215]  P. Gatenholm,et al.  Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo , 2018, Plastic and reconstructive surgery. Global open.

[216]  T. Maver,et al.  Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis , 2020, Nanomaterials.