A microfluidic-photonic-integrated device with enhanced excitation power density

The power density of optical excitation in microfluidic-photonic-integrated flow cytometers is typically provided from an integrated waveguide and the beam is therefore divergent within the microchannel due to the NA of the waveguide; a detrimental effect on detection capabilities as excitation is not uniform throughout the channel and will generate a long pulse for excitation. Through integration of a lens system specially designed and simulated to collect and reshape 100% of input power, the excitation power within the microchannel has been controlled to form an optimal spot size within the microchannel. The device was formed via a one-shot processing method where designs are patterned into a SU-8 layer on a Pyrex substrate. A poly(dimethylsiloxane) (PDMS) layer was used to seal the device and serve as an upper cladding for integrated waveguides. Spot sizes were improved from an unfocused width of 86um to less than 40um. Power densities were controlled throughout the width of the channel - an improvement for flow cytometry applications.

[1]  Luke P. Lee,et al.  Disposable integrated microfluidics with self-aligned planar microlenses , 2004 .

[2]  H Fujita,et al.  PDMS 2D optical lens integrated with microfluidic channels: principle and characterization. , 2003, Lab on a chip.

[3]  Howard M. Shapiro,et al.  Practical Flow Cytometry , 1985 .

[4]  C. Bliss,et al.  Integrated wavelength-selective optical waveguides for microfluidic-based laser-induced fluorescence detection. , 2008, Lab on a chip.

[5]  D. Bradley,et al.  Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection. , 2006, Lab on a chip.

[6]  G. Whitesides,et al.  Diagnostics for the developing world: microfluidic paper-based analytical devices. , 2010, Analytical chemistry.

[7]  B. Robertson,et al.  New microbiology tools for public health and their implications. , 2005, Annual review of public health.

[8]  Hywel Morgan,et al.  High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection. , 2006, Biosensors & bioelectronics.

[9]  A. M. Jorgensen,et al.  Lab-on-a-chip with integrated optical transducers. , 2006, Lab on a chip.

[10]  Ryuji Koyama,et al.  Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay. , 2003, Lab on a chip.

[11]  Alex Groisman,et al.  High-throughput and high-resolution flow cytometry in molded microfluidic devices. , 2006, Analytical chemistry.

[12]  K. Mogensen,et al.  Integration of polymer waveguides for optical detection in microfabricated chemical analysis systems. , 2003, Applied optics.

[13]  S. Quake,et al.  An Integrated Microfabricated Cell Sorter , 2022 .