Molecular size of the canine and human brain D2 dopamine receptor as determined by radiation inactivation.

Target-size analysis (radiation inactivation) has been utilized for determination of the molecular size of the striatal D2 dopamine receptor of both canine and human membranes. The dog and human receptors were found to have a molecular size of 123,000 daltons. The identity of molecular size values is consistent with available pharmacological and biochemical evidence supporting D2 dopamine receptor identity in canine and human tissues. These data suggest that the canine receptor may be a valid model for molecular and structural investigation of the human D2 dopamine receptor.

[1]  P. Seeman,et al.  Dopamine receptor sites and states in human brain. , 1983, Journal of neural transmission. Supplementum.

[2]  J. Venter,et al.  Autoantibodies and monoclonal antibodies in the purification and molecular characterization of neurotransmitter receptors , 1983, Journal of cellular biochemistry.

[3]  J. Venter,et al.  The size of the mammalian lung beta 2-adrenergic receptor as determined by target size analysis and immunoaffinity chromatography. , 1982, Biochemical and biophysical research communications.

[4]  L. Iversen,et al.  Molecular size of benzodiazepine receptor in rat brain in situ: evidence for a functional dimer? , 1982, Nature.

[5]  S. Paul,et al.  In situ molecular weight determination of brain and peripheral benzodiazepine binding sites. , 1981, European journal of pharmacology.

[6]  P. Seeman,et al.  Resolution of dopamine and serotonin receptor components of [3H]spiperone binding to rat brain regions. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[7]  P. Seeman,et al.  Solubilization of neuroleptic/dopamine receptors of human brain striatum. , 1981, European journal of pharmacology.

[8]  W. Schlegel,et al.  Structure of the turkey erythrocyte adenylate cyclase system. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[9]  P. Seeman.  Brain dopamine receptors. , 1980, Pharmacological reviews.

[10]  C. Kahn,et al.  Characterization of the insulin receptor in its membrane environment by radiation inactivation. , 1980, The Journal of biological chemistry.

[11]  E S Kempner,et al.  Size determination of enzymes by radiation inactivation. , 1979, Analytical biochemistry.

[12]  L. Iversen,et al.  Use of ADTN to define specific 3H-spiperone binding to receptors in brain , 1978, Nature.

[13]  J. Leysen,et al.  A serotonergic component of neuroleptic receptors. , 1977, Archives internationales de pharmacodynamie et de therapie.

[14]  B. Vallee,et al.  ZINC, A COMPONENT OF YEAST ALCOHOL DEHYDROGENASE. , 1955, Proceedings of the National Academy of Sciences of the United States of America.

[15]  E. Pollard.  Primary ionization as a test of molecular organization. , 1953, Advances in biological and medical physics.

[16]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.