Investigations into the role of bacteria/dinoflagellate interactions in Paralytic Shellfish Poisoning

Fluorescently-labelled 16S and 23S rRNA oligodeoxynucleotide probes, group or clade specific, respectively, were used to identify bacteria associated with Alexandrium spp. by confocal laser scanning microscopy (CLSM). Bacteria were found to be associated with Alexandrium tamarense, A. lusitanicum and A. andersonii, intraand/ or extracellulary, respectively. The presence of metabolically active intracellular bacteria in dinoflagellates of the genus Alexandrium was documented with the use of 5-cyano-2,3-ditolyl tetrazolium chloride (CTC). This compound is reduced by living, respiring bacteria into a water-insoluble fluorescent formazan (CTF), which fluorescences bright red. Actively respiring bacteria were detected intracellularly in log and stationary phase-fixed A. tamarense and A. lusitanicum by epifluorescence microscopy (FM) and CLSM. These results support the given theory that specific bacteria are an integral part of the physical environment of Alexandrium spp.

[1]  Stuart Carroll,et al.  The production of tetrodotoxin-like substances by nemertean worms in conjunction with bacteria , 2003 .

[2]  Elizabeth A. Smith,et al.  Kinetics of attachment of potentially toxic bacteria to Alexandrium tamarense , 2002 .

[3]  D. Vaulot,et al.  IDENTIFICATION OF BACTERIA ASSOCIATED WITH DINOFLAGELLATES (DINOPHYCEAE) ALEXANDRIUM SPP. USING TYRAMIDE SIGNAL AMPLIFICATION–FLUORESCENT IN SITU HYBRIDIZATION AND CONFOCAL MICROSCOPY1 , 2002 .

[4]  L. Cárdenas,et al.  Multiple bacterial infection ofAlexandrium catenella (Dinophyceae) , 2002 .

[5]  Y. Sako,et al.  PURIFICATION AND CHARACTERIZATION OF A SULFOTRANSFERASE SPECIFIC TO N‐21 OF SAXITOXIN AND GONYAUTOXIN 2+3 FROM THE TOXIC DINOFLAGELLATE GYMNODINIUM CATENATUM (DINOPHYCEAE) , 2001 .

[6]  Elizabeth A. Smith,et al.  Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates: Alexa , 2001 .

[7]  A. Wichels,et al.  Diversity of endocytic bacteria in the dinoflagellate Noctiluca scintillans , 2001 .

[8]  Todd R. Miller,et al.  Bacterial community associated with Pfiesteria-like dinoflagellate cultures. , 2001, Environmental microbiology.

[9]  S. França,et al.  Bacterium-dinoflagellate interactions: investigative microscopy of Alexandrium spp. (Gonyaulacales, Dinophyceae) , 2001 .

[10]  Elizabeth A. Smith,et al.  Biotransformations of Paralytic Shellfish Toxins by Bacteria Isolated from Bivalve Molluscs , 2001, Applied and Environmental Microbiology.

[11]  P. Vale,et al.  Determination of paralytic shellfish toxins in Portuguese shellfish by automated pre-column oxidation. , 2001, Toxicon : official journal of the International Society on Toxinology.

[12]  T. Narahashi.  PHARMACOLOGY OF TETRODOTOXIN , 2001 .

[13]  D. Hwang,et al.  Isolation of bacteria from toxic dinoflagellate Alexandrium minutum and their effects on algae toxicity. , 2000, Journal of natural toxins.

[14]  B. Luckas.  Chemical Analysis of PSP Toxins , 2000 .

[15]  R. Amann,et al.  16S rRNA Targeted Probes for the Identification of Bacterial Strains Isolated from Cultures of the Toxic Dinoflagellate Alexandrium tamarense , 2000, Microbial Ecology.

[16]  P. Truquet,et al.  Comparative efficiencies of different non-toxic microalgal diets in detoxification of PSP-contaminated oysters (Crassostrea gigas Thunberg). , 2000, Journal of natural toxins.

[17]  S. Gallacher,et al.  Marine toxins. , 2000, British medical bulletin.

[18]  H. Grossart.  Interactions between marine bacteria and axenic diatoms (Cylindrotheca fusiformis, Nitzschia laevis, and Thalassiosira weissflogii) incubated under various conditions in the lab , 1999 .

[19]  U. Szewzyk,et al.  Phylogenetic Composition, Spatial Structure, and Dynamics of Lotic Bacterial Biofilms Investigated by Fluorescent in Situ Hybridization and Confocal Laser Scanning Microscopy , 1999, Microbial Ecology.

[20]  R. Amann,et al.  In Situ Identification of Cyanobacteria with Horseradish Peroxidase-Labeled, rRNA-Targeted Oligonucleotide Probes , 1999, Applied and Environmental Microbiology.

[21]  G. Hold.  The role of bacteria in paralytic shellfish poisoning , 1999 .

[22]  C. Schütt,et al.  Intracellular bacteria in the red tide-forming heterotrophic dinoflagellate Noctiluca scintillans , 1999 .

[23]  Mitchell,et al.  Microscale nutrient patches in planktonic habitats shown by chemotactic bacteria , 1998, Science.

[24]  G. Gerdts,et al.  Bacteria of the Genus Roseobacter Associated with the Toxic Dinoflagellate Prorocentrum lima. , 1998, Protist.

[25]  G. Hallegraeff,et al.  Algicidal Effects of a Novel MarinePseudoalteromonas Isolate (Class Proteobacteria, Gamma Subdivision) on Harmful Algal Bloom Species of the GeneraChattonella, Gymnodinium, andHeterosigma , 1998, Applied and Environmental Microbiology.

[26]  Farooq Azam,et al.  Microbial Control of Oceanic Carbon Flux: The Plot Thickens , 1998, Science.

[27]  Y. Ishida,et al.  Detection and enumeration of microorganisms that are lethal to harmful phytoplankton in coastal waters , 1998 .

[28]  W. Carmichael,et al.  New saxitoxin analogues from the freshwater filamentous cyanobacterium Lyngbya wollei. , 1998, Natural toxins.

[29]  G. Gerdts,et al.  Phylogenetic analysis of selected toxic and non-toxic bacterial strains isolated from the toxic dinoflagellate Alexandrium tamarense , 1997 .

[30]  W. Carmichael,et al.  Evidence for paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei (Farlow ex Gomont) comb. nov , 1997, Applied and environmental microbiology.

[31]  D. Anderson,et al.  Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense , 1997 .

[32]  H. Hines,et al.  Evidence for production of paralytic shellfish toxins by bacteria associated with Alexandrium spp. (Dinophyta) in culture , 1997, Applied and environmental microbiology.

[33]  C. Ménard,et al.  Determination of decarbamoyl saxitoxin and its analogues in shellfish by prechromatographic oxidation and liquid chromatography with fluorescence detection. , 1996, Journal of AOAC International.

[34]  D. Anderson,et al.  WIDESPREAD PHAGOCYTOSIS OF CILIATES AND OTHER PROTISTS BY MARINE MIXOTROPHIC AND HETEROTROPHIC THECATE DINOFLAGELLATES 1 , 1996 .

[35]  T. Noguchi,et al.  Microflora and tetrodotoxin-producing bacteria in a gastropod, Niotha clathrata. , 1995, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[36]  M. Soyer-Gobillard,et al.  The toxic dinoflagellate Prorocentrum lima and its associated bacteria: I. An ultrastructural study , 1995 .

[37]  G. Doucette,et al.  Interactions between bacteria and harmful algae: a review. , 1995, Natural toxins.

[38]  G. Doucette,et al.  Characterization of bacteria associated with different isolates of Alexandrium tamarense , 1995 .

[39]  Gustaaf M. Hallegraeff,et al.  Harmful algal blooms: a global overview , 1995 .

[40]  D. Anderson,et al.  IDENTIFICATION OF GROUP‐ AND STRAIN‐SPECIFIC GENETIC MARKERS FOR GLOBALLY DISTRIBUTED ALEXANDRIUM (DINOPHYCEAE). I. RFLP ANALYSIS OF SSU rRNA GENES 1 , 1994 .

[41]  V. V. Sastry,et al.  Antibacterial Substances from Marine Algae: Successive Extraction Using Benzene, Chloroform and Methanol , 1994 .

[42]  Egbert J. de Vries,et al.  Isolation of Typical Marine Bacteria by Dilution Culture: Growth, Maintenance, and Characteristics of Isolates under Laboratory Conditions , 1993, Applied and environmental microbiology.

[43]  Y. Sako,et al.  Variation of Toxin Production and Composition in Axenic Cultures of Alexandrium catenella and A.tamarense. , 1993 .

[44]  Wayne W. Carmichael,et al.  Diseases related to freshwater blue-green algal toxins, and control measures , 1993 .

[45]  A. Cembella,et al.  Anatomical distribution and spatio-temporal variation in paralytic shellfish toxin composition in two bivalve species from the Gulf of Maine , 1993 .

[46]  G. Hallegraeff,et al.  Toxin composition of resting cysts of Alexandrium tamarense (Dinophyceae). , 1992, Toxicon : official journal of the International Society on Toxinology.

[47]  J. Stewart,et al.  Paralytic shellfish poison (saxitoxin family) bioassays: automated endpoint determination and standardization of the in vitro tissue culture bioassay, and comparison with the standard mouse bioassay. , 1992, Toxicon : official journal of the International Society on Toxinology.

[48]  K. Rothhaupt,et al.  The influence of spatial and temporal concentration gradients on phosphate partitioning between different size fractions of plankton: Further evidence and possible causes , 1992 .

[49]  Y. Ishida,et al.  Isolation of a marine gliding bacterium that kills Chattonella antiqua (Raphidophyceae) , 1991 .

[50]  A. Sournia,et al.  Marine phytoplankton: how many species in the world ocean? , 1991 .

[51]  C. Duarte,et al.  Influence of algal population dynamics on phytoplankton colonization by bacteria: evidence from two diatom species , 1990 .

[52]  M. Soyer-Gobillard,et al.  Bacteria Associated with a Photosynthetic Dinoflagellate in Culture , 1990 .

[53]  T. Sakata.  Occurrence of Marine Saprospira sp. Possessing Algicidal Activity for Diatoms , 1990 .

[54]  D. Anderson,et al.  Uptake of Alexandrium fundyense by Mytilus edulis and Mercenaria mercenaria under controlled conditions , 1990 .

[55]  D. Kulis,et al.  Toxin composition variations in one isolate of the dinoflagellate Alexandrium fundyense. , 1990, Toxicon : official journal of the International Society on Toxinology.

[56]  S. Sato,et al.  Production of paralytic shellfish toxins by a bacterium Moraxella sp. isolated from Protogonyaulax tamarensis. , 1990, Toxicon : official journal of the International Society on Toxinology.

[57]  D. Anderson,et al.  Toxic dinoflagellates and marine mammal mortalities : proceedings of an expert consultation held at the Woods Hole Oceanographic Institution , 1989 .

[58]  D. Anderson,et al.  Humpback Whales (Megaptera novaeangliae) Fatally Poisoned by Dinoflagellate Toxin , 1989 .

[59]  R. Pierce,et al.  Bacteriological aspects of Florida red tides: A revisit and newer obervations , 1989 .

[60]  D. Ballantine,et al.  Associated bacterial flora, growth, and toxicity of cultured benthic dinoflagellates Ostreopsis lenticularis and Gambierdiscus toxicus , 1989, Applied and environmental microbiology.

[61]  Y. Kotaki.  Screening of Bacteria which Convert Gonyautoxin 2, 3 to Saxitoxin , 1989 .

[62]  T. Barbeyron,et al.  Commensual bacteria living two multicellular marine algae Antithamnion plumula (Ellis) Thuret and Cladophora rupestris (L.) Kützing (Linné), Kützing. Phenotypic characterization , 1989 .

[63]  Shigeru Sato,et al.  Bacterial Production of Saxitoxin , 1988 .

[64]  S. França,et al.  The association dinoflagellate-bacteria: their ultrastructural relationship in two species of dinoflagellates , 1985 .

[65]  S. Hall.  Toxins and toxicity of Protogonyaulax from the Northeast Pacific , 1982 .

[66]  Jonathan J. Cole,et al.  INTERACTIONS BETWEEN BACTERIA AND ALGAE IN AQUATIC ECOSYSTEMS , 1982 .

[67]  H. Iwasaki.  12 – Physiological Ecology of Red Tide Flagellates , 1979 .

[68]  H. Hoppe.  Relations between active bacteria and heterotrophic potential in the sea , 1978 .

[69]  B. Dale,et al.  Red Tide and Paralytic Shellfish Poisoning. , 1978 .

[70]  A. H. Meinke,et al.  Paralytic shellfish poisoning. , 1973, Michigan medicine.

[71]  Ralph Mitchell,et al.  Studies in Bacterial Chemoreception. I. The Effect of Biogenic Amines and Cannabinoids on Bacterial Chemoreception. II. Chemotactic and Growth Responses of Marine Bacteria to Algal Extracellular Products. , 1972 .