THE PRODUCTION OF TRIPLOID BROWN TROUT ( SALMO TRUTTA ) USING CAFFEINE TREATMENT

In the present paper suitable conditions were determined for caffeine treatment that is safe and inexpensive for the induction of triploid brown trout. To suppress the second meiotic division, fertilized eggs were exposed to three different concentrations (510 and 15-mM) caffeine solution for 10 min beginning at 15 min after fertilization. After that, the eggs were incubated at ambient temperature until hatching. Incidence of triploid fry was determined from flow-cytometric analysis. There was no significant difference in the mean triploid rates all caffeine treatments and not observed successful results in these groups.