Development of a Surface-Functionalized Titanium Implant for Promoting Osseointegration: Surface Characteristics, Hemocompatibility, and In Vivo Evaluation

This study aimed to evaluate the impact of surface-modified biomedical titanium (Ti) dental implant on osseointegration. The surfaces were modified using an innovative dip-coating technique (IDCT; sandblasted, large-grit, and acid-etched, then followed by coating with the modified pluronic F127 biodegradable polymer). The surface morphology and hemocompatibility evaluations were investigated by field-emission scanning electron microscopy, while the contact analysis was observed by goniometer. The IDCT-modified Ti implant was also implanted in patients with missing teeth by single-stage surgical procedure then observed immediately and again four months after placement by cone-beam computerized tomography (CBCT) imaging. It was found that the IDCT-modified Ti implant was rougher than the dental implant without surface modification. Contact angle analysis showed the IDCT-modified Ti implant was lower than the dental implant without surface modification. The hemocompatibility evaluations showed greater red blood cell aggregation and fibrin filament formation on the IDCT-modified Ti implant. The radiographic and CBCT image displayed new bone formation at four months after the IDCT-modified Ti implant placement. Therefore, this study suggests that the IDCT-modified Ti dental implant has great potential to accelerate osseointegration.

[1]  M. Alam,et al.  Bacterial Colonization and Dental Implants: A Microbiological Study , 2020 .

[2]  Shi-feng Liu,et al.  Multi-Scale Surface Treatments of Titanium Implants for Rapid Osseointegration: A Review , 2020, Nanomaterials.

[3]  Heng-Li Huang,et al.  Risk Factors related to Late Failure of Dental Implant—A Systematic Review of Recent Studies , 2020, International journal of environmental research and public health.

[4]  M. Çelik,et al.  Wetting properties of blood lipid fractions on different titanium surfaces , 2020, International journal of implant dentistry.

[5]  C. Maiorana,et al.  Multidisciplinary Oral Rehabilitation of a Severely Compromised Dentition , 2020, Case reports in dentistry.

[6]  P. Gentile,et al.  Surface Characterization of Electro-Assisted Titanium Implants: A Multi-Technique Approach , 2020, Materials.

[7]  Pierre P. D. Kondiah,et al.  A 3D Bioprinted Pseudo-Bone Drug Delivery Scaffold for Bone Tissue Engineering , 2020, Pharmaceutics.

[8]  M. Bianco,et al.  Do Dietary Supplements and Nutraceuticals Have Effects on Dental Implant Osseointegration? A Scoping Review , 2020, Nutrients.

[9]  I. Yeo.  Modifications of Dental Implant Surfaces at the Micro- and Nano-Level for Enhanced Osseointegration , 2019, Materials.

[10]  C. Villa,et al.  Poloxamer Hydrogels for Biomedical Applications , 2019, Pharmaceutics.

[11]  Hom-lay Wang,et al.  A Systematic Review of Survival Rates of Osseointegrated Implants in Fully and Partially Edentulous Patients Following Immediate Loading , 2019, Journal of clinical medicine.

[12]  Danyal A. Siddiqui,et al.  Biological characterization of surface-treated dental implant materials in contact with mammalian host and bacterial cells: titanium versus zirconia , 2019, RSC advances.

[13]  G. Macaluso,et al.  Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization , 2019, Nanomaterials.

[14]  G. Risitano,et al.  Sandblasted and Acid Etched Titanium Dental Implant Surfaces Systematic Review and Confocal Microscopy Evaluation , 2019, Materials.

[15]  M. T. Cidade,et al.  Injectable Hydrogels Based on Pluronic/Water Systems Filled with Alginate Microparticles for Biomedical Applications , 2019, Materials.

[16]  Alberto Bianchi,et al.  Bioactive Titanium Surfaces: Interactions of Eukaryotic and Prokaryotic Cells of Nano Devices Applied to Dental Practice , 2019, Biomedicines.

[17]  G. Yadegarfar,et al.  Dental Implant Rehabilitation in Patients Suffering from Mucocutaneous Diseases: A Systematic Review and Meta-Analysis , 2018, The Open Dentistry Journal.

[18]  K. Ou,et al.  Evaluation of Surface Characteristics and Hemocompatibility on the Oxygen Plasma-Modified Biomedical Titanium , 2018, Metals.

[19]  Shuai Jiang,et al.  Accelerated and enhanced osteointegration of MAO-treated implants: histological and histomorphometric evaluation in a rabbit model , 2018, International Journal of Oral Science.

[20]  K. Ou,et al.  Hybrid micro/nanostructural surface offering improved stress distribution and enhanced osseointegration properties of the biomedical titanium implant. , 2018, Journal of the mechanical behavior of biomedical materials.

[21]  J. Malmström,et al.  Simple Coatings to Render Polystyrene Protein Resistant , 2018 .

[22]  H. Engqvist,et al.  Classification and Effects of Implant Surface Modification on the Bone: Human Cell‐Based In Vitro Studies , 2017, The Journal of oral implantology.

[23]  Wei Li,et al.  Incorporating simvastatin/poloxamer 407 hydrogel into 3D-printed porous Ti6Al4V scaffolds for the promotion of angiogenesis, osseointegration and bone ingrowth , 2016, Biofabrication.

[24]  F. Kloss,et al.  Impact of Dental Implant Surface Modifications on Osseointegration , 2016, BioMed research international.

[25]  F. Mussano,et al.  Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology , 2016 .

[26]  Y. Otsuka,et al.  Surface Modifications and Their Effects on Titanium Dental Implants , 2015, BioMed research international.

[27]  Kanwal Rehman,et al.  Recent progress in biomedical applications of Pluronic (PF127): Pharmaceutical perspectives. , 2015, Journal of controlled release : official journal of the Controlled Release Society.

[28]  J. Granjeiro,et al.  Early osseointegration driven by the surface chemistry and wettability of dental implants , 2015, Journal of applied oral science : revista FOB.

[29]  A. Thor,et al.  A hydrophilic dental implant surface exhibits thrombogenic properties in vitro. , 2013, Clinical implant dentistry and related research.

[30]  A. Palotás,et al.  Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers , 2012, International journal of nanomedicine.

[31]  Hee-Jin Kim,et al.  Surface characteristics of a novel hydroxyapatite-coated dental implant , 2012, Journal of periodontal & implant science.

[32]  P. Vallittu,et al.  The Effect of Exposed Glass Fibers and Particles of Bioactive Glass on the Surface Wettability of Composite Implants , 2011, International journal of biomaterials.

[33]  K. Gulati,et al.  Controlling Drug Release from Titania Nanotube Arrays Using Polymer Nanocarriers and Biopolymer Coating , 2011 .

[34]  L. Scheideler,et al.  Wetting behavior of dental implants. , 2011, The International journal of oral & maxillofacial implants.

[35]  Waham Ashaier Laftah,et al.  Polymer Hydrogels: A Review , 2011 .

[36]  M. Çulha,et al.  Pluronic block copolymer-mediated interactions of organic compounds with noble metal nanoparticles for SERS analysis. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[37]  O. Marti,et al.  Usage of polymer brushes as substrates of bone cells , 2009 .

[38]  L. Levin.  DEALING WITH DENTAL IMPLANT FAILURES , 2008, Journal of applied oral science : revista FOB.

[39]  P. Layrolle,et al.  Surface treatments of titanium dental implants for rapid osseointegration. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[40]  J. Davies,et al.  Red blood cell and platelet interactions with titanium implant surfaces. , 2000, Clinical oral implants research.

[41]  M. Uthappa,et al.  Surface modifications of titanium implants – The new, the old, and the never heard of options , 2016 .

[42]  G. Strnad,et al.  Contact Angle Measurement on Medical Implant Titanium Based Biomaterials , 2016 .

[43]  V. Chiono,et al.  Pluronic F127 Hydrogel Characterization and Biofabrication in Cellularized Constructs for Tissue Engineering Applications , 2015 .

[44]  E. A. Ahmed,et al.  The Survival Rate of RBM Surface versus SLA Surface in Geometrically Identical Implant Design , 2015 .

[45]  H. Hosseinkhani,et al.  Comparison of Cell Response and Surface Characteristics on Titanium Implant with SLA and SLAffinity Functionalization , 2014 .

[46]  P. Shukla,et al.  Surface topography of dental implants: A review , 2014 .

[47]  M. Lourenço,et al.  Synthesis and antimicrobial activity of amphiphilic carbohydrate derivatives , 2008 .

[48]  A. Mata,et al.  Hybrid bone implants: self-assembly of peptide amphiphile nanofibers within porous titanium. , 2008, Biomaterials.