Immunological cross reactions betweenAeromonas salmonicidaandVibrio salmonicidain Atlantic salmon (Salmo salarL.) and rabbit

Abstract Immunological cross reactions between Aeromonas salmonicida and three species of Vibrio were evaluated. Each of five groups of Atlantic salmon were injected with one of the following oil-adjuvanted bacterins: Aeromonas salmonicida ss. salmonicida , Vibrio salmonicida , Vibrio anguillarum serovar O1 and Vibrio anguillarum serovar O2 or with placebo. In addition, rabbits were immunised with the same oil-adjuvanted bacterins of A. salmonicida or V. salmonicida . The levels of specific antibodies against A. salmonicida antigens were measured in an ELISA, and the responses were characterised in Western blotting and crossed immunoelectrophoresis (CIE). Protective immunity against furunculosis in the various groups of Atlantic salmon was measured in a virulent cohabitant challenge. Atlantic salmon and rabbit vaccinated against V. salmonicida produced antibodies that cross-reacted with antigen preparations of A. salmonicida in ELISA, Western blotting and CIE (rabbit only). CIE and Western blotting showed that rabbit anti- V. salmonicida reacted with antigens on A. salmonicida other than the homologous antisera. Fish immunised against V. salmonicida were significantly protected in a cohabitant A. salmonicida challenge. These cross reactions may explain the superior protection against furunculosis achieved with polyvalent vaccines against furunculosis, vibriosis and cold water vibriosis compared to a monovalent A. salmonicida vaccine.

[1]  R. Nordmo,et al.  Comparison of different challenge methods to evaluate the efficacy of furunculosis vaccines in Atlantic salmon, Salmo salar L. , 1997 .

[2]  A. Lillehaug,et al.  Vibrioantigens of polyvalent vaccines enhance the humoral immune response toAeromonas salmonicidaantigens in Atlantic salmon (Salmo salarL.) , 1997 .

[3]  A. Ellis,et al.  Antibody response and protection of Atlantic salmon (Salmo salar) immunised with an extracellular polysaccharide ofAeromonas salmonicida , 1997 .

[4]  P. Midtlyng,et al.  Experimental studies on the efficacy and side-effects of intraperitoneal vaccination of Atlantic salmon (Salmo salarL.) against furunculosis , 1996 .

[5]  C. Locht,et al.  European Commission COST/STD Initiative. Report of the expert panel VIII. New vaccines, especially new combined vaccines. , 1996, Vaccine.

[6]  C. Press,et al.  Vaccination in European salmonid aquaculture: a review of practices and prospects. , 1995, The British veterinary journal.

[7]  B. Robertsen,et al.  Adjuvant effect of a yeast glucan in vaccines against furunculosis in Atlantic salmon (Salmo salar L.) , 1993 .

[8]  L. Reitan,et al.  Immune response and protective immunity after vaccination of Atlantic salmon (Salmo salar L.) against furunculosis. , 1992 .

[9]  Douglas P. Anderson.  Immunostimulants, adjuvants, and vaccine carriers in fish: applications to aquaculture , 1992 .

[10]  T. Jørgensen,et al.  Humoral immune response in Atlantic salmon, Salmo salar L., to cellular and extracellular antigens of Aeromonas salmonicida , 1991 .

[11]  J. Bøgwald,et al.  Antibody specificities in Atlantic salmon, Salmo salar L., against the fish pathogens Vibrio salmonicida and Vibrio anguillarum , 1991 .

[12]  C. Fossum,et al.  Monoclonal antibodies to salmonid immunoglobulin: characterization and applicability in immunoassays. , 1990, Developmental and comparative immunology.

[13]  A. Ellis,et al.  Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccine preparations. , 1990 .

[14]  C. Endresen,et al.  Specific immunoglobulins in serum from Atlantic salmon, Salmo salar L., immunized with Vibrio salmonicida and infectious pancreatic necrosis virus , 1990 .

[15]  A. Lillehaug.  A field trial of vaccination against cold-water vibriosis in Atlantic salmon (Salmo salar L.). , 1990 .

[16]  A. Ellis,et al.  The humoral immune response of rainbow trout (Salmo gairdneri) immunised by various regimes and preparations of Aeromonas salmonicida antigens. , 1988, Veterinary immunology and immunopathology.

[17]  B. M. Phipps,et al.  Immunoglobulin binding by the regular surface array of Aeromonas salmonicida. , 1988, The Journal of biological chemistry.

[18]  M. T. Horne,et al.  The potency of adjuvanted injected vaccines in rainbow trout (Salmo gairdneri Richardson) and bath vaccines in Atlantic salmon (Salmo salar L.) against furunculosis , 1988 .

[19]  A. Ellis,et al.  The humoral immune response of rainbow trout, Salmo gairdneri Richardson, and rabbits to Aeromonas salmonicida extracellular products , 1988 .

[20]  D. Amend,et al.  Evidence for lack of antigenic competition among various combinations of Vibrio anguillarum, Yersinia ruckeri, Aeromonas salmonicida and Renibacterium salmoninarum bacterins when administered to salmonid fishes , 1984 .

[21]  S. Pocock,et al.  Clinical Trials: A Practical Approach , 1984 .

[22]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[23]  Paul J. B. Hart,et al.  Field Trials of Fish Marking using a Jet Inoculator , 1969 .

[24]  J. Fryer,et al.  ACTIVE AND PASSIVE IMMUNIZATION OF CERTAIN SALMONID FISHES AGAINST AEROMONAS SALMONICIDA. , 1965, Canadian journal of microbiology.