A soluble extract from human spermatozoa activates ascidian oocytes

A soluble extract from human spermatozoa induced calcium oscillations and extrusion of the first polar body when injected into oocytes of the ascidian Ciona intestinalis. The properties of calcium oscillations and time of polar body extrusion precisely mimic oocyte activation induced by C. intestinalis sperm or sperm extracts. The data suggest that human sperm extracts can activate oocytes of different phyla by the same mechanism as homologous spermatozoa. Injection of inositol 1,4,5‐trisphosphate (IP3) into C. intestinalis oocytes mimicked to some extent the initial stages of oocyte activation, but the results demonstrate that ascidian oocyte activation by human sperm extract cannot be explained solely in terms of IP3‐induced calcium release. Injection of other calcium releasing second messengers, cyclic adenosine diphosphate ribose, or calcium ions, does not lead to oocyte activation or release intracellular calcium in ascidian oocytes. It was concluded that human spermatozoa contain one or more molecules that can trigger intracellular calcium release in oocytes from different phyla.

[1]  B. Dale,et al.  Maturation promoting factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II. , 1996, Development.

[2]  D. Epel,et al.  An early increase in cGMP follows fertilization of sea urchin eggs. , 1996, Biochemical and biophysical research communications.

[3]  A. Sesay,et al.  Calcium oscillations in mammalian eggs triggered by a soluble sperm protein , 1996, Nature.

[4]  C. Sardet,et al.  Function and characteristics of repetitive calcium waves associated with meiosis , 1995, Current Biology.

[5]  S. Homa,et al.  A cytosolic sperm factor triggers calcium oscillations and membrane hyperpolarizations in human oocytes. , 1994, Human reproduction.

[6]  A. Galione,et al.  Ca2+ release induced by cyclic ADP-ribose. , 1994, Trends in cell biology.

[7]  B. Dale.  Oocyte activation in invertebrates and humans , 1994, Zygote.

[8]  K. Swann.  Ca2+ oscillations and sensitization of Ca2+ release in unfertilized mouse eggs injected with a sperm factor. , 1994, Cell calcium.

[9]  B. Dale,et al.  Regulation of the fertilization current in ascidian oocytes by intracellular second messengers , 1994, Molecular reproduction and development.

[10]  M. Osawa.  Soluble sperm extract triggers inositol 1,4,5-trisphosphate-induced Ca2+ release in the oocytes of the sea urchin, Anthocidaris crassispina. , 1994, Cell structure and function.

[11]  K. Foltz,et al.  Recepter-mediated signal transduction and egg activation , 1993, Zygote.

[12]  K. Swann.  The soluble sperm oscillogen hypothesis , 1993, Zygote.

[13]  B. Dale,et al.  Inositol tri‐phosphate in human and ascidian spermatozoa , 1993, Molecular Reproduction and Development.

[14]  K. Swann,et al.  Activation of Ca(2+)-dependent currents in cultured rat dorsal root ganglion neurones by a sperm factor and cyclic ADP-ribose. , 1992, Molecular biology of the cell.

[15]  K. Swann.  Different triggers for calcium oscillations in mouse eggs involve a ryanodine-sensitive calcium store. , 1992, The Biochemical journal.

[16]  T. Südhof,et al.  Structure of a novel InsP3 receptor. , 1991, The EMBO journal.

[17]  K. Swann,et al.  A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilization in hamster eggs. , 1990, Development.

[18]  T. Rakow,et al.  Multiple stores of calcium are released in the sea urchin egg during fertilization. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[19]  C. Sardet,et al.  Periodic calcium waves cross ascidian eggs after fertilization. , 1990, Developmental biology.

[20]  K. Iwasa,et al.  High concentration of inositol 1,4,5-trisphosphate in sea urchin sperm. , 1990, Biochemical and biophysical research communications.

[21]  C. Brownlee,et al.  Temporal and spatial correlation of fertilization current, calcium waves and cytoplasmic contraction in eggs of Ciona intestinalis , 1990, Proceedings of the Royal Society of London. B. Biological Sciences.

[22]  S. Stice,et al.  Activation of mammalian oocytes by a factor obtained from rabbit sperm , 1990, Molecular reproduction and development.

[23]  T. Südhof,et al.  Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor , 1989, Nature.

[24]  Teiichi Furuichi,et al.  Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400 , 1989, Nature.

[25]  Christopher A. Ross,et al.  Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons , 1989, Nature.

[26]  B. Dale.  Primary and secondary messengers in the activation of ascidian eggs. , 1988, Experimental cell research.

[27]  P. Berggren,et al.  Heparin inhibits inositol trisphosphate-induced calcium release from permeabilized rat liver cells. , 1987, Biochemical and biophysical research communications.

[28]  B. Dale.  Fertilization channels in ascidian eggs are not activated by Ca. , 1987, Experimental cell research.

[29]  R. Steinhardt,et al.  Ionic regulation of egg activation , 1982, Quarterly Reviews of Biophysics.

[30]  K. Swann,et al.  Lighting the fuse at fertilization , 1993 .

[31]  B. Dale,et al.  Sperm-activated channels in ascidian oocytes. , 1984, Developmental biology.