A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection

In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 × 10−6 refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL−1, compared to 1 μg mL−1 and 0.5 μg mL−1, as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection.

[1]  Marek Piliarik,et al.  Multi-analyte surface plasmon resonance biosensing. , 2005, Methods.

[2]  Chi Lok Wong,et al.  Imaging surface plasmon resonance (SPR) photonic sensors , 2007 .

[3]  F. Romanato,et al.  A surface acoustic wave (SAW)-enhanced grating-coupling phase-interrogation surface plasmon resonance (SPR) microfluidic biosensor. , 2016, Lab on a chip.

[4]  Zhiping Lin,et al.  Multiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme. , 2011, Optics express.

[5]  S. G. Nelson,et al.  High sensitivity surface plasmon resonace sensor based on phase detection , 1996 .

[6]  U. S. Dinish,et al.  Non-labeling multiplex surface enhanced Raman scattering (SERS) detection of volatile organic compounds (VOCs). , 2014, Analytica chimica acta.

[7]  Chii-Wann Lin,et al.  Improvement of the sensitivity of the surface plasmon resonance sensors based on multi-layer modulation techniques , 2015 .

[8]  Malini Olivo,et al.  Surface Plasmon Resonance Imaging Sensors: A Review , 2014, Plasmonics.

[9]  Ding Xiang,et al.  A surface plasmon resonance imaging interferometry for protein micro-array detection , 2005 .

[10]  G. Serrano-Heras,et al.  Real-time PCR detection chemistry. , 2015, Clinica chimica acta; international journal of clinical chemistry.

[11]  Gianluca Ruffato,et al.  High-throughput fabrication and calibration of compact high-sensitivity plasmonic lab-on-chip for biosensing , 2016 .

[12]  Yuan-Ting Zhang,et al.  A surface plasmon resonance system for the measurement of glucose in aqueous solution , 2005 .

[13]  Sang Yup Lee,et al.  Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein. , 2012, Talanta.

[14]  Chi Lok Wong,et al.  Recent advances in SPR and SERS for sensitive translational medical diagnostics , 2015 .

[15]  P. Shum,et al.  Optical Characterization of Elastohydrodynamic Lubrication Pressure with Surface Plasmon Resonance , 2011 .

[16]  J. Mahony Detection of Respiratory Viruses by Molecular Methods , 2008, Clinical Microbiology Reviews.

[17]  M. Hämäläinen,et al.  A novel assay for influenza virus quantification using surface plasmon resonance. , 2010, Vaccine.

[18]  Chinlon Lin,et al.  Sensitivity enhancement based on application of multi-pass interferometry in phase-sensitive surface plasmon resonance biosensor , 2007 .

[19]  H. Ho,et al.  Surface Plasmon Resonance Biosensor Incorporated in a Michelson Interferometer WithEnhanced Sensitivity , 2007, IEEE Sensors Journal.

[20]  T. Heikkinen,et al.  Feasibility of diagnosing influenza within 24 hours of symptom onset in children 1–3 years of age , 2011, European Journal of Clinical Microbiology & Infectious Diseases.

[21]  C. L. Wong,et al.  Application of surface plasmon resonance sensing to studying elastohydrodynamic lubricant films. , 2005, Applied optics.

[22]  Ho-Pui Ho,et al.  Application of differential phase measurement technique to surface plasmon resonance sensors , 2003 .

[23]  Chi Lok Wong,et al.  Surface-enhanced Raman scattering (SERS)-based volatile organic compounds (VOCs) detection using plasmonic bimetallic nanogap substrate , 2014 .

[24]  J. Homola Surface plasmon resonance based sensors , 2006 .

[25]  Chinlon Lin,et al.  Biosensor Arrays based on Surface Plasmon Resonance Phase Imaging , 2006, 2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials.

[26]  M. Bergeron,et al.  Diagnosing infections--current and anticipated technologies for point-of-care diagnostics and home-based testing. , 2010, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[27]  Günter Gauglitz,et al.  Surface plasmon resonance sensors: review , 1999 .

[28]  Chii-Wann Lin,et al.  Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies. , 2016, Journal of virological methods.

[29]  Q.L. Chen,et al.  Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging. , 2008, Biosensors & bioelectronics.

[30]  C. L. Wong,et al.  Photothermal imaging of nanoparticles beyond the diffraction limit , 2011 .

[31]  H. Ho,et al.  Application of two-dimensional spectral surface plasmon resonance to imaging of pressure distribution in elastohydrodynamic lubricant films. , 2006, Applied optics.

[32]  Chi Lok Wong,et al.  Optical characterization of elastohydrodynamic lubricated (EHL) contacts using surface plasmon resonance (SPR) effect , 2008 .

[33]  Dae-Ki Kim,et al.  Tools to Detect Influenza Virus , 2013, Yonsei medical journal.

[34]  Colorimetric surface plasmon resonance imaging (SPRI) biosensor array based on polarization orientation. , 2013, Biosensors & bioelectronics.

[35]  Application of spectral surface plasmon resonance to gas pressure sensing , 2005 .