Facile electrochemical affinity measurements of small and large molecules

A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein–protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

[1]  B. Moosmann,et al.  Development of a non-radioactive mass spectrometry-based binding assay at the μ-opioid receptor and its application for the determination of the binding affinities of 17 opiates/opioids as well as of the designer opioid isotonitazene and five further 2-benzylbenzimidazoles. , 2022, Analytica chimica acta.

[2]  Marimuthu Citartan,et al.  Assays to Estimate the Binding Affinity of Aptamers , 2022 .

[3]  C. Malitesta,et al.  A Comparison of EIS and QCM NanoMIP-Based Sensors for Morphine. , 2021, Nanomaterials.

[4]  M. Vogel,et al.  In vitro data suggest that Indian delta variant B.1.617 of SARS‐CoV‐2 escapes neutralization by both receptor affinity and immune evasion , 2021, Allergy.

[5]  R. Scheltema,et al.  The assessment of Pseudomonas aeruginosa lectin LecA binding characteristics of divalent galactosides using multiple techniques , 2021, Glycobiology.

[6]  M. Vogel,et al.  Molecular definition of SARS-CoV-2 RBD mutations: receptor affinity versus neutralization of receptor interaction. , 2021, Allergy.

[7]  Daniela F. Báez,et al.  Graphene-based sensors for small molecule determination in real samples , 2021 .

[8]  J. Lebbink,et al.  Experimental Evidence for Enhanced Receptor Binding by Rapidly Spreading SARS-CoV-2 Variants , 2021, bioRxiv.

[9]  H. Turner,et al.  Multimerization- and glycosylation-dependent receptor binding of SARS-CoV-2 spike proteins , 2020, bioRxiv.

[10]  Maria D. L. Oliveira,et al.  Lectin-based impedimetric biosensor for differentiation of pathogenic candida species. , 2020, Talanta.

[11]  K. Shi,et al.  Structural basis of receptor recognition by SARS-CoV-2 , 2020, Nature.

[12]  C. Banks,et al.  Nanomodified Screen-Printed Electrode for direct determination of Aflatoxin B1 in malted barley samples , 2020 .

[13]  Lina Baranauskienė,et al.  Binding affinity in drug design: experimental and computational techniques , 2019, Expert opinion on drug discovery.

[14]  R. Pieters,et al.  Assembly of Divalent Ligands and Their Effect on Divalent Binding to Pseudomonas aeruginosa Lectin LecA , 2019, The Journal of organic chemistry.

[15]  J. Paulson,et al.  Kinetic analysis of the influenza A virus HA/NA balance reveals contribution of NA to virus-receptor binding and NA-dependent rolling on receptor-containing surfaces , 2018, PLoS pathogens.

[16]  J. Hart,et al.  Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode. , 2018, Food chemistry.

[17]  A. Stocker,et al.  Structural Insight into Multivalent Galactoside Binding to Pseudomonas aeruginosa Lectin LecA. , 2015, ACS chemical biology.

[18]  Wendong Zhang,et al.  Progress of new label-free techniques for biosensors: a review , 2015, Critical reviews in biotechnology.

[19]  B. Youan,et al.  Concanavalin A-polysaccharides binding affinity analysis using a quartz crystal microbalance. , 2014, Biosensors & bioelectronics.

[20]  A. Stocker,et al.  A Glycopeptide Dendrimer Inhibitor of the Galactose-Specific Lectin LecA and of Pseudomonas aeruginosa Biofilms , 2011, Angewandte Chemie.

[21]  Tanin Tangkuaram,et al.  Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan. , 2007, Biosensors & bioelectronics.

[22]  Laura L Kiessling,et al.  Surface plasmon resonance imaging studies of protein-carbohydrate interactions. , 2003, Journal of the American Chemical Society.

[23]  Jian Qin.  Investigating the Effects of Two Different Carbon Materials on the Sensitivity of an Electrochemical Impedimetric Lectin-Based Biosensor , 2020 .

[24]  Martin Pumera,et al.  Electrochemical nanobiosensors , 2007 .