A method for determining optical proline concentrations in samples of natural waters, based on the measurement value angle of the plane of polarization of radiation of a certain wavelength λ, which has been studied by the liquid thickness l = 5 sm was developed. Three wavelength λ = 400, 450, 500 nm were used. A series of reference samples was produced: aqueous solutions of L and D forms proline of a known concentration within c = (0.001 - 0,1) %. Based on these studies, for each λ gauge dependent quantities of angle α = f (c) was built. Using them and conducting measurements of angles α for the real natural environments, the possibility of determination of the concentration of proline in them was actualized. It was determined that in waters within the city of Chernivtsy and its surroundings occured proline of two forms: L and D, the concentration of which is within c = (0,005 - 0,094)%.
[1]
Wu Yu.
Optical Circular Dichroism of Deoxoqinghaosu and Qinghaosu
,
1991
.
[2]
Sofia Simmonds,et al.
Biochemistry of the Amino Acids
,
1966,
Nature.
[3]
H. Jakubke,et al.
Amino Acids, Peptides and Proteins
,
1977
.
[4]
Emil Wolf,et al.
Principles of Optics: Contents
,
1999
.
[5]
Kazuma Noguchi,et al.
The presence of high concentrations of free D-amino acids in human saliva.
,
2006,
Life sciences.
[6]
W. Schwan,et al.
Low-proline environments impair growth, proline transport and in vivo survival of Staphylococcus aureus strain-specific putP mutants.
,
2004,
Microbiology.
[7]
I. D. Teare,et al.
Rapid determination of free proline for water-stress studies
,
1973,
Plant and Soil.