Immunocharacterization of Taenia solium oncosphere and metacestode antigens

Abstract A partial immunocharacterization of oncosphere and metacestode antigens of Taenia solium was carried out and compared to antigens from other taeniid species. The results indicated that T. solium metacestode antigen contained epitopes cross reactive with rabbit anti-sera to adult and oncospheral stages of the parasite. Oncospheres, however, consisted largely of stage specific antigens. Western blot analysis indicated that T. solium and T. pisiformis shared several oncospheral antigens; however, this was not the case with T. solium and T. hydatigena. Western blot analysis showed a time-related increase in the number of molecules recognized by antibodies to T. solium oncosphere and metacestode antigens in pigs experimentally infected with T. solium eggs. Oncosphere specific antibodies were detected in pig sera one month after experimental infection whereas antibodies to cystic stage antigens were not present until the 3rd to 5th month post infection. Sera from neurocysticercotic patients as well as naturally infected cysticercotic pigs recognized high molecular weight antigens in the oncospheres.

[1]  D. D. Heath,et al.  Taenia ovis recombinant vaccine – ‘quo vadit’ , 1995, Parasitology.

[2]  W. Shandera,et al.  Neurocysticercosis in Houston, Texas: A Report of 112 Cases , 1994, Medicine.

[3]  C. Shoemaker,et al.  cDNA cloning and recombinant expression of collagen-binding and complement inhibitor activity of Taenia solium paramyosin (AgB). , 1993, Molecular and biochemical parasitology.

[4]  S. Waterman,et al.  Cysticercosis surveillance: locally acquired and travel-related infections and detection of intestinal tapeworm carriers in Los Angeles County. , 1992, The American journal of tropical medicine and hygiene.

[5]  P. Craig,et al.  Salivary and serum antibodies in experimental canine taeniasis. , 1992, Veterinary parasitology.

[6]  C. Shoemaker,et al.  Paramyosin inhibits complement C1. , 1992, Journal of immunology.

[7]  D. Packham,et al.  Five cases of neurocysticercosis diagnosed in Sydney. , 1991, The Southeast Asian journal of tropical medicine and public health.

[8]  H. Bøgh,et al.  Molecular cloning of Taenia taeniaeformis oncosphere antigen genes. , 1991, Molecular and biochemical parasitology.

[9]  C. Shoemaker,et al.  Paramyosin is the Schistosoma mansoni (Trematoda) homologue of antigen B from Taenia solium (Cestoda). , 1991, Molecular and biochemical parasitology.

[10]  K. Onitake,et al.  Vaccination against Taenia taeniaeformis infection in rats using a recombinant protein and preliminary analysis of the induced antibody response. , 1991, Molecular and biochemical parasitology.

[11]  M. Brandon,et al.  Cysticercosis: cellular immune responses during primary and secondary infection , 1990, Parasite immunology.

[12]  G. Mitchell,et al.  Stage‐specific immunity to Taenia taeniaeformis infection in mice. A histological study of the course of infection in mice vaccinated with either oncosphere or metacestode antigens , 1990, Parasite immunology.

[13]  K. Pathak,et al.  Immunization of pigs with culture antigens of Taenia solium. , 1990, Veterinary parasitology.

[14]  M. Sewell,et al.  Developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable Taenia saginata larvae , 1989, Parasitology.

[15]  J. Gathuma,et al.  Specific and shared antigenic components of Taenia saginata oncospheres. , 1989, Research in veterinary science.

[16]  K. O'Hoy,et al.  Vaccination against ovine cysticercosis using a defined recombinant antigen , 1989, Nature.

[17]  Harris Lj,et al.  Taenia saginata and Taenia solium: reciprocal models. , 1989 .

[18]  J. Laclette,et al.  The coexistence of Taenia solium cysticerci and the pig: role of the antigen B. , 1989, Acta Leidensia.

[19]  R. Parkhouse,et al.  Taenia saginata and Taenia solium: reciprocal models. , 1989, Acta Leidensia.

[20]  A. Flisser,et al.  Swine cysticercosis treated with praziquantel, a short report. , 1989, Acta Leidensia.

[21]  M. Lightowlers.  Recent advances in vaccination against cysticercosis. , 1989, Acta Leidensia.

[22]  P. Tato,et al.  Inmunity in mice to an oncosphere infection by using oncospheral antigens from Taenia solium or Taeniarhynchus saginatus. , 1988, Revista latinoamericana de microbiologia.

[23]  M. Lightowlers,et al.  Studies on stage‐specific immunity against Taenia Taeniaeformis metacestodes in mice , 1988, Parasite immunology.

[24]  A. Flisser,et al.  Neurocysticercosis in Mexico. , 1988, Parasitology today.

[25]  P. Craig,et al.  Immunization against experimental rabbit cysticercosis using liposome-associated antigen preparations , 1988, Journal of Helminthology.

[26]  C. Filley,et al.  Neurocysticercosis in the United States: 35 cases and a review. , 1987, Reviews of infectious diseases.

[27]  J. Laclette,et al.  Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium. , 1987, The Journal of parasitology.

[28]  A. Flisser,et al.  Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis , 1986, Journal of clinical microbiology.

[29]  E. Ruiz-Tiben,et al.  Cysticercosis in Los Angeles County. , 1985, JAMA.

[30]  P. Tato,et al.  Taenia solium: cell reactions to the larva (Cysticercus cellulosae) in naturally parasitized, immunized hogs. , 1983, Experimental parasitology.

[31]  P. Stevenson.  Observations on the hatching and activation of fresh Taenia saginata eggs. , 1983, Annals of tropical medicine and parasitology.

[32]  P. Tato,et al.  Taenia solium: immunity in hogs to the Cysticercus. , 1983, Experimental parasitology.

[33]  A. Flisser,et al.  Cysticercosis: present state of knowledge and perspectives. , 1982 .

[34]  J. F. Williams,et al.  Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. , 1982, Advances in parasitology.

[35]  Princes Highway.  Anti-Oneospheral Antibodies in the Serum of Lambs Experimentally Infected with Either Taenia ovis or Taenia hydatigena , 1981 .

[36]  G. Mitchell,et al.  Immunization of mice against infection with Taenia taeniaeformis using various antigens prepared from eggs, oncospheres, developing larvae and strobilocerci. , 1980, International journal for parasitology.

[37]  G. Mitchell,et al.  Density-gradient separation of Taenia pisiformis oncospheres. , 1980, The Journal of parasitology.

[38]  A. Flisser,et al.  Human cysticercosis: antigens, antibodies and non-responders. , 1980, Clinical and experimental immunology.

[39]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[40]  M. Rickard,et al.  Vaccination of lambs against infection with Taenia ovis using antigens collected during short-term in vitro incubation of activated T. ovis oncospheres , 1977, Parasitology.

[41]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[42]  D. D. Heath.  Resistance to Taenia pisiformis larvae in rabbits: immunization against infection using non-living antigens from in vitro culture. , 1976, International journal for parasitology.

[43]  E. Weiss.  In Vitro Cultivation , 1971 .

[44]  J. D. Smyth,et al.  In vitro cultivation of Echinococcus granulosus, Taenia hydatigena, T. ovis, T. pisiformis and T. serialis from oncosphere to cystic larva , 1970, Parasitology.

[45]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.