Ultrasound measures of muscle thickness: intra‐examiner reliability and influence of body position

Ultrasound is an inexpensive and practical alternative to gold standard measures of muscle mass. Changes in body position may lead to intra‐muscular fluid shifts that may affect the reliability of ultrasound measures. We assessed test–retest reliability of ultrasound measures of muscle thickness and the effect of position on these measures. Measures of muscle thickness were made from B‐mode ultrasound images in 18 healthy participants. Repeated measurements were made by one examiner from two participant positions; standing and recumbent, from 18 anatomical sites. Results demonstrated high test–retest reliability for measures taken in both participant positions [intra‐class coefficient (ICC) scores 0·65 to 0·94], except for the recumbent posterior lower leg measures (ICC 0·34). Recumbent measures were significantly smaller than those taken with participants standing. Length of time participants spent lying down did not significantly affect measures, indicating that any changes in intra‐muscular fluid related to the time spent recumbent are negligible.

[1]  J. R. Landis,et al.  The measurement of observer agreement for categorical data. , 1977, Biometrics.

[2]  Takashi Abe,et al.  Prediction and validation of total and regional skeletal muscle mass by ultrasound in Japanese adults , 2005, European Journal of Applied Physiology.

[3]  R. Lindle,et al.  Effects of acute supine rest on mid‐thigh cross‐sectional area as measured by computed tomography , 2007, Clinical physiology and functional imaging.

[4]  Validity of Methods Used for the Assessment of Body Components in Children , 2007 .

[5]  Robert Topp,et al.  The effect of bed rest and potential of prehabilitation on patients in the intensive care unit. , 2002, AACN clinical issues.

[6]  S. Kritchevsky,et al.  Strength and Muscle Quality in a Well‐Functioning Cohort of Older Adults: The Health, Aging and Body Composition Study , 2003, Journal of the American Geriatrics Society.

[7]  C. Reimers,et al.  Age-related muscle atrophy does not affect all muscles and can partly be compensated by physical activity: An ultrasound study 1 Presented in part at the 34th German Congress of Sports Medicine in Saarbrücken/Germany, October 19–22, 1995 [8]. 1 , 1998, Journal of the Neurological Sciences.

[8]  J. Wells.  Validity of Methods Used for the Assessment of Body Components in Children: Pros and Cons of Modern versus Old Technology , 2007, Hormone Research in Paediatrics.

[9]  R. Macko,et al.  Cardiovascular Health and Fitness After Stroke , 2005, Topics in stroke rehabilitation.

[10]  M. Siimes,et al.  Quantitation of Muscles and Fat by Ultrasonography: A Useful Method in the Assessment of Malnutrition in Children , 1991, Acta paediatrica Scandinavica.

[11]  P A Tesch,et al.  Changes in lower limb muscle cross-sectional area and tissue fluid volume after transition from standing to supine. , 1993, Acta physiologica Scandinavica.

[12]  Raul N Uppot,et al.  Impact of obesity on medical imaging and image-guided intervention. , 2007, AJR. American journal of roentgenology.

[13]  T. Fukunaga,et al.  Prediction equations for body composition of Japanese adults by B‐mode ultrasound , 1994, American journal of human biology : the official journal of the Human Biology Council.