Selective retrograde labeling indicating the transmitter of neuronal pathways

A number of tritiated transmitter related compounds—amino acids and biogenic amines—were injected into the rat caudoputamen or substantia nigra in order to test (1) for the occurence of autoradiographic perikaryal labeling, (2) for a selectivity of perikaryal labeling relating certain compounds to certain pathways, and (3) for the relation of perikaryal labeling to known transmitter specificities of the systems involved. Perikaryal labeling was observed after injection of some but not all of the substances used and was best explained by retrograde labeling in pathways projecting to the injection sites. Six hours after injection of high concentrations of tritiated transmitter into the terminal area, perikaryal labeling was observed: (A) in substantial nigra compacta (A9), A10 (rostral) and A8 (all heavy), and in n. raphe dorsalis (light) after [3H]‐dopamine and [3H]‐norepinephrine injection into caudoputamen; (B) same pattern as in A, but heavy in n. raphe dorsalis after [3H]‐serotonin injection into caudoputamen; perikaryal labeling absent in cortex and thalamus after injection of substances mentioned in A and B; (C) only in substantia nigra compacta (minimally) after [3H]‐GABA injection into caudoputamen; (D) in cerebral cortex and thalamus but not in substantia nigra, A10, A8, nor in n. raphe dorsalis after injection of [3H]‐D‐aspartate into caudoputamen; (E) in the rat caudoputamen but not in n. raphe dorsalis after [3H]‐GABA injection into substantia nigra; (F) in n. raphe dorsalis but not in caudoputamen after [3H]‐serotonin into substantia nigra.

[1]  M. Cuénod,et al.  Transmitter-related retrograde labeling in the pigeon optic lobe; a high resolution autoradiographic study , 1980, Brain Research.

[2]  M. Cuénod,et al.  Glutamate release in vitro from corticostriatal terminals , 1979, Brain Research.

[3]  M. Cuénod,et al.  Transmitter-specific retrograde labeling in the striato-nigral and raphe-nigral pathways. , 1979, Science.

[4]  J. K. Saelens,et al.  Further evidence for cholinergic thalamo‐striatal neurons , 1979, Journal of neurochemistry.

[5]  F. Fonnum,et al.  The effects of surgical and chemical lesions on neurotransmitter candidates in the nucleus accumbens of the rat , 1979, Neuroscience.

[6]  H. Fibiger,et al.  Anterior striatal projections to the globus pallidus, entopeduncular nucleus and substantia nigra in the rat: The GABA connection , 1978, Brain Research.

[7]  R. Faull,et al.  The cells of origin of nigrotectal, nigrothalamic and nigrostriatal projections in the rat , 1978, Neuroscience.

[8]  M. Cuénod,et al.  Glutamine as precursor for the GABA and glutamate trasmitter pools , 1978, Neuroscience Letters.

[9]  P. Emson,et al.  Topographic projections of substance P and GABA pathways in the striato- and pallido-nigral system: A biochemical and immunohistochemical study , 1978, Brain Research.

[10]  Y. Agid,et al.  Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer in the rat brain hippocampal system , 1978, Brain Research.

[11]  P. Tongroach,et al.  The dorsal and medial raphe projections to the substantia nigra in the rat: Electrophysiological, biochemical and behavioural observations , 1978, Brain Research.

[12]  R. Moore,et al.  Serotonin neurons of the midbrain raphe: Ascending projections , 1978, The Journal of comparative neurology.

[13]  J. Fallon,et al.  Catecholamine innervation of the basal forebrain IV. Topography of the dopamine projection to the basal forebrain and neostriatum , 1978, The Journal of comparative neurology.

[14]  B. Berger,et al.  Dopamine uptake in serotoninergic terminals in vitro: A valuable tool for the histochemical differentiation of catecholaminergic and serotoninergic terminals in rat cerebral structures , 1978, Brain Research.

[15]  F. Bloom,et al.  Differential projections of neurons within the dorsal raphe nucleus of the rat: a horseradish peroxidase (HRP) study , 1978, Brain Research.

[16]  G. P. Smith,et al.  Efferent connections and nigral afferents of the nucleus accumbens septi in the rat , 1978, Neuroscience.

[17]  Anders Björklund,et al.  Organization of catecholamine neurons projecting to the frontal cortex in the rat , 1978, Brain Research.

[18]  M. Mesulam,et al.  Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[19]  H. Vanegas,et al.  Early stages of uptake and transport of horseradish‐peroxidase by cortical structures, and its use for the study of local neurons and their processes , 1978, The Journal of comparative neurology.

[20]  J. Reubi,et al.  Release and distribution of endogenous 5-HT in rat substantia nigra , 1978, Brain Research.

[21]  H. Fibiger,et al.  An anatomical and electrophysiological investigation of the serotonergic projection from the dorsal raphe nucleus to the substantia nigra in the rat , 1977, Neuroscience.

[22]  M. Brownstein,et al.  On the origin of substance P and glutamic acid decarboxylase (GAD) in the substantia nigra , 1977, Brain Research.

[23]  S. Wise,et al.  Cells of origin and terminal distribution of descending projections of the rat somatic sensory cortex , 1977, The Journal of comparative neurology.

[24]  R. Hassler,et al.  Effect of frontal cortex ablation on striatal glutamic acid level in rat , 1977, Brain Research.

[25]  S. Hunt,et al.  Characterization of the pigeon isthmo-tectal pathway by selective uptake and retrograde movement of radioactive compounds and by golgi-like horseradish peroxidase labeling , 1977, Brain Research.

[26]  P. Mcgeer,et al.  A glutamatergic corticostriatal path? , 1977, Brain Research.

[27]  Barbara E. Jones,et al.  Ascending projections of the locus coeruleus in the rat. II. Autoradiographic study , 1977, Brain Research.

[28]  J. K. Saelens,et al.  Evidence for a cholinergic fiber tract connecting the thalamus with the head of the striatum of the rat , 1977, Brain Research.

[29]  H. Thoenen,et al.  Retrograde axonal transport of125I-tetanus toxin as a too for tracing fiber connections in the central nervous system; connections of the rostral part of the rat neostriatum , 1977, Brain Research.

[30]  M. Brownstein,et al.  Evidence for substance P in the striato-nigral tract , 1977, Brain Research.

[31]  I. Divac,et al.  High affinity uptake of glutamate in terminals of corticostriatal axons , 1977, Nature.

[32]  G. Racagni,et al.  Projections of substance P containing neurons from neostriatum to substantia nigra , 1977, Brain Research.

[33]  I. Kanazawa,et al.  Evidence for the existence of substance P-containing fibres in striato-nigral and pallido-nigral pathways in rat brain , 1977, Brain Research.

[34]  B. Bunney,et al.  The precise localization of nigral afferents in the rat as determined by a retrograde tracing technique , 1976, Brain Research.

[35]  H. Künzle,et al.  Selective uptake and transport of label within three identified neuronal systems after injection of 3H‐GABA into the pigeon optic tectum: An autoradiographic and golgi study , 1976, The Journal of comparative neurology.

[36]  M. Palkovits,et al.  Regional distribution of substance P in the brain of the rat , 1976, Brain Research.

[37]  L. Iversen,et al.  Release and metabolism of substance P in rat hypothalamus , 1976, Nature.

[38]  George R. Marshall,et al.  Afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus , 1976, Brain Research.

[39]  K. Kristensson,et al.  Retrograde transport of horseradish peroxidase in transected axons. 3. Entry into injured axons and subsequent localization in perikaryon , 1976, Brain Research.

[40]  M. Carpenter,et al.  Projections of the globus pallidus and adjacent structures: An autoradiographic study in the monkey , 1976, The Journal of comparative neurology.

[41]  Michel Jouvet,et al.  The raphe nuclei of the cat brain stem: A topographical atlas of their efferent projections as revealed by autoradiography , 1976, Brain Research.

[42]  D. Pfaff,et al.  Autoradiographic tracing of nucleus accumbens efferents in the rat , 1976, Brain Research.

[43]  A. Dray,et al.  Evidence for the existence of a raphe projection to the substantia nigra in rat , 1976, Brain Research.

[44]  M. Cuénod,et al.  Release of exogenous glycine in the pigeon optic tectum during stimulation of a midbrain nucleus , 1976, Brain Research.

[45]  L. P. Davies,et al.  UPTAKE AND RELEASE OF D‐ AND L‐ASPARTATE BY RAT BRAIN SLICES , 1976, Journal of neurochemistry.

[46]  W. Foote,et al.  The efferent connection of the nucleus raphe dorsalis , 1976, Brain Research.

[47]  E. W. Powell,et al.  Connections of the nucleus accumbens , 1976, Brain Research.

[48]  F. Reinoso-suárez,et al.  Projections to gyrus sigmoideus from the substantia nigra in the cat, as revealed by the horseradish peroxidase retrograde transport technique , 1976, Neuroscience Letters.

[49]  Hugh J. Spencer Antagonism of cortical excitation of striatal neurons by glutamic acid diethyl ester: Evidence for glutamic acid as an excitatory transmitter in the rat striatum , 1976, Brain Research.

[50]  H. Fibiger,et al.  Anatomical and electrophysiological identification of a projection from the mesencephalic raphe to the caudate-putamen in the rat , 1975, Brain Research.

[51]  H. Fibiger,et al.  Demonstration of a pallido‐nigral projection innervating dopaminergic neurons , 1975, The Journal of comparative neurology.

[52]  W. Cowan,et al.  A note on the connections and development of the nucleus accumbens , 1975, Brain Research.

[53]  I. Grofová The identification of striatal and pallidal neurons projecting to substantia nigra An experimental study by means of retrograde axonal transport of horseradish peroxidase , 1975, Brain Research.

[54]  J. Kelly,et al.  Selective uptake of [3H]β-alanine by glia: Association with the glial uptake system for GABA , 1975, Brain Research.

[55]  D. Salvert,et al.  Differential projections of the nucleus raphe dorsalis and nucleus raphe centralis as revealed by autoradiography , 1975, Brain Research.

[56]  J. Kelly,et al.  The autoradiographic localization of the gaba-releasing nerve terminals in cerebellar glomeruli , 1975, Brain Research.

[57]  T. Ho¨kfelt,et al.  Retrograde peroxidase tracing of neurons combined with transmitter histochemistry , 1975, Brain Research.

[58]  K. Kristensson,et al.  Retrograde transport of horseradish peroxidase in transected axons. 1. Time relationships between transport and induction of chromatolysis. , 1974, Brain research.

[59]  T. Maeda,et al.  Detailed noradrenaline pathways of locus coeruleus neuron to the cerebral cortex with use of 6-hydroxydopa. , 1974, Brain research.

[60]  S. Lorens,et al.  Regional 5-hydroxytryptamine following selective midbrain raphe lesions in the rat. , 1974, Brain research.

[61]  K. Stöckel,et al.  Specificity of the retrograde axonal transport of nerve growth factor. , 1974, Brain research.

[62]  F. Fonnum,et al.  Origin and distribution of glutamate decarboxylase in substantia nigra of the cat. , 1974, Brain research.

[63]  E. Jones,et al.  Retrograde axonal transport and the demonstration of non‐specific projections to the cerebral cortex and striatum from thalamic intralaminar nuclei in the rat, cat and monkey , 1974, The Journal of comparative neurology.

[64]  H. Nauta,et al.  Afferents to the rat caudoputamen studied with horseradish peroxidase. An evaluation of a retrograde neuroanatomical research method. , 1974, Brain research.

[65]  H. Kuypers,et al.  Retrograde axonal transport of horseradish peroxidase in rats forebrain. , 1974, Brain research.

[66]  K. Fuxe,et al.  Mapping of central noradrenaline pathways with 6-hydroxy-DOPA. , 1973, Brain research.

[67]  T. Hökfelt,et al.  Specificity of 6-hydroxydopamine induced degeneration of central monoamine neurones: an electron and fluorescence microscopic study with special reference to intracerebral injection on the nigro-striatal dopamine system. , 1973, Brain research.

[68]  D. L. Martin,et al.  The effects of L-2,4-diaminobutyric acid on the uptake of gamma-aminobutyric acid by a synaptosomal fraction from rat brain. , 1973, Archives of biochemistry and biophysics.

[69]  H. Fibiger,et al.  On the source of GABA-containing terminals in the substantia nigra. Electron microscopic autoradiographic and biochemical studies. , 1973, Brain research.

[70]  V. Balcar,et al.  THE STRUCTURAL SPECIFICITY OF THE HIGH AFFINITY UPTAKE OF l‐GLUTAMATE AND l‐ASPARTATE BY RAT BRAIN SLICES , 1972, Journal of neurochemistry.

[71]  R. Roth,et al.  Tryptophan hydroxylase activity and synaptosomal uptake of serotonin in discrete brain regions after midbrain raphe lesions: correlations with serotonin levels and histochemical fluorescence. , 1972, Brain research.

[72]  U. Ungerstedt Stereotaxic mapping of the monoamine pathways in the rat brain. , 1971, Acta physiologica Scandinavica. Supplementum.

[73]  M. Perenin,et al.  Lésions internucléaires: effets sur la motricitéoculaire pendant l'éveil et le sommeil paradoxal chez le chat , 1971 .

[74]  S. Snyder,et al.  Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. , 1970, The Journal of pharmacology and experimental therapeutics.

[75]  H. Holländer The section embedding (SE) technique. A new method for the combined light microscopic and electron microscopic examination of central nervous tissue. , 1970, Brain research.

[76]  C J CLEMEDSON,et al.  DYNAMIC RESPONSE OF CHEST WALL AND LUNG INJURIES IN RABBITS EXPOSED TO AIR SHOCK WAVES OF SHORT DURATION. , 1964, Acta physiologica Scandinavica. Supplementum.

[77]  K. Fuxe,et al.  DEMONSTRATION AND MAPPING OUT OF NIGRO-NEOSTRIATAL DOPAMINE NEURONS. , 1964, Life sciences.

[78]  J. D. Robertson,et al.  THE ULTRASTRUCTURE OF MAUTHNER CELL SYNAPSES AND NODES IN GOLDFISH BRAINS , 1963, The Journal of cell biology.

[79]  F. Walberg,et al.  The raphe nuclei of the brain stem in the cat. II. Efferent connections , 1960 .

[80]  F. Fonnum,et al.  Localization of neurotransmitters, particularly glutamate, in hippocampus, septum, nucleus accumbens and superior colliculus. , 1979, Progress in brain research.

[81]  B. K. Hartman,et al.  Recent advances in neuroanatomical methodology. , 1978, Annual review of neuroscience.

[82]  A. Hamberger,et al.  Glutamate as a CNS Neurotransmitter: Properties of Release, Inactivation and Biosynthesis , 1978 .

[83]  A. Björklund,et al.  Organization of Catecholamine Neurons in the Rat Central Nervous System , 1978 .

[84]  J. Belleroche,et al.  On the Metabolic and Intrasynaptic Origin of Amino Acid Transmitters , 1978 .

[85]  F E Bloom,et al.  Central catecholamine neuron systems: anatomy and physiology of the dopamine systems. , 1978, Annual review of neuroscience.

[86]  D. Garfinkel,et al.  A Model of Glutamate Metabolism in Brain: A Biochemical Analysis of a Heterogeneous Structure , 1975 .

[87]  L. Iversen,et al.  Uptake and Localization of Transmitter Amino Acids in the Nervous System , 1975 .

[88]  S. Snyder,et al.  Neuronal Uptake of Neurotransmitters and their Precursors: Studies with “Transmitter” Amino Acids and Choline , 1973 .

[89]  S. Snyder,et al.  Uptake and Subcellular Localization of Neurotransmitters in the Brain , 1970 .

[90]  K. Fuxe,et al.  EVIDENCE FOR THE EXISTENCE OF MONOAMINE-CONTAINING NEURONS IN THE CENTRAL NERVOUS SYSTEM. I. DEMONSTRATION OF MONOAMINES IN THE CELL BODIES OF BRAIN STEM NEURONS. , 1964, Acta physiologica Scandinavica. Supplementum.

[91]  K. E. Webster Cortico-striate interrelations in the albino rat. , 1961, Journal of Anatomy.

[92]  K. C. Richardson,et al.  Embedding in epoxy resins for ultrathin sectioning in electron microscopy. , 1960, Stain technology.

[93]  J.de Groot,et al.  The rat forebrain in stereotaxic coordinates , 1959 .