Antibody to Soluble 1,3/1,6-β-D-Glucan, SCG in Sera of Naive DBA/2 Mice

A branched β-glucan from Sparassis crispa (SCG) is a major 6-branched 1,3-β-D-glucan showing antitumor activity. In the present study, we examined the anti-SCG antibody in naive mice by ELISA. Using SCG coated plate, sera of naive DBA/1 and DBA/2 mice contained significantly higher titers of antibody than other strains of mice. Anti-SCG Ab titers of each DBA/1 and DBA/2 mice were significantly varied. Using various polysaccharide-coated plate, sera of DBA/2 mice also reacted with a β-glucan from Candida spp. (CSBG) having 1,3-β and 1,6-β-glucosidic linkages. The SCG specific immunoglobulin (Ig) M but G was detected in sera. The reactivity of sera to coated SCG was neutralized by adding soluble SCG and CSBG as competitor. These results suggested that DBA/1 and DBA/2 strains carry specific and unique immunological characteristics to branched 1,3-/1,6-β-glucan.

[1]  Y. Adachi,et al.  Solubilized candida cell Wall β‐glucan, CSBG, is an epitope of natural human antibody , 2003 .

[2]  Y. Adachi,et al.  IFN-γ Induction by SCG, 1,3-β-D-Glucan from Sparassis crispa, in DBA/2 Mice In Vitro , 2002 .

[3]  Y. Adachi,et al.  Effect of SCG, 1,3-beta-D-glucan from Sparassis crispa on the hematopoietic response in cyclophosphamide induced leukopenic mice. , 2002, Biological & pharmaceutical bulletin.

[4]  S. Wasser,et al.  Hypoglycemic, Interferonogenous, and Immunomodulatory Activity of Tremellastin from the Submerged Culture of Tremella mesenterica Retz.: Fr. (Heterobasidiomycetes) , 2002 .

[5]  N. Ohno,et al.  Antitumor 1,3-β-Glucan from Cultured Fruit Body of Sparassis crispa , 2000 .

[6]  Y. Adachi,et al.  Sulubilized Cell Wall β-Glucan, CSBG, Is an Epitope of Candida Immune Mice , 2000 .

[7]  T. Yadomae.  真菌β-1,3-グルカンの構造と生物活性 , 2000 .

[8]  Y. Adachi,et al.  Solubilization of yeast cell-wall beta-(1-->3)-D-glucan by sodium hypochlorite oxidation and dimethyl sulfoxide extraction. , 1999, Carbohydrate research.

[9]  Y. Adachi,et al.  Nitric Oxide Synthesis in Murine Peritoneal Macrophages by Fungal β-Glucans , 1997 .

[10]  Y. Adachi,et al.  Effect of beta-glucans on the nitric oxide synthesis by peritoneal macrophage in mice. , 1996, Biological & pharmaceutical bulletin.

[11]  Y. Adachi,et al.  Structure-activity relationship of (1-->3)-beta-D-glucans in the induction of cytokine production from macrophages, in vitro. , 1995, Biological & pharmaceutical bulletin.

[12]  Y. Adachi,et al.  Enhancement of cytokine production by macrophages stimulated with (1-->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa. , 1994, Biological & pharmaceutical bulletin.

[13]  Y. Adachi,et al.  Preparation and Antigen Specificity of an Anti-(1→3)-β-D-glucan Antibody , 1994 .

[14]  Y. Adachi,et al.  Characterization of the antitumor glucan obtained from liquid-cultured Grifola frondosa. , 1986, Chemical & pharmaceutical bulletin.

[15]  T. Taguchi,et al.  Clinical efficacy of lentinan on neoplastic diseases. , 1983, Advances in experimental medicine and biology.