Monoclonal antibody–europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader. Quantitative detection of T-2 toxin was realized by recording fluorescence intensities of the mAb–Eu(III) probes captured on the test line (T line) and control line (C line) of the assay strips. Quantitative standard curves for determining T-2 toxin in rice, maize, and feed were established by plotting T/C ratio against the logarithmic concentration of T-2 toxin with the linear range of 0.125–200 ng g−1 for rice and maize and 0.25–200 ng g−1 for feed. The limit of detection (LOD) was 0.09 ng g−1 for rice and maize and 0.17 ng g−1 for feed. The recovery of the standard spike ranged from 94.2% to 111.0%, and the coefficient of variation (CV) was less than 15%. The results obtained by LF-TRFIA within 15 minutes showed a good correlation with the LC-MS/MS results, indicating that the LF-TRFIA method was reliable and suitable for rapid testing.

[1]  S. Ferrone,et al.  Re.: Purification of IgG monoclonal antibody by caprylic acid precipitation. , 1983, Journal of immunological methods.

[2]  R. Giese,et al.  Chromatographic enzyme immunoassay for T-2 toxin. , 1990, Journal of immunological methods.

[3]  K. Matsumoto,et al.  Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay. , 2001, Analytical chemistry.

[4]  Arindam Pal,et al.  An analytical device for on-site immunoassay. Demonstration of its applicability in semiquantitative detection of aflatoxin B1 in a batch of samples with ultrahigh sensitivity. , 2004, Analytical chemistry.

[5]  G. Eriksen,et al.  Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy metabolites. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[6]  J. Bünzli,et al.  Taking advantage of luminescent lanthanide ions. , 2005, Chemical Society reviews.

[7]  Miriam M. Ngundi,et al.  Array biosensor for detection of ochratoxin A in cereals and beverages. , 2005, Analytical chemistry.

[8]  G. Besra,et al.  Production and characterization of monoclonal antibodies against complexes of the NKT cell ligand alpha-galactosylceramide bound to mouse CD1d. , 2007, Journal of immunological methods.

[9]  R. Nitschke,et al.  Quantum dots versus organic dyes as fluorescent labels , 2008, Nature Methods.

[10]  Chris M Maragos,et al.  Improvement of detection sensitivity of T-2 and HT-2 toxins using different fluorescent labeling reagents by high-performance liquid chromatography. , 2008, Talanta.

[11]  F. Caloni,et al.  Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells. , 2009, Toxicon : official journal of the International Society on Toxinology.

[12]  R. Krska,et al.  A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food. , 2010, Talanta.

[13]  D. Milićević,et al.  Real and Perceived Risks for Mycotoxin Contamination in Foods and Feeds: Challenges for Food Safety Control , 2010, Toxins.

[14]  N. Hildebrandt,et al.  A homogeneous time-resolved fluoroimmunoassay (TR-FIA) using antibody mediated luminescence quenching , 2010 .

[15]  Suxia Zhang,et al.  T-2 toxin, a trichothecene mycotoxin: review of toxicity, metabolism, and analytical methods. , 2011, Journal of agricultural and food chemistry.

[16]  Daohong Zhang,et al.  A high selective immunochromatographic assay for rapid detection of aflatoxin B₁. , 2011, Talanta.

[17]  Yingsong Wu,et al.  Development of The Dual-labeling Time-resolved Fluoroimmunoassay for Detection of C-peptide and Insulin and Its Initially Application*: Development of The Dual-labeling Time-resolved Fluoroimmunoassay for Detection of C-peptide and Insulin and Its Initially Application* , 2011 .

[18]  W. Marsden.  I and J , 2012 .

[19]  Zhaowei Zhang,et al.  Current development of microfluidic immunosensing approaches for mycotoxin detection via capillary electromigration and lateral flow technology , 2012, Electrophoresis.

[20]  A. Visconti,et al.  Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection. , 2012, Talanta.

[21]  Yingguo Li,et al.  Development and application of a method for the analysis of two trichothecenes: deoxynivalenol and T-2 toxin in meat in China by HPLC-MS/MS. , 2012, Meat science.

[22]  V. Lattanzio,et al.  Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals. , 2012, Analytica chimica acta.

[23]  Tero Soukka,et al.  Performance of fluorescent europium(III) nanoparticles and colloidal gold reporters in lateral flow bioaffinity assay. , 2012, Analytical biochemistry.

[24]  P. L. Lakshmana Rao,et al.  T-2 toxin induced skin inflammation and cutaneous injury in mice. , 2012, Toxicology.

[25]  Qi Zhang,et al.  Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects , 2012, Sensors.

[26]  Yueming Jiang,et al.  Development of a sensitive time-resolved fluoroimmunoassay for organophosphorus pesticides in environmental water samples , 2012 .

[27]  Meihua Yang,et al.  Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization. , 2012, Food chemistry.

[28]  Zhen-jiang Liu,et al.  Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples , 2013 .

[29]  W. Shier,et al.  Cytotoxicity and phytotoxicity of trichothecene mycotoxins produced by Fusarium spp. , 2013, Toxicon : official journal of the International Society on Toxinology.

[30]  Suqing Zhao,et al.  A direct competitive inhibition time-resolved fluoroimmunoassay for the detection of unconjugated estriol in serum of pregnant women , 2013 .

[31]  Zhaowei Zhang,et al.  Advanced hyphenated chromatographic-mass spectrometry in mycotoxin determination: current status and prospects. , 2013, Mass spectrometry reviews.

[32]  Jing-fu Liu,et al.  Time-resolved fluoroimmunoassay as an advantageous analytical method for assessing the total concentration and environmental risk of fluoroquinolones in surface waters. , 2013, Environmental science & technology.

[33]  Tian-Cai Liu,et al.  Development of an improved time-resolved fluoroimmunoassay for simultaneous quantification of C-peptide and insulin in human serum. , 2014, Clinical biochemistry.

[34]  Xu Yan,et al.  Developments in pesticide analysis by multi-analyte immunoassays: a review , 2014 .

[35]  Peiwu Li,et al.  Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts. , 2014, Food chemistry.

[36]  Qi Zhang,et al.  Biotoxin sensing in food and environment via microchip , 2014, Electrophoresis.

[37]  Aaas News,et al.  Book Reviews , 1893, Buffalo Medical and Surgical Journal.