Responses to selection for cadmium resistance in the least killifish, Heterandria formosa

An artificial selection experiment was conducted for six generations to investigate the responses to selection for cadmium resistance in the least killifish, Heterandria formosa. There was a rapid response to selection. After only one generation of selection, two of the three selection lines had an increased resistance to cadmium, while all three selection lines had an elevated resistance by the next generation. After six generations of selection, fish from the selection lines survived about three times as long as control line fish when exposed to cadmium. Realized heritability for cadmium resistance was estimated to be 0.50. Cross-resistance to copper was studied in the F2, F3, and F6 generations; fish from the cadmium-resistant lines had a longer survival time than those from the control lines. Resistance to heat was investigated in the F2, F3, and F5 generations; the cadmium-resistant lines had a significantly shorter survival time than the control lines. Our results showed that evolution of resistance to cadmium was rapid, that cross-resistance to copper occurred, and that the evolution of resistance to cadmium was accompanied by a trade-off (fitness costs) as evidenced by the reduced heat tolerance.

[1]  S. Silver.  Bacterial resistances to toxic metal ions--a review. , 1996, Gene.

[2]  J. Levinton,et al.  Rapid Evolution of Metal Resistance in a Benthic Oligochaete Inhabiting a Metal-polluted Site , 1989 .

[3]  G. Roesijadi,et al.  Resistance to cadmium and parasite infection are inversely related in two strains of a freshwater gastropod , 2002, Environmental toxicology and chemistry.

[4]  M Raymond,et al.  Amplification of an esterase gene is responsible for insecticide resistance in a California Culex mosquito , 1986, Science.

[5]  J. Wise,et al.  Metallothionein gene duplications and metal tolerance in natural populations of Drosophila melanogaster. , 1987, Genetics.

[6]  T. Hutchinson,et al.  MULTIPLE METAL TOLERANCES IN THE GRASS DESCHAMPSIA CESPITOSA (L.) BEAUV. FROM THE SUDBURY SMELTING AREA , 1980 .

[7]  J. Valentine,et al.  Delivering Copper Inside Yeast and Human Cells , 1997, Science.

[8]  M. Rose,et al.  COMPLEX TRADE‐OFFS AND THE EVOLUTION OF STARVATION RESISTANCE IN DROSOPHILA MELANOGASTER , 1996, Evolution; international journal of organic evolution.

[9]  Jie Liu,et al.  Metallothionein: an intracellular protein to protect against cadmium toxicity. , 1999, Annual review of pharmacology and toxicology.

[10]  D. Pascoe,et al.  Heavy metal toxicity to fish and the influence of water hardness , 1986, Archives of environmental contamination and toxicology.

[11]  J. Johnston,et al.  Metabolic resistance to insecticides: heterozygosity at the chromosome II locus in house flies, Musca domestica (Diptera: Muscidae). , 1985, Journal of economic entomology.

[12]  Paul L. Klerks,et al.  Adaptation, Ecological Impacts, and Risk Assessment: Insights from Research at Foundry Cove, Bayou Trepagnier, and Pass Fourchon , 2002 .

[13]  W. Muir.  Estimation of response to selection and utilization of control populations for additional information and accuracy. , 1986, Biometrics.

[14]  Landis Hare,et al.  Experimental evidence for cadmium uptake via calcium channels in the aquatic insect Chironomus staegeri , 1999 .

[15]  M. Timmermans,et al.  Genetic Variation in Toxicant-Stressed Populations: An Evaluation of the “Genetic Erosion” Hypothesis , 2002 .

[16]  F. Rahel.  Selection for Zinc Tolerance in Fish: Results from Laboratory and Wild Populations , 1981 .

[17]  D. Roff,et al.  Quantitative genetics and fitness: lessons from Drosophila , 1987, Heredity.

[18]  Jeff Leips,et al.  The comparative expression of life-history traits and its relationship to the numerical dynamics of four populations of the least killifish , 1999 .

[19]  T. Shono.  Linkage group analysis of carboxylesterase in a malathion resistant strain of the housefly, Musca domestica L. (Diptera: Muscidae) , 1983 .

[20]  A. Schäffer,et al.  Biochemistry of metallothionein. , 1988, Biochemistry.

[21]  L. Posthuma,et al.  Heavy-metal adaptation in terrestrial invertebrates: a review of occurrence, genetics, physiology and ecological consequences , 1993 .

[22]  J. Weis,et al.  Methylmercury tolerance of killifish (Fundulus heteroclitus) embryos from a polluted vs non-polluted environment , 1981 .

[23]  A. Bradshaw,et al.  Heavy Metal Tolerance in Plants , 1971 .

[24]  P. Klerks,et al.  Heritability of resistance to individual contaminants and to contaminant mixtures in the sheepshead minnow (Cyprinodon variegatus) , 2001, Environmental toxicology and chemistry.

[25]  J. Weis,et al.  Genetic adaptation to heavy metals in aquatic organisms: a review. , 1987, Environmental pollution.

[26]  G. Roesijadi.  Metallothioneins in metal regulation and toxicity in aquatic animals , 1992 .

[27]  S. Stearns.  Trade-offs in life-history evolution , 1989 .

[28]  J. Ashida.  Adaptation of Fungi to Metal Toxicants , 1965 .

[29]  G. Georghiou.  The Evolution of Resistance to Pesticides , 1972 .

[30]  L. Nunney.  THE RESPONSE TO SELECTION FOR FAST LARVAL DEVELOPMENT IN DROSOPHILA MELANOGASTER AND ITS EFFECT ON ADULT WEIGHT: AN EXAMPLE OF A FITNESS TRADE‐OFF , 1996, Evolution; international journal of organic evolution.

[31]  R. Sibly,et al.  GENETIC BASIS OF A BETWEEN‐ENVIRONMENT TRADE‐OFF INVOLVING RESISTANCE TO CADMIUM IN DROSOPHILA MELANOGASTER , 1999, Evolution; international journal of organic evolution.

[32]  J. Wilson.  THE COST OF HEAVY‐METAL TOLERANCE: AN EXAMPLE , 1988, Evolution; international journal of organic evolution.