Integrated electrochemical detection for lab on a chip analytical microsystems

The purpose of this study is to develop a portable capillary electrophoresis (CE) "lab-on-a-chip" device that is integrated with on-chip separation and electrochemical detection (ECD) electrodes. Platinum electrodes were photolithographically patterned on soda lime glass substrates and 20 /spl mu/m by 80 /spl mu/m channels were constructed within a second soda lime glass substrate. To fully realize the portability offered by this microfabricated device, a handheld battery powered CE/ECD circuit was also developed. Experimental flow visualization and computer simulation studies were performed to investigate electrokinetic flow within the microfabricated capillaries. CE/ECD "lab-on-a-chip" devices were successfully fabricated. These devices produced identical electrochemical responses for dopamine and catechol with both the portable electronics and conventional bench top power supply and detection equipment. Finally, the computer simulation results were within 10% of the experimental flow visualization results for injection and separation of dichlorofluoroscein.

[1]  S. Lunte,et al.  On-line coupling of in vivo microdialysis with capillary electrophoresis/electrochemistry , 1999 .

[2]  R. Buck,et al.  Microfabricated potentiometric electrodes and their in vivo applications. , 2000, Analytical chemistry.

[3]  D. J. Harrison,et al.  Microfabrication of a Planar Absorbance and Fluorescence Cell for Integrated Capillary Electrophoresis Devices , 1996 .

[4]  J. Alarie,et al.  Effects of the electric field distribution on microchip valving performance , 2000, Electrophoresis.

[5]  Charles S. Henry,et al.  Ceramic microchips for capillary electrophoresis–electrochemistry , 1999 .

[6]  James L. Winkler,et al.  Accessing Genetic Information with High-Density DNA Arrays , 1996, Science.

[7]  S. Jacobson,et al.  Counting single chromophore molecules for ultrasensitive analysis and separations on microchip devices. , 1998, Analytical chemistry.

[8]  J. Michael Ramsey,et al.  Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices , 1994 .

[9]  R A Mathies,et al.  Capillary electrophoresis chips with integrated electrochemical detection. , 1998, Analytical chemistry.

[10]  P. D. Voegel,et al.  Integrated Capillary Electrophoresis/Electrochemical Detection with Metal Film Electrodes Directly Deposited onto the Capillary Tip. , 1997, Analytical chemistry.

[11]  A. Woolley,et al.  Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[12]  A. Ewing,et al.  Electrochemical detection in microcolumn separations. , 1994, Analytical chemistry.

[13]  R N Zare,et al.  Probing individual molecules with confocal fluorescence microscopy. , 1994, Science.

[14]  D. J. Harrison,et al.  Planar chips technology for miniaturization and integration of separation techniques into monitoring systems. Capillary electrophoresis on a chip , 1992 .

[15]  J. Michael Ramsey,et al.  Electrokinetic Focusing in Microfabricated Channel Structures , 1997 .

[16]  R. Mathies,et al.  Single molecule fluorescence burst detection of DNA fragments separated by capillary electrophoresis. , 1995, Analytical chemistry.