A highly sensitive and selective fluorescence off-on probe for the detection of intracellular endogenous tyrosinase activity.

A resorufin-based highly sensitive and selective fluorescence off-on probe with a new recognition moiety for tyrosinase is developed, and applied to detect and image endogenous tyrosinase activity in different living cells.

[1]  Huimin Ma,et al.  Near-Infrared Fluorescent Probe with New Recognition Moiety for Specific Detection of Tyrosinase Activity: Design, Synthesis, and Application in Living Cells and Zebrafish. , 2016, Angewandte Chemie.

[2]  Huimin Ma,et al.  Design principles of spectroscopic probes for biological applications , 2016, Chemical science.

[3]  Xu Yan,et al.  Near-infrared fluorescence nanoprobe for enzyme-substrate system sensing and in vitro imaging. , 2016, Biosensors & bioelectronics.

[4]  Xiaohua Li,et al.  Detection of Misdistribution of Tyrosinase from Melanosomes to Lysosomes and Its Upregulation under Psoralen/Ultraviolet A with a Melanosome-Targeting Tyrosinase Fluorescent Probe. , 2016, Analytical chemistry.

[5]  Zhiqian Guo,et al.  Transforming the recognition site of 4-hydroxyaniline into 4-methoxyaniline grafted onto a BODIPY core switches the selective detection of peroxynitrite to hypochlorous acid. , 2016, Chemical communications.

[6]  Xiaohua Li,et al.  Sensitive and Selective Ratiometric Fluorescence Probes for Detection of Intracellular Endogenous Monoamine Oxidase A. , 2016, Analytical chemistry.

[7]  Wei Chen,et al.  The Development of Fluorescent Probes for Visualizing Intracellular Hydrogen Polysulfides. , 2015, Angewandte Chemie.

[8]  Hui Feng,et al.  Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation. , 2015, ACS applied materials & interfaces.

[9]  Weishi Zheng,et al.  Visual and fluorescent detection of tyrosinase activity by using a dual-emission ratiometric fluorescence probe. , 2015, Analytical chemistry.

[10]  E. Wang,et al.  Ratiometric fluorescence detection of tyrosinase activity and dopamine using thiolate-protected gold nanoclusters. , 2015, Analytical chemistry.

[11]  Yingying Huo,et al.  A mitochondria-targetable fluorescent probe for peroxynitrite: fast response and high selectivity. , 2015, Chemical communications.

[12]  Xiaoming Yang,et al.  Novel synthesis of gold nanoclusters templated with L-tyrosine for selective analyzing tyrosinase. , 2014, Analytica chimica acta.

[13]  Youngmi Kim,et al.  A water-soluble sulfonate-BODIPY based fluorescent probe for selective detection of HOCl/OCl⁻ in aqueous media. , 2014, The Analyst.

[14]  Huimin Ma,et al.  Design strategies for water-soluble small molecular chromogenic and fluorogenic probes. , 2014, Chemical reviews.

[15]  Xiaohua Li,et al.  Sensitive detection of ozone by a practical resorufin-based spectroscopic probe with extremely low background signal , 2013, Scientific Reports.

[16]  Shengyong Yan,et al.  A turn-on fluorescent probe for detection of tyrosinase activity. , 2013, The Analyst.

[17]  Shengyong Yan,et al.  A two-photon fluorescent probe for intracellular detection of tyrosinase activity. , 2012, Chemistry, an Asian journal.

[18]  Juyoung Yoon,et al.  Visual detection of dopamine and monitoring tyrosinase activity using a pyrocatechol violet-Sn4+ complex. , 2011, Chemical communications.

[19]  Jihye Park,et al.  Visualization of tyrosinase activity in melanoma cells by a BODIPY-based fluorescent probe. , 2011, Chemical communications.

[20]  Heinz Decker,et al.  Copper-O2 reactivity of tyrosinase models towards external monophenolic substrates: molecular mechanism and comparison with the enzyme. , 2011, Chemical Society reviews.

[21]  Matthias I. J. Stich,et al.  Multiple fluorescent chemical sensing and imaging. , 2010, Chemical Society reviews.

[22]  O. Wolfbeis,et al.  A near-infrared fluorescent probe for monitoring tyrosinase activity. , 2010, Chemical communications.

[23]  Te-Sheng Chang,et al.  An Updated Review of Tyrosinase Inhibitors , 2009, International journal of molecular sciences.

[24]  E. Bergantino,et al.  The reaction of alpha-synuclein with tyrosinase: possible implications for Parkinson disease. , 2008, The Journal of biological chemistry.

[25]  Itamar Willner,et al.  Analysis of dopamine and tyrosinase activity on ion-sensitive field-effect transistor (ISFET) devices. , 2007, Chemistry.

[26]  R. Varón,et al.  Tyrosinase action on monophenols: evidence for direct enzymatic release of o-diphenol. , 2001, Biochimica et biophysica acta.

[27]  E. Solomon,et al.  Multicopper Oxidases and Oxygenases. , 1996, Chemical reviews.

[28]  R. Varón,et al.  A continuous spectrophotometric method for determining the monophenolase and diphenolase activities of apple polyphenol oxidase. , 1995, Analytical biochemistry.