(2013). Estrogen Receptor Mediates Proliferation of Osteoblastic Cells Stimulated by Estrogen and Mechanical Strain, but Their Acute Down-regulation of the Wnt Antagonist Sost is Mediated by Estrogen Receptor (13),

Background: Strain and estrogens down-regulate Sost /sclerostin and stimulate osteoblastic proliferation. Results: ER (cid:1) inhibition prevents proliferation. ER (cid:2) inhibition prevents Sost down-regulation by strain or estradiol. Sclerostin prevents proliferation following strain and not estradiol. Conclusion: ER (cid:1) promotes proliferation, and ER (cid:2) mediates Sost down-regulation following estradiol ligand stimulation and ligand independently following strain. Significance: Selective ER modulators could promote osteogenesis through differential regulation of Sost and proliferation. Mechanical strain and estrogens both stimulate osteoblast proliferation through estrogen receptor (ER)-mediated effects, and both down-regulate the Wnt antagonist Sost/ sclerostin. Here, we investigate the differential effects of ER (cid:1) and - (cid:2) in these processes in mouse long bone-derived osteoblastic cells and human Saos-2 cells.Recruitmenttothecellcyclefollowingstrainor17 (cid:2) -estradiol

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