Modification of the Cytosensor™ microphysiometer to simultaneously measure extracellular acidification and oxygen consumption rates

Abstract The Cytosensor™ microphysiometer has been modified by incorporating several platinum electrodes into the plunger head. The electrodes have been used to measure simultaneously the change in extracellular oxygen consumption rates and extracellular acidification rates during addition of various non-physiologic agents: 2-deoxy- d -glucose, sodium fluoride, paraoxon-ethyl, and antimycin A. Measurement of two parameters, oxygen and acidification, enables investigation of the aerobic and anaerobic metabolic consequences of these agents. Nafion membranes cast onto the platinum electrodes were useful in minimizing adsorption of cell-related interferents.

[1]  P. O'Brien,et al.  Prevention of cyanide-induced cytotoxicity by nutrients in isolated rat hepatocytes. , 1994, Toxicology and applied pharmacology.

[2]  G. Sittampalam,et al.  Amperometric determination of glucose at parts per million levels with immobilized glucose oxidase: An undergraduate experiment , 1982 .

[3]  Donald Voet,et al.  Fundamentals of Biochemistry , 1999 .

[4]  F. Hafner,et al.  Cytosensor Microphysiometer: technology and recent applications. , 2000, Biosensors & bioelectronics.

[5]  J. Leddy,et al.  Density and solubility of nafion:  recast, annealed, and commercial films. , 1996, Analytical chemistry.

[6]  J. Valdés,et al.  Cytotoxicity of organophosphate anticholinesterases , 1999, In Vitro Cellular & Developmental Biology - Animal.

[7]  T. Ward,et al.  Organophosphorus compounds preferentially affect second messenger systems coupled to M2/M4 receptors in rat frontal cortex , 1996, Brain Research Bulletin.

[8]  J. Buccafusco,et al.  Inhibition of nerve growth factor signaling by peroxynitrite , 2001 .

[9]  J. W. Parce,et al.  The light-addressable potentiometric sensor: principles and biological applications. , 1994, Annual review of biophysics and biomolecular structure.

[10]  C. Eintrei,et al.  Effects of propofol on extracellular acidification rates in primary cortical cell cultures: application of silicon microphysiometry to anaesthesia. , 1999, British journal of anaesthesia.

[11]  B. Jortner,et al.  Organophosphorus compound-induced apoptosis in SH-SY5Y human neuroblastoma cells. , 2000, Toxicology and applied pharmacology.

[12]  C. Beeson,et al.  Continuous measurement of glucose utilization in heart myoblasts. , 2002, Analytical biochemistry.

[13]  G. S. Wilson,et al.  Biosensors : fundamentals and applications , 1987 .

[14]  W. Cascio,et al.  Electrodeposited iridium oxide pH electrode for measurement of extracellular myocardial acidosis during acute ischemia. , 1998, Analytical chemistry.

[15]  J. Baur,et al.  Electrochemical deposition of iridium (IV) oxide from alkaline solutions of iridium(III) oxide , 1998 .

[16]  J. W. Parce,et al.  Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Jeng,et al.  Cytotoxicity of sodium fluoride on human oral mucosal fibroblasts and its mechanisms , 1998, Cell Biology and Toxicology.

[18]  C. Tack,et al.  Vascular Effects of Metabolic Inhibition by 2-Deoxy-D-Glucose in Humans , 2001, Journal of cardiovascular pharmacology.

[19]  L. Formigli,et al.  Aponecrosis: Morphological and biochemical exploration of a syncretic process of cell death sharing apoptosis and necrosis , 2000, Journal of cellular physiology.

[20]  J. W. Parce,et al.  Detection of cell-affecting agents with a silicon biosensor. , 1989, Science.