Suppressing role of miR-520a-3p in breast cancer through CCND1 and CD44.

Recent studies show that many microRNAs (miRNAs) are found to play important roles in breast cancer, however, most of miRNAs are not investigated completely. In the present study, significant down-regulation of miR-520a-3p was found in the breast cancer tissues and cell lines. The restoration of miR-520a-3p expression in breast cancer cells could inhibit cell proliferation, migration and invasion. In addition, miR-520a-3p was able to induce breast cancer cell apoptosis. Luciferase assay was used to confirm that CCND1 and CD44 were the direct target genes of miR-520a-3p. The ectopic expression of miR-520a-3p repressed CCND1 and CD44 expression on post-transcriptional levels in breast cancer cells. This study suggests that miR-520a-3p may act as an optional method for breast cancer therapy.

[1]  Yunfeng Fu,et al.  Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development , 2016, Oncotarget.

[2]  A. Magklara,et al.  Revealing the Complexity of Breast Cancer by Next Generation Sequencing , 2015, Cancers.

[3]  Hui Ling,et al.  MicroRNA Processing and Human Cancer , 2015, Journal of clinical medicine.

[4]  Isabella Castiglioni,et al.  MicroRNAs: New Biomarkers for Diagnosis, Prognosis, Therapy Prediction and Therapeutic Tools for Breast Cancer , 2015, Theranostics.

[5]  G. Calin,et al.  MicroRNAs in cancer: from developmental genes in worms to their clinical application in patients , 2015, British Journal of Cancer.

[6]  Takahiro Ochiya,et al.  The Roles of MicroRNAs in Breast Cancer , 2015, Cancers.

[7]  Hongchuan Jin,et al.  MicroRNAs as Potential Biomarkers in Cancer: Opportunities and Challenges , 2015, BioMed research international.

[8]  Baoan Ma,et al.  MicroRNA profiling identifies MiR-195 suppresses osteosarcoma cell metastasis by targeting CCND1 , 2015, Oncotarget.

[9]  G. Lou,et al.  miR-211 suppresses epithelial ovarian cancer proliferation and cell-cycle progression by targeting Cyclin D1 and CDK6 , 2015, Molecular Cancer.

[10]  Q. Tan,et al.  The microRNA-520a-3p inhibits proliferation, apoptosis and metastasis by targeting MAP3K2 in non-small cell lung cancer. , 2015, American journal of cancer research.

[11]  T. Zeng,et al.  MicroRNA-9 promotes tumor metastasis via repressing E-cadherin in esophageal squamous cell carcinoma , 2014, Oncotarget.

[12]  Quan-yong He,et al.  MiR-199a inhibits the ability of proliferation and migration by regulating CD44-Ezrin signaling in cutaneous squamous cell carcinoma cells. , 2014, International journal of clinical and experimental pathology.

[13]  Momoko Yoshikawa,et al.  Macrophage-derived reactive oxygen species suppress miR-328 targeting CD44 in cancer cells and promote redox adaptation. , 2014, Carcinogenesis.

[14]  R. Dahiya,et al.  miRNA-708 control of CD44(+) prostate cancer-initiating cells. , 2012, Cancer research.

[15]  X. Wan,et al.  MicroRNA‐199a targets CD44 to suppress the tumorigenicity and multidrug resistance of ovarian cancer‐initiating cells , 2012, The FEBS journal.

[16]  Gabriel Wong,et al.  Stem Cell Marker (Nanog) and Stat-3 Signaling Promote MicroRNA-21 Expression and Chemoresistance in Hyaluronan/CD44-activated Head and Neck Squamous Cell Carcinoma Cells , 2011, Oncogene.

[17]  K. Kelnar,et al.  The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44. , 2011, Nature medicine.

[18]  Thomas D. Schmittgen,et al.  miR-199a-3p targets CD44 and reduces proliferation of CD44 positive hepatocellular carcinoma cell lines. , 2010, Biochemical and biophysical research communications.

[19]  Yaou Zhang,et al.  MicroRNA miR-328 Regulates Zonation Morphogenesis by Targeting CD44 Expression , 2008, PloS one.

[20]  Lin Zhang,et al.  The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis , 2008, Nature Cell Biology.

[21]  Y. Lee,et al.  miR-106b modulates cancer stem cell characteristics through TGF-β/Smad signaling in CD44-positive gastric cancer cells , 2014, Laboratory Investigation.

[22]  K. Iczkowski,et al.  MicroRNAs 373 and 520c are downregulated in prostate cancer, suppress CD44 translation and enhance invasion of prostate cancer cells in vitro. , 2009, International journal of clinical and experimental pathology.