2022-RA-951-ESGO Table 1 Comparison of perioperative and postoperative morbidity and mortality between both groups Variables Group 1 Upper+Lower (n=55) Group 2 Lower (n=93) P value Intraoperative Complications (n%) Vascular 12 (21.8) 21 (22.6) 0.914 Gastrointestinal 6 (10.9) 9 (9.7) 0.810 Urinary 8 (14.5) 6 (6.5) 0.104 Diaphragm injury 7 (12.7) 0 (0) 0.001 Blood transfusion 16 (29.1) 12 (13.0) 0.017 Postoperative Complications (n%) Length of stay; median; [minmax]; d 8 [1–45] 6 [1–68] 0.002 Atelectasis, pneumonia, effusion 11 (20) 8 (8.6) 0.045 Bleeding 2 (3.6) 2 (2.2) 0.628 Abscess 5 (9.1) 2 (2.2) 0.102 Ileus 2 (3.6) 4 (4.3) 1.000 Wound infection 19 (34.5) 14 (15.1) 0.006 Anostomotic leak 5 (9.1) 2 (2.2) 0.102 Hernia 4 (7.3) 7 (7.5) 0.955 Deep vein thrombosis 4 (7.3) 1 (1.1) 0.064 Need for intensive care unit 32 (58.2) 32 (34.4) 0.005 Mortality at 30 days 1 (1.8) 1 (1.1) 1.000 Conclusion Upper abdominal surgery is a valid procedure for optimal cytoreduction in ovarian cancer. However, there is a greater risk of some complications occurring compared to lower abdominal surgery. Upper abdominal surgery in advanced ovarian cancer can be applied to patients with an acceptable complication profile when the possible survival advantage to be obtained is taken into account. 2022-RA-953-ESGO SERINE METABOLISM REMODELING AFTER PLATINUM-BASED CHEMOTHERAPY IS A NEW VULNERABILITY IN RESISTANT OVARIAN CANCER PATIENTS Tom van Nyen, Mélanie Planque, Lilian van Wagensveld, Esther Zaal, Lara Rizzotto, Wout de Wispelaere, Ali Talebi, Pierre-René Körner, Gabe Sonke, Hugo Horlings, Ben Davidson, Johan Swinnen, Celia Berkers, Reuven Agami, Sarah-Maria Fendt, Daniela Annibali, Frédéric Amant. Oncology, KU Leuven, Leuven, Belgium; VIB-KU Leuven, Leuven, Belgium; The Netherlands Cancer Institute, Amsterdam, Netherlands; Utrecht University, Utrecht, Netherlands; KU Leuven, Leuven, Belgium; Medical Oncology, The Netherlands Cancer Institute, Amsterdam, Netherlands; Oslo University Hospital, Oslo, Norway; UZ Leuven/KU Leuven, Leuven, Belgium 10.1136/ijgc-2022-ESGO.605 Introduction/Background Resistance to platinum-based chemotherapy represents a major challenge for the management of many cancer patients, particularly those with epithelial ovarian cancer. Despite improved knowledge of the molecular determinants of platinum resistance, lack of clinical applicability still limits exploitation of many potential targets, leaving patients with limited options. Rewiring of cellular metabolism is now recognized as one of the hallmarks of cancer cells, and the ability of cancer cells to plastically change their use of nutrients and amino acids has been linked to cell adaptation to death signaling and survival. Serine biosynthesis has been linked to cancer growth and poor prognosis in various cancer types, however its role in platinum-resistant ovarian cancer has not been investigated before. Methodology Phosphoglycerate dehydrogenase (PHGDH) expression has been determined in matched biopsies from ovarian cancer patients collected longitudinally at diagnosis and at relapse after platinum treatment. Metabolomic and molecular analyses have been performed on the isogenic ovarian cancer cell lines A2780 (wt/cis). Results have been confirmed in sensitive, resistant and matched ovarian cancer patient-derived xenograft (PDX) models and PDX-derived organoid cultures. Results We discovered that a subgroup of resistant tumors decreases phosphoglycerate dehydrogenase (PHGDH) expression at relapse after platinum-based chemotherapy. Mechanistically, we observe that this phenomenon is accompanied by a specific oxidized nicotinamide adenine dinucleotide (NAD) regenerating phenotype, which helps tumor cells in sustaining Poly (ADP-ribose) polymerase (PARP) activity under platinum treatment. Consequently, combining carboplatin and PARP or NAD-synthesis inhibitors is effective in resistant models showing decreased serine synthesis activity. Conclusion Our findings, accepted for publication in Nature Communications, have immediate and strong clinical applicability, because they identify alterations in serine and NAD+ metabolism as actionable vulnerabilities in a subgroup of platinum resistant ovarian cancers, and provide a rationale to test novel combinatorial therapeutic approaches to target resistance in these patients. Abstracts Int J Gynecol Cancer 2022;32(Suppl 2):A1–A504 A285 on D ecem er 6, 2022 by gest. P rocted by coright. http/ijgc.bm jcom / nt J G ynecol C acer: frst pulished as 10.11ijgc-2022-E S G O .05 on 20 O cber 222. D ow nladed fom