Genetic Modification of Herbaceous Plants for Feed and Fuel

Referee: Dr. E. Charles Brummer, Forage Breeding and Genetics, 1204 Agromonomy, Iowa State University, Ames, IA 50011 Much of the research on the genetic modification of herbaceous plant cell walls has been conducted to improve the utilization of forages by ruminant livestock. The rumen of these animals is basically an anaerobic fermentation vat in which the micro flora break down the complex polysaccharides of plant cell walls into simpler compounds that can be further digested and absorbed by the mammalian digestive system. Research on improving the forage digestibility of switchgrass, Panicum virgatum L., and other herbaceous species has demonstrated that genetic improvements can be made in forage quality that can have significant economic value. To meet future energy needs, herbaceous biomass will need to be converted into a liquid fuel, probably ethanol, via conversion technologies still under development. If feedstock quality can be genetically improved, the economics and efficiency of the conversio...

[1]  C. J. Nelson,et al.  An introduction to grassland agriculture , 1995 .

[2]  G. A. Jung,et al.  Leaf and Stem Forage Quality of Big Bluestem and Switchgrass1 , 1983 .

[3]  K. Vogel,et al.  Forage Quality and Performance of Yearlings Grazing Switchgrass Strains Selected for Differing Digestibility , 1988 .

[4]  K. Vogel,et al.  Alkali-Labile Cell-Wall Phenolics and Forage Quality in Switchgrasses selected for Differing Digestibility , 1990 .

[5]  A. Komamine,et al.  Changes in enzyme activities involved in formation and interconversion of UDP-sugars during the cell cycle in a synchronous culture of Catharanthus roseus , 1985 .

[6]  R. Helm,et al.  Cell Wall Cross-Linking in Grasses by Ferulates and Diferulates , 1998 .

[7]  J. C. Burns,et al.  Effectiveness of Index Selection for Switchgrass Forage Yield and Quality , 1988 .

[8]  F. Samson,et al.  Prairie conservation in North America , 1994 .

[9]  J. Ralph,et al.  Accuracy of Klason lignin and acid detergent lignin methods as assessed by bomb calorimetry. , 1999, Journal of agricultural and food chemistry.

[10]  K. Vogel,et al.  Registration of 'Trailblazer' Switchgrass , 1991 .

[11]  S. Knapp Marker-Assisted Selection as a Strategy for Increasing the Probability of Selecting Superior Genotypes , 1998 .

[12]  A. Hopkins Genetic variation among switchgrasses for agronomic, forage quality, and biofuel traits , 1993 .

[13]  Robert B. Mitchell,et al.  Predicting Forage Quality in Switchgrass and Big Bluestem , 2001 .

[14]  K. Vogel,et al.  Grazing selectivity and in vivo digestibility of switchgrass strains selected for differing digestibility. , 1989, Journal of Animal Science.

[15]  G. C. Marten,et al.  Near infrared reflectance spectroscopy (NIRS): analysis of forage quality , 1989 .

[16]  D. Buxton,et al.  Forage Cell Wall Structure and Digestibility , 1993 .

[17]  K. Vogel,et al.  Accomplishments and Impact from Breeding for Increased Forage Nutritional Value , 1999 .

[18]  C. Sheaffer,et al.  Forage Quality Variation in the U.S. Alfalfa Core Collection , 1997 .

[19]  Christopher P. Bonin,et al.  The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Chung-Jui Tsai,et al.  Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees , 1999, Nature Biotechnology.

[21]  J. Wilson,et al.  Effect of temperature on anatomical structure, rate of digestion of different cell types, and dry matter digestibility of leaf and stem of some tropical and temperature forage grasses. , 1991 .

[22]  H. D. Hughes,et al.  Forages : The Science Of Grassland Agriculture , 1952 .

[23]  Hansang Jung,et al.  Selection and evaluation of smooth bromegrass clones with divergent lignin or etherified ferulic acid concentration , 1999 .

[24]  K. Vogel,et al.  Lignification of switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii) plant parts during maturation and its effect on fibre degradability , 1992 .

[25]  J. Ralph,et al.  p-Hydroxyphenyl, Guaiacyl, and Syringyl Lignins Have Similar Inhibitory Effects on Wall Degradability , 1997 .

[26]  K. Moore,et al.  Registration of ‘Shawnee‘ Switchgrass , 1996 .

[27]  C. Paul,et al.  QTL mapping in testcrosses of European flint lines of maize. II. Comparison of different testers for forage quality traits , 1997 .

[28]  D. Mertens,et al.  Correlation of acid detergent lignin and Klason lignin with digestibility of forage dry matter and neutral detergent fiber. , 1997, Journal of dairy science.

[29]  T. Fukui,et al.  UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. , 1990, Journal of biochemistry.

[30]  I. Morrison Hemicellulosic contamination of acid detergent residues and their replacement by cellulose residues in cell wall analysis , 1980 .

[31]  M. Polacco,et al.  Synergy of Empirical Breeding, Marker‐Assisted Selection, and Genomics to Increase Crop Yield Potential , 1999 .

[32]  G. C. Marten,et al.  BIDIRECTIONAL SELECTION FOR NEUTRAL DETERGENT FIBER AND YIELD IN REED CANARYGRASS , 1988 .

[33]  J. Coors,et al.  Relationship between Plant Composition and European Corn Borer Resistance in Three Maize Populations , 1997 .

[34]  F. Vignols,et al.  The brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferase. , 1995, The Plant cell.

[35]  P. Beyer,et al.  Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. , 2000, Science.

[36]  Jerome J. Workman,et al.  Application of NIR spectroscopy to agricultural products. In 'Handbook of Near-infrared Analysis'.(E , 1992 .

[37]  Jing Zhang,et al.  Engineering the Provitamin A (b-Carotene) Biosynthetic Pathway into (Carotenoid-Free) , 2000 .

[38]  K. Alexandrova,et al.  Micropropagation of switchgrass by node culture. , 1996, Crop science.

[39]  D. R. Buxton,et al.  Water‐Stress Effects on Alfalfa Forage Quality After Adjustment for Maturity Differences , 1989 .

[40]  A. Kilian,et al.  Cloning and characterization of several cDNAs for UDP-glucose pyrophosphorylase from barley (Hordeum vulgare) tissues. , 1996, Gene.

[41]  K. Moore,et al.  Genotypic variability and genotype × environment interactions among switchgrass accessions from the midwestern USA , 1995 .

[42]  K. Vogel,et al.  Chloroplast DNA and nuclear DNA content variations among cultivars of switchgrass , 1996 .

[43]  A. Cornu,et al.  Cell Wall Degradability of Transgenic Tobacco Stems in Relation to Their Chemical Extraction and Lignin Quality , 1996 .

[44]  R. J. Baker Selection Indices in Plant Breeding , 1986 .

[45]  T. Umezawa,et al.  5-Hydroxyconiferyl Aldehyde Modulates Enzymatic Methylation for Syringyl Monolignol Formation, a New View of Monolignol Biosynthesis in Angiosperms* , 2000, The Journal of Biological Chemistry.

[46]  C. Mcmillan,et al.  ECOTYPIC DIFFERENTIATION WITHIN FOUR NORTH AMERICAN PRAIRIE GRASSES. II. BEHAVIORAL VARIATION WITHIN TRANSPLANTED COMMUNITY FRACTIONS , 1964 .

[47]  Jacqueline Grima-Pettenati,et al.  Down-regulation of Cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants , 2002 .

[48]  S. Gupta,et al.  Somatic embryogenesis and plant regeneration from suspension cultures of switchgrass , 1999 .

[49]  C. Chapple,et al.  An Arabidopsis mutant defective in the general phenylpropanoid pathway. , 1992, The Plant cell.

[50]  Gerald A. Tuskan,et al.  Diversity among Populations of Switchgrass Based on RAPD Markers , 1996 .

[51]  J. Burns,et al.  Prediction of Cell Wall Carbohydrates and Quality in Panicum Species by Near Infrared Reflectance Spectroscopy , 1988 .

[52]  K. Moore,et al.  Predicted and Realized Gains from Selection for In Vitro Dry Matter Digestibility and Forage Yield in Switchgrass , 1993 .

[53]  W Herth,et al.  Molecular analysis of cellulose biosynthesis in Arabidopsis. , 1998, Science.

[54]  Marc Van Montagu,et al.  Biosynthesis and genetic engineering of lignin , 1998 .

[55]  C. Chapple,et al.  Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition. , 1997, The Plant cell.

[56]  K. Moore,et al.  Describing and Quantifying Growth Stages of Perennial Forage Grasses , 1991 .

[57]  R. Dixon,et al.  Reduced Lignin Content and Altered Lignin Composition in Transgenic Tobacco Down-Regulated in Expression of L-Phenylalanine Ammonia-Lyase or Cinnamate 4-Hydroxylase , 1997, Plant physiology.

[58]  N. Lewis,et al.  Lignin and Lignan Biosynthesis , 1998 .

[59]  J. Wilson Organization of Forage Plant Tissues , 1993 .

[60]  Ronald D. Hatfield,et al.  Composition of cell walls isolated from cell types of grain sorghum stems , 1999 .

[61]  K. Moore,et al.  Genotype Effects and Genotype by Environment Interactions for Traits of Elite Switchgrass Populations , 1995 .

[62]  B. Conger,et al.  Plant Regeneraton from Callus Cultures of Switchgrass , 1994 .

[63]  W. Fehr,et al.  Hybridization of crop plants , 1980 .

[64]  R. Sederoff,et al.  Abnormal lignin in a loblolly pine mutant. , 1997, Science.

[65]  R. Sederoff,et al.  Inheritance, gene expression, and lignin characterization in a mutant pine deficient in cinnamyl alcohol dehydrogenase. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[66]  Cathie Martin,et al.  The AmMYB308 and AmMYB330 Transcription Factors from Antirrhinum Regulate Phenylpropanoid and Lignin Biosynthesis in Transgenic Tobacco , 1998, Plant Cell.

[67]  D. Shibata,et al.  Increase of Cinnamaldehyde Groups in Lignin of Transgenic Tobacco Plants Carrying an Antisense Gene for Cinnamyl Alcohol Dehydrogenase , 1995 .

[68]  Robert B. Mitchell,et al.  Predicting Developmental Morphology in Switchgrass and Big Bluestem , 1997 .

[69]  C. Lapierre,et al.  NMR characterization of altered lignins extracted from tobacco plants down-regulated for lignification enzymes cinnamylalcohol dehydrogenase and cinnamoyl-CoA reductase. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[70]  H. Jung,et al.  Lignification of plant cell walls: impact of genetic manipulation. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[71]  H. Blackbourn Less lignin is more cellulose , 1999 .

[72]  Matt A. Sanderson,et al.  Switchgrass Biomass Composition during Morphological Development in Diverse Environments , 1995 .

[73]  R. Tenhaken,et al.  Cloning of an Enzyme That Synthesizes a Key Nucleotide-Sugar Precursor of Hemicellulose Biosynthesis from Soybean:UDP-Glucose Dehydrogenase , 1996, Plant physiology.

[74]  O. Shoseyov,et al.  Bacterial cellulose-binding domain modulates in vitro elongation of different plant cells , 1998, Plant physiology.

[75]  N. Sakurai,et al.  Culm Brittleness of Barley (Hordeum vulgare L.) Mutants Is Caused by Smaller Number of Cellulose Molecules in Cell Wall. , 1991, Plant physiology.

[76]  F. M. Engels,et al.  Alfalfa stem tissues: cell-wall development and lignification. , 1998 .

[77]  D. Buxton,et al.  Digestibility and cell-wall components of alfalfa following selection for divergent herbage lignin concentration , 1990 .

[78]  P. V. Soest Nutritional Ecology of the Ruminant , 1994 .

[79]  Deborah P. Delmer,et al.  CELLULOSE BIOSYNTHESIS: Exciting Times for A Difficult Field of Study. , 1999, Annual review of plant physiology and plant molecular biology.

[80]  M. Casler Phenotypic Recurrent Selection Methodology for Reducing Fiber Concentration in Smooth Bromegrass , 1999 .

[81]  D. Buxton,et al.  A Comparison of the Insoluble Residues Produced by the Klason Lignin and Acid Detergent Lignin Procedures , 1994 .

[82]  C. Chapple,et al.  Impact of lignin composition on cell‐wall degradability in an Arabidopsis mutant , 1999 .

[83]  Ronald D. Hatfield,et al.  Lignin-ferulate cross-links in grasses: active incorporation of ferulate polysaccharide esters into ryegrass lignins , 1995 .

[84]  K. Edwards,et al.  Brown-midrib maize (bm1)--a mutation affecting the cinnamyl alcohol dehydrogenase gene. , 1998, The Plant journal : for cell and molecular biology.

[85]  A. Hallauer,et al.  Quantitative Genetics in Maize Breeding , 1981 .

[86]  D. Buxton,et al.  Water-Deficit Effects on Cell-Wall Composition and In Vitro Degradability of Structural Polysaccharides from Alfalfa Stems , 1996 .

[87]  D. R. Cornelius,et al.  Differences in Plant Type and Reaction to Rust among Several Collections of Panicum Virgatum L.1 , 1941 .

[88]  S. Knapp Marker‐Assisted Selection as a Strategy for Increasing the Probability of Selecting Superior Genotypes , 1998 .

[89]  K. Moore,et al.  Ammonia-Labile Bonds in High- and Low-Digestibility Strains of Switchgrass , 1991 .

[90]  G. Hill,et al.  Comparison of Tifton 85 and Coastal bermudagrasses for yield, nutrient traits, intake, and digestion by growing beef steers. , 1999, Journal of animal science.

[91]  D. Buxton,et al.  Morphology of Alfalfa Divergently Selected for Herbage Lignin Concentration , 1989 .

[92]  C. Halpin,et al.  Effect of down-regulation of cinnamyl alcohol dehydrogenase on cell wall composition and on degradability of tobacco stems , 1998 .

[93]  R. W. Allard Principles of Plant Breeding , 1960 .

[94]  T. Umezawa,et al.  Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[95]  D. Sleper,et al.  Alteration of Plants via Genetics and Plant Breeding , 1994 .

[96]  S. Cutler,et al.  The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis. , 1999, The Plant cell.

[97]  C. Mcmillan,et al.  The Role of Ecotypic Variation in the Distribution of the Central Grassland of North America , 1959 .

[98]  C. McSweeney,et al.  Acid detergent dispersible lignin in tropical grasses , 1994 .

[99]  N. Raikhel,et al.  Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis. , 1999, Science.

[100]  K. Moore,et al.  Native North American grasses. , 1993 .

[101]  D. Falconer,et al.  Introduction to Quantitative Genetics. , 1962 .

[102]  D. Delmer,et al.  Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[103]  A. Boudet,et al.  Impact of different levels of cinnamyl alcohol dehydrogenase down-regulation on lignins of transgenic tobacco plants , 1997, Planta.

[104]  C. Chapple,et al.  Altered Growth and Cell Walls in a Fucose-Deficient Mutant of Arabidopsis , 1993, Science.

[105]  D. S. Fisher,et al.  Heritability of Cell Wall Carbohydrates in Switchgrass , 1988 .

[106]  D. Falconer Introduction to quantitative genetics. 1. ed. , 1984 .

[107]  G. Fahey,et al.  Forage Quality, Evaluation, and Utilization , 1994 .

[108]  Bernard Fritig,et al.  Altered lignin composition in transgenic tobacco expressing O-methyltransferase sequences in sense and antisense orientation , 1995 .

[109]  C. Somerville,et al.  Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. , 1997, The Plant cell.

[110]  J. Coors,et al.  European corn borer resistance and cell wall composition of three maize populations , 1990 .

[111]  D. Delmer,et al.  9 – Biosynthesis of Plant Cell Walls , 1988 .

[112]  K. Alexandrova,et al.  In Vitro Development of Inflorescences from Switchgrass Nodal Segments , 1996 .

[113]  M. Casler Correlated responses in forage yield and nutritional value from phenotypic recurrent selection for reduced fiber concentration in smooth bromegrass , 1999, Theoretical and Applied Genetics.

[114]  R. Andersson,et al.  Total dietary fiber determined as neutral sugar residues, uronic acid residues, and Klason lignin (the Uppsala method): collaborative study. , 1995, Journal of AOAC International.

[115]  K. Vogel,et al.  Divergent Selection for In Vitro Dry Matter Digestibility in Switchgrass1 , 1981 .

[116]  L. Newell Effects of Strain Source and Management Practice on Forage Yields of Two Warm‐Season Prairie Grasses1 , 1968 .

[117]  D. Buxton,et al.  Environmental and Genetic Effects on Cell Wall Composition and Digestibility , 1993 .

[118]  R. Service Seed-Sterilizing 'Terminator Technology' Sows Discord , 1998, Science.

[119]  M. Casler Structural Responses to Selection for Reduced Fiber Concentration in Smooth Bromegrass , 1999 .

[120]  Ben Hui Liu,et al.  Statistical Genomics: Linkage, Mapping, and QTL Analysis , 1997 .

[121]  N. Sakurai,et al.  Culm strength of barley : correlation among maximum bending stress, cell wall dimensions, and cellulose content. , 1989, Plant physiology.

[122]  D. Duvick How Much Caution in the Fields? , 1999, Science.

[123]  G. W. Fick,et al.  Morphological Stage of Development as a Predictor of Alfalfa Herbage Quality1 , 1983 .

[124]  L. E. Moser,et al.  Switchgrass, Big Bluestem, and Indiangrass , 1995 .

[125]  R. A. Sutherland,et al.  Quantitative Genetics and Selection in Plant Breeding. , 1987 .

[126]  F. Samson,et al.  Prairie conservation: preserving North America's most endangered ecosystem. , 1997 .

[127]  C. Benning,et al.  Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli. , 1996, Archives of biochemistry and biophysics.

[128]  D. Delmer,et al.  A mutant of Arabidopsis thaliana displaying altered patterns of cellulose deposition , 1995 .