Gene regulation during late embryogenesis: the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product.

The Arabidopsis mutants fus3 and abi3 show pleiotropic effects during embryogenesis including reduced levels of transcripts encoding embryo-specific seed proteins. To investigate the interaction between the B3-domain-containing transcription factors FUS3 and ABI3 with the RY cis-motif, conserved in many seed-specific promoters, a promoter analysis as well as band-shift experiments were performed. The analysis of promoter mutants revealed the structural requirements for the function of the RY cis-element. It is shown that both the nucleotide sequence and the alternation of purin and pyrimidin nucleotides (RY character) are essential for the activity of the motif. Further, it was shown that FUS3 and ABI3 can act independently of each other in controlling promoter activity and that the RY cis-motif is a target for both transcription factors. For FUS3, which is so far the smallest known member of the B3-domain family, a physical interaction with the RY motif was established. The functional and biochemical data demonstrate that the regulators FUS3 and ABI3 are essential components of a regulatory network acting in concert through the RY-promoter element to control gene expression during late embryogenesis and seed development.

[1]  U. Wobus,et al.  The FUS3 gene of Arabidopsis thaliana is a regulator of gene expression during late embryogenesis , 1994 .

[2]  C. D. Dickinson,et al.  RY repeats are conserved in the 5'-flanking regions of legume seed- protein genes , 1988, Nucleic Acids Res..

[3]  U. Wobus,et al.  The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. , 1986, Nucleic acids research.

[4]  H. Goodman,et al.  Isolation of the Arabidopsis ABI3 gene by positional cloning. , 1992, The Plant cell.

[5]  D. McCarty,et al.  The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator , 1991, Cell.

[6]  N. Nielsen,et al.  5'CATGCAT-3' Elements Modulate the Expression of Glycinin Genes. , 1992, Plant physiology.

[7]  U. Wobus,et al.  Seed maturation: genetic programmes and control signals. , 1999, Current opinion in plant biology.

[8]  F. Parcy,et al.  The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development. , 1997, The Plant cell.

[9]  D. Inzé,et al.  Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within the legumin box is essential for tissue-specific expression of a legumin gene. , 1992, The Plant journal : for cell and molecular biology.

[10]  C. Kao,et al.  The conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity. , 1997, The Plant cell.

[11]  D. Robertson,et al.  Molecular Analysis of viviparous-1: An Abscisic Acid-Insensitive Mutant of Maize. , 1989, The Plant cell.

[12]  F. Parcy,et al.  Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid. , 1994, The Plant cell.

[13]  Robert B Goldberg,et al.  Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells , 1998, Cell.

[14]  G. Hagen,et al.  ARF1, a transcription factor that binds to auxin response elements. , 1997, Science.

[15]  L. Josefsson,et al.  Structure of a gene encoding the 1.7 S storage protein, napin, from Brassica napus. , 1987, The Journal of biological chemistry.

[16]  D. Meinke.  Molecular Genetics of Plant Embryogenesis , 1995 .

[17]  P. Herrmann,et al.  FUSCA3 encodes a protein with a conserved VP1/ABI3-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana. , 1998, The Plant journal : for cell and molecular biology.

[18]  fusca3: A Heterochronic Mutation Affecting Late Embryo Development in Arabidopsis. , 1994, The Plant cell.

[19]  C. M. Karssen,et al.  In Vivo Inhibition of Seed Development and Reserve Protein Accumulation in Recombinants of Abscisic Acid Biosynthesis and Responsiveness Mutants in Arabidopsis thaliana. , 1989, Plant physiology.

[20]  M. West,et al.  LEAFY COTYLEDON1 Is an Essential Regulator of Late Embryogenesis and Cotyledon Identity in Arabidopsis. , 1994, The Plant cell.

[21]  D. McCarty,et al.  The quiescent/colorless alleles of viviparous1 show that the conserved B3 domain of VP1 is not essential for ABA-regulated gene expression in the seed. , 1997, The Plant journal : for cell and molecular biology.

[22]  T. Wohlfarth,et al.  Ectopic expression of a novel MYB gene modifies the architecture of the Arabidopsis inflorescence. , 1998, The Plant journal : for cell and molecular biology.

[23]  D. Meinke.  A Homoeotic Mutant of Arabidopsis thaliana with Leafy Cotyledons , 1992, Science.

[24]  B. Luisi,et al.  Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding. , 1999, Journal of molecular biology.

[25]  P. Sharp,et al.  Sequence-specific DNA binding of the B-cell-specific coactivator OCA-B. , 1996, Genes & development.

[26]  A. Bobb,et al.  Conserved RY-repeats mediate transactivation of seed-specific promoters by the developmental regulator PvALF. , 1997, Nucleic acids research.

[27]  D. McCarty.  Genetic control and integration of maturation and germination pathways in seed development , 1995 .