Bmc Complementary and Alternative Medicine Ginger Extract Inhibits Lps Induced Macrophage Activation and Function

BackgroundMacrophages play a dual role in host defence. They act as the first line of defence by mounting an inflammatory response to antigen exposure and also act as antigen presenting cells and initiate the adaptive immune response. They are also the primary infiltrating cells at the site of inflammation. Inhibition of macrophage activation is one of the possible approaches towards modulating inflammation. Both conventional and alternative approaches are being studied in this regard. Ginger, an herbal product with broad anti inflammatory actions, is used as an alternative medicine in a number of inflammatory conditions like rheumatic disorders. In the present study we examined the effect of ginger extract on macrophage activation in the presence of LPS stimulation.MethodsMurine peritoneal macrophages were stimulated by LPS in presence or absence of ginger extract and production of proinflammatory cytokines and chemokines were observed. We also studied the effect of ginger extract on the LPS induced expression of MHC II, B7.1, B7.2 and CD40 molecules. We also studied the antigen presenting function of ginger extract treated macrophages by primary mixed lymphocyte reaction.ResultsWe observed that ginger extract inhibited IL-12, TNF-α, IL-1β (pro inflammatory cytokines) and RANTES, MCP-1 (pro inflammatory chemokines) production in LPS stimulated macrophages. Ginger extract also down regulated the expression of B7.1, B7.2 and MHC class II molecules. In addition ginger extract negatively affected the antigen presenting function of macrophages and we observed a significant reduction in T cell proliferation in response to allostimulation, when ginger extract treated macrophages were used as APCs. A significant decrease in IFN-γ and IL-2 production by T cells in response to allostimulation was also observed.ConclusionIn conclusion ginger extract inhibits macrophage activation and APC function and indirectly inhibits T cell activation.

[1]  Ha Won Kim,et al.  Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages. , 2005, Antioxidants & redox signaling.

[2]  R. Jack,et al.  Macrophages as main inducers of IFN-gamma in T cells following administration of human and mouse heat shock protein 60. , 2002, International immunology.

[3]  V. Appay,et al.  RANTES: a versatile and controversial chemokine. , 2001, Trends in immunology.

[4]  S. Kittur,et al.  In vitro immunomodulatory effects of ten commonly used herbs on murine lymphocytes. , 2002, Journal of alternative and complementary medicine.

[5]  R. Grzanna,et al.  Ginger extract inhibits beta-amyloid peptide-induced cytokine and chemokine expression in cultured THP-1 monocytes. , 2004, Journal of alternative and complementary medicine.

[6]  N. E. Miller,et al.  Regulation of macrophage activation , 2003, Cellular and Molecular Life Sciences CMLS.

[7]  K. Grant,et al.  Alternative therapies: ginger. , 2000 .

[8]  D. Mosser,et al.  The many faces of macrophage activation , 2003, Journal of leukocyte biology.

[9]  K. Maier,et al.  Effect of 6-gingerol on pro-inflammatory cytokine production and costimulatory molecule expression in murine peritoneal macrophages. , 2007, The Journal of surgical research.

[10]  An in vitro screening assay for inhibitors of proinflammatory mediators in herbal extracts using human synoviocyte cultures , 2007, In Vitro Cellular & Developmental Biology - Animal.

[11]  K. C. Srivastava,et al.  Ginger (Zingiber officinale) in rheumatism and musculoskeletal disorders. , 1992, Medical hypotheses.

[12]  D. Hungerford,et al.  Ginger extract components suppress induction of chemokine expression in human synoviocytes. , 2005, Journal of alternative and complementary medicine.

[13]  R. Margreiter,et al.  Heat shock protein expression in the transplanted human kidney , 2001, Transplant international : official journal of the European Society for Organ Transplantation.

[14]  S. Kittur,et al.  In Vitro Immunomodulatory Effects of Herbal Products , 2002, The American surgeon.

[15]  R. Atkins,et al.  The Role of Macrophages in Allograft Rejection , 2005, Transplantation.

[16]  B. Rollins.  Monocyte chemoattractant protein 1: a potential regulator of monocyte recruitment in inflammatory disease. , 1996, Molecular medicine today.