Nanoroughened plasmonic films for enhanced biosensing detection

Although fluorescence is the prevailing labeling technique in biosensing applications, sensitivity improvement is still a striving challenge. We show that coating standard microscope slides with nanoroughened silver films provides a high fluorescence signal enhancement due to plasmonic interactions. As a proof of concept, we applied these films with tailored plasmonic properties to DNA microarrays. Using common optical scanning devices, we achieved signal amplifications of more than 40-fold.

[1]  W. Barnes,et al.  Efficient coupling of surface plasmon polaritons to radiation using a bi-grating , 2001 .

[2]  C. Goutel,et al.  Double interference fluorescence enhancement from reflective slides: Application to bicolor microarrays , 2005 .

[3]  Jörg Enderlein,et al.  Single-molecule fluorescence near a metal layer , 1999 .

[5]  D. Loerke,et al.  Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release. , 1999, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[6]  Jeffrey N. Anker,et al.  Biosensing with plasmonic nanosensors. , 2008, Nature materials.

[7]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[8]  H. Raether.  Surface Plasmons on Smooth and Rough Surfaces and on Gratings , 1988 .

[9]  J M Delabar,et al.  Classification of human chromosome 21 gene-expression variations in Down syndrome: impact on disease phenotypes. , 2007, American journal of human genetics.

[10]  Oliver King,et al.  Directional, enhanced fluorescence from molecules near a periodic surface. , 1994, Applied optics.

[11]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[12]  Piers Andrew,et al.  Molecular fluorescence above metallic gratings , 2001 .

[13]  W. Barnes,et al.  Surface plasmon subwavelength optics , 2003, Nature.

[14]  E. Ozbay.  Plasmonics: Merging Photonics and Electronics at Nanoscale Dimensions , 2006, Science.

[15]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[16]  Su,et al.  Localization of the surface plasmon polariton caused by random roughness and its role in surface-enhanced optical phenomena. , 1985, Physical review letters.

[17]  Paul K. Hansma,et al.  Efficiency of light emission from surface plasmons , 1982 .

[18]  F. Aussenegg,et al.  Novel aspects of fluorescence lifetime for molecules positioned close to metal surfaces , 1987 .

[19]  Marie-Pierre Fontaine-Aupart,et al.  Enhanced Fluorescence Cell Imaging with Metal-Coated Slides , 2007 .

[20]  E. Fort,et al.  Surface enhanced fluorescence , 2008 .

[21]  Andrew G. Glen,et al.  APPL , 2001 .

[22]  S. Hell,et al.  Properties of a 4Pi confocal fluorescence microscope , 1992 .

[23]  Igor L. Medintz,et al.  Quantum dot bioconjugates for imaging, labelling and sensing , 2005, Nature materials.

[24]  Vladimir M. Shalaev,et al.  Electromagnetic field fluctuations and optical nonlinearities in metal-dielectric composites , 2000 .