Characteristics of a biofilm photobioreactor as applied to photo-hydrogen production.

The application of a cell immobilization technique to a biofilm-based photobioreactor was developed to enhance its photo-hydrogen production rate and light conversion efficiency. Rhodopseudomonas palustris CQK 01 was initially attached to the surface of packed glass beads to form a biofilm in this experiment. Then, the biofilm photobioreactor (BPBR) was illuminated by light-emitting diodes with light wavelengths of 470, 590 and 630 nm and hydrogen was evolved with glucose being the sole carbon source. Under the illumination condition of 5000 lux illumination intensity and 590 nm wavelength, the BPBR showed good hydrogen production performance: the hydrogen production rate was 38.9 ml/l/h and light conversion efficiency was 56%, while the hydrogen yield was 0.2 mol H(2)/ mol glucose. Furthermore, results show that the highest hydrogen production rate and glucose removal rate were obtained when the glucose concentration is 0.12 M, the optimal pH 7 and optimal temperature of influent liquid 25 degrees C.

[1]  P. Vignais,et al.  Hydrogen production by Rhodopseudomonas capsulata cells entrapped in carrageenan beads , 1984, Biotechnology Letters.

[2]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[3]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[4]  G. Kohring,et al.  Enhanced hydrogen production from aromatic acids by immobilized cells of Rhodopseudomonas palustris , 1995, Applied Microbiology and Biotechnology.

[5]  D. Madamwar,et al.  Prolonged evolution of photohydrogen by intermittent supply of nitrogen using a combined system of Phormidium valderianum, Halobacterium halobium, and Escherichia coli , 1998 .

[6]  Debabrata Das,et al.  Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. , 2001 .

[7]  J. Miyake,et al.  Efficiency of light energy conversion to hydrogen by the photosynthetic bacterium Rhodobacter sphaeroides , 1987 .

[8]  Hanqing Yu,et al.  Response surface analysis on the effect of cell concentration and light intensity on hydrogen production by Rhodopseudomonas capsulata , 2005 .

[9]  Jo-Shu Chang,et al.  Anaerobic hydrogen production with an efficient carrier‐induced granular sludge bed bioreactor , 2004, Biotechnology and bioengineering.

[10]  Jo-Shu Chang,et al.  Enhancing phototropic hydrogen production by solid-carrier assisted fermentation and internal optical-fiber illumination , 2006 .

[11]  A. Tsygankov,et al.  Immobilization of the purple non-sulfur bacterium Rhodobacter sphaeroides on glass surfaces , 1993 .

[12]  René H. Wijffels,et al.  Photobiological hydrogen production: photochemical e)ciency and bioreactor design , 2002 .

[13]  E Fascetti,et al.  Photosynthetic hydrogen evolution with volatile organic acids derived from the fermentation of source selected municipal solid wastes , 1998 .

[14]  E. Fascetti,et al.  Rhodobacter sphaeroides RV cultivation and hydrogen production in a one- and two-stage chemostat , 1995, Applied Microbiology and Biotechnology.

[15]  Basar Uyar,et al.  Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors , 2007 .

[16]  Mi-Sun Kim,et al.  Photoproduction of hydrogen from acetate by a chemoheterotrophic bacterium Rhodopseudomonas palustris P4 , 2004 .

[17]  Tatsuki Wakayama,et al.  Light shade bands for the improvement of solar hydrogen production efficiency by Rhodobacter sphaeroides RV , 2002 .

[18]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .

[19]  Jo-Shu Chang,et al.  Fermentative hydrogen production and bacterial community structure in high‐rate anaerobic bioreactors containing silicone‐immobilized and self‐flocculated sludge , 2006, Biotechnology and bioengineering.

[20]  R. Banerjee,et al.  Comparison of biohydrogen production processes , 2008 .

[21]  Chiu-Yue Lin,et al.  Biohydrogen production using an up-flow anaerobic sludge blanket reactor , 2004 .

[22]  I. Eroglu,et al.  Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides , 2002 .

[23]  Jo‐Shu Chang,et al.  Hydrogen production by indigenous photosynthetic bacterium Rhodopseudomonas palustris WP3–5 using optical fiber-illuminating photobioreactors , 2006 .

[24]  T. Wakayama,et al.  Entrapment of Rhodobacter sphaeroides RV in cationic polymer/agar gels for hydrogen production in the presence of NH4+. , 1999, Journal of bioscience and bioengineering.

[25]  Joo-Hwa Tay,et al.  Biohydrogen production with anaerobic fluidized bed reactors—A comparison of biofilm-based and granule-based systems , 2008 .

[26]  Mi-Sun Kim,et al.  Thermophilic biohydrogen production from glucose with trickling biofilter. , 2004, Biotechnology and bioengineering.

[27]  Tong Zhang,et al.  Characteristics of a phototrophic sludge producing hydrogen from acetate and butyrate , 2008 .

[28]  K. Sasikala,et al.  Environmental regulation for optimal biomass yield and photoproduction of hydrogen by Rhodobacter sphaeroides O.U. 001 , 1991 .

[29]  Wei Liu,et al.  Photo-hydrogen production rate of a PVA-boric acid gel granule containing immobilized photosynthetic bacteria cells , 2009 .

[30]  Tong Zhang,et al.  Phototrophic hydrogen production from acetate and butyrate in wastewater , 2005 .

[31]  Sung Ho Yeom,et al.  Immobilization methods for continuous hydrogen gas production biofilm formation versus granulation , 2005 .

[32]  Jo‐Shu Chang,et al.  Feasibility study on bioreactor strategies for enhanced photohydrogen production from Rhodopseudomonas palustris WP3-5 using optical-fiber-assisted illumination systems , 2006 .

[33]  J. Tramper,et al.  Acetate as a carbon source for hydrogen production by photosynthetic bacteria. , 2001, Journal of biotechnology.

[34]  Chun-Chin Wang,et al.  Photohydrogen production using purple nonsulfur bacteria with hydrogen fermentation reactor effluent , 2002 .

[35]  C. Ramana,et al.  Regulation of simultaneous hydrogen photoproduction during growth by pH and glutamate in Rhodobacter sphaeroides O.U. 001 , 1995 .

[36]  Kadir Aslan,et al.  Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor , 1999 .

[37]  A. Tsygankov,et al.  Photobioreactor with photosynthetic bacteria immobilized on porous glass for hydrogen photoproduction , 1994 .

[38]  The photosynthetic apparatus of Rhodobacter sphaeroides. , 1999, Trends in microbiology.