Studies on plasmids of multidrug resistant uropathogens isolated from patients with urinary tract infection in a tertiary hospital in Calabar, Nigeria

Background Urinary tract Infections caused by multidrug resistant uropathogens have become a significant global public health problem with Nigeria being no exception. Objective This study is aimed at profiling and curing the plasmids of selected multidrug resistant uropathogens isolated from patients with urinary tract infection in a tertiary hospital in Calabar, Nigeria. Methodology Isolates were obtained from urine samples of patients using standard microbiological techniques. Multidrug resistant bacterial isolates were then selected for plasmid DNA analysis and curing. Results The study revealed that E. coli, K. pneumoniae, P. aeruginosa and Proteus mirabilis were resistant to the antibiotics tested. The extracted plasmid DNA showed the presence of TEM, SHV and CTX-M genes in the isolates with sizes of 400-600bp, 300bp and 500-800bp, respectively. All isolates possessed the SHV genes while few had TEM and CTX-M genes. Cells were subjected to curing and plasmid curing was achieved at 200-300µl of ethidium bromide. Conclusion The reduction in percentage resistance due to plasmid curing observed in this study suggests that the resistance of the isolates to antibiotics were plasmid-mediated. Antibiogram and monitoring of plasmid mediated resistance are necessary for proper management of urinary tract infections.

[1]  A. Jaran.  Antimicrobial Resistance Patterns and Plasmid Profiles of Staphylococcus Aureus Isolated from Different Clinical Specimens in Saudi Arabia , 2017 .

[2]  L. Arsalane,et al.  Antimicrobial susceptibility of urinary Klebsiella pneumoniae and the emergence of carbapenem-resistant strains: A retrospective study from a university hospital in Morocco, North Africa , 2015 .

[3]  P. Akinduti,et al.  Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria. , 2015, African health sciences.

[4]  A. Weintraub,et al.  Antibiotic resistance patterns of Escherichia coli isolates from different aquatic environmental sources in León, Nicaragua. , 2012, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[5]  Prof. M.R Shakibaie,et al.  Molecular Identification of ESBL Genesbla GES-1, blaVEB-1, blaCTX-M blaOXA-1, blaOXA-4,blaOXA-10 and blaPER-1 in Pseudomonas aeruginosa Strains Isolated from Burn Patientsby PCR, RFLP and Sequencing Techniques , 2010 .

[6]  M. Altındiş,et al.  Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals. , 2007, Indian journal of medical microbiology.

[7]  J. Campos,et al.  Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions , 2006, Journal of Clinical Microbiology.

[8]  L. Amaral,et al.  The mechanism of plasmid curing in bacteria. , 2006, Current drug targets.

[9]  Christopher M Thomas,et al.  Mechanisms of, and Barriers to, Horizontal Gene Transfer between Bacteria , 2005, Nature Reviews Microbiology.

[10]  H. Shah,et al.  Microbiological profile of urinary tract infection in a tertiary care hospital , 2016 .

[11]  A. Onilude,et al.  Plasmid Curing and Its Effect on the Growth and Physiological Characteristics of Lactobacillus plantarum Isolated from Fermented Cereals , 2015 .

[12]  Mogahid M. Elhassan,et al.  Prevalence of TEM, SHV and CTX-M genes in Escherichia coli and Klebsiella spp Urinary Isolates from Sudan with confirmed ESBL phenotype , 2013 .

[13]  Ayad M.A. Fadhil اياد محمد علي فاضل,et al.  Detection of plasmid DNA profile in bacterial and fungal isolates from catheterized patients and its relation with antibiotic susceptibility , 2013 .

[14]  C. Rohde,et al.  PLASMID ISOLATION FROM BACTERIA Some fast step-by-step procedures tried out at the DSMZ , 2011 .

[15]  M. Ferraro.  Performance standards for antimicrobial susceptibility testing , 2001 .

[16]  S. Amyes,et al.  Antibiotic resistance in bacteria. , 1992 .