Studies of the Cell Population Kinetics of Induced and Transplanted Mammary Adenocarcinoma in Rats

The length of the cell cycle and the thymidine index (less than 1 day and 6.5%, respectively) for growing, 7,12-dimethylbenz(a)anthracene-induced mammary adenocarcinoma in female Sprague-Dawley rats vary only slightly over a 15-fold change in tumor mass. The observed decreasing growth rate with increasing tumor mass is attributed to a change in the balance of cell production to cell loss. The induced tumor, regressed by ovariectomy and stimulated by estradiol and progesterone, has a shorter doubling time, a 1-day cell cycle time, and a 11% thymidine index. The proliferative fraction of hormone-responsive tumors is modified by hormonal intervention. A transplanted mammary adenocarcinoma, derived from a 7,12-dimethylbenz(a)anthracene-induced tumor in Fischer rats, has the shortest doubling time, a slightly shorter cell cycle time, and the highest thymidine index (15%). Computer analysis by two mathematical models for the percentage labeled mitoses data is presented for comparison.

[1]  G H Weiss,et al.  Numerical inversion of Laplace transforms with application to percentage labeled mitoses experiments. , 1972, Computers and biomedical research, an international journal.

[2]  H. R. Lindner,et al.  Kinetics of DNA synthesis in immature rat uterus: age dependence and estradiol stimulation. , 1972, Biochimica et biophysica acta.

[3]  G. Steel,et al.  THE TECHNIQUE OF LABELLED MITOSES: ANALYSIS BY AUTOMATIC CURVE‐FITTING , 1971, Cell and tissue kinetics.

[4]  H. H. Lloyd,et al.  Kinetic parameters and growth curves for experimental tumor systems. , 1970, Cancer chemotherapy reports.

[5]  R. King,et al.  The effect of dimethylbenzanthracene on the incorporation of [3H] thymidine into DNA of rat mammary gland and uterus. , 1970, European Journal of Cancer.

[6]  D. Griswold,et al.  Observations on the hormone sensitivity of 7,12-dimethylbenz(alpha)anthracene-induced mammary tumors in the Sprague-Dawley rat. , 1970, Cancer research.

[7]  D. Griswold,et al.  Studies of the kinetics of growth and regression of 7,12-dimethylbenz(alpha)anthracene-induced mammary adenocarcinoma in Sprague-Dawley rats. , 1970, Cancer research.

[8]  Laird Ak,et al.  Dynamics of growth in tumors and in normal organisms. , 1969 .

[9]  N B Schwartz,et al.  A model for the regulation of ovulation in the rat. , 1969, Recent progress in hormone research.

[10]  P. Galand,et al.  Radioautographic evaluation of the estrogen-dependent proliferative pool in the stem cell compartment of the mouse uterine and vaginal epithelia. , 1967, Experimental cell research.

[11]  P. Daniel,et al.  Further studies on mammary tumours induced in rats by 7,12‐dimethylbenz(a)anthracene (DMBA) , 1967, International journal of cancer.

[12]  J. C. Barrett.  A mathematical model of the mitotic cycle and its application to the interpretation of percentage labeled mitoses data. , 1966, Journal of the National Cancer Institute.

[13]  H. Skipper,et al.  Induced mammary carcinoma in the female rat as a drug evaluation system. , 1966, Cancer research.

[14]  F. Bresciani.  EFFECT OF OVARIAN HORMONES ON DURATION OF DNA SYNTHESIS IN CELLS OF THE C3H MOUSE MAMMARY GLAND. , 1965, Experimental cell research.

[15]  B. Peckham,et al.  AUTORADIOGRAPHIC INVESTIGATION OF ESTROGEN RESPONSE MECHANISMS IN RAT VAGINAL EPITHELIUM. , 1963, American journal of obstetrics and gynecology.

[16]  C. Huggins,et al.  Mammary Cancer Induced by a Single Feeding of Polynuclear Hydrocarbons, and its Suppression , 1961, Nature.

[17]  M L Mendelsohn,et al.  The growth fraction, a new concept applied to tumors. Abstr. , 1960 .

[18]  H. Sutton,et al.  RAPID INDUCTION OF MAMMARY CARCINOMA IN THE RAT AND THE INFLUENCE OF HORMONES ON THE TUMORS , 1959, Journal of Experimental Medicine.