Relationships of the Col plasmids E2, E3, E4, E5, E6, and E7: restriction mapping and colicin gene fusions.

Thirteen ColE plasmids representing the E2-E7 types have been compared by restriction mapping. Over 80% of their restriction sites were found to be similarly positioned, indicating that these plasmids share a common structure. Three variants are ColE2-CA42 and ColE7-K317, both of which contain 1.8-kb DNA segments in place of a 2.5-kb segment common to the other plasmids, and ColE6-CT14, which has an additional 5.0-kb DNA segment compared to the other plasmids. The colicin (col), immunity (imm), and colicin release (hic) genes of these plasmids have been localized to regions corresponding to those known for ColE3-CA38 and ColE2-P9, with the imm and hic genes adjacent to the 3' end of the col gene. Active colicin is produced from hybrid col genes containing 5' and 3' ends from different E-type plasmids. The 3'-termini of the fused col genes specify the colicin type.

[1]  R. James,et al.  Localization and characterization of a gene on the ColE3-CA38 plasmid that confers immunity to colicin E8. , 1984, Journal of general microbiology.

[2]  P. Reeves.  Mode of Action of Colicins of Types E1, E2, E3, and K , 1968, Journal of bacteriology.

[3]  A. Pugsley,et al.  The BtuB Group Col Plasmids and Homology Between the Colicins They Encode , 1982, Journal of bacteriology.

[4]  M. Nomura,et al.  Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells. , 1971, Nature: New biology.

[5]  R. Ben-Gurion,et al.  Mode of Action of Pesticin , 1969, Journal of bacteriology.

[6]  R. Watson,et al.  Identification and characterization of Col plasmids from classical colicin E-producing strains , 1981, Journal of bacteriology.

[7]  R. James,et al.  Two new E colicins, E8 and E9, produced by a strain of Escherichia coli. , 1984, Journal of general microbiology.

[8]  R. Watson,et al.  The immunity genes of colicins E2 and E8 are closely related , 1984, Bioscience reports.

[9]  T. Vernet,et al.  Characterization and nucleotide sequence of a colicin-release gene in the hic region of plasmid ColE3-CA38. , 1984, Gene.

[10]  H. Masaki,et al.  A plasmid region encoding the active fragment and the inhibitor protein of colicin E3—CA38 , 1982, FEBS letters.

[11]  J. Inselburg,et al.  Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites , 1975, Journal of bacteriology.

[12]  K. Imahori,et al.  Assignment of the functional loci in colicin E2 and E3 molecules by the characterization of their proteolytic fragments. , 1980, Biochemistry.

[13]  Cloning of the ColE3-CA38 colicin and immunity genes and identification of a plasmid region which enhances colicin production. , 1982, Gene.

[14]  R. Watson,et al.  Restriction endonuclease mapping of ColE2-P9 and ColE3-CA38 plasmids. , 1980, Gene.

[15]  B. Stocker,et al.  Colicins E4, E5, E6 and A and properties of btuB+ colicinogenic transconjugants. , 1982, Journal of general microbiology.