An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species.

With the implementation of programs to control lymphatic filariasis and soil-transmitted helminths using broad spectrum anthelmintics, including albendazole and ivermectin, there is a need to develop an in vitro assay for detection of drug resistance. This report describes an in vitro assay for measuring the effects of ivermectin and benzimidazoles on the motility of larvae of the hookworm species Ancylostoma ceylanicum, A. caninum, and Necator americanus, and Strongyloides species including Strongyloides stercoralis, and S. ratti. A dose-response relationship was demonstrated with each of the parasite species, with distinct differences observed between the various species. In pilot field testing of the assay with N. americanus larvae recovered from human fecal samples, a dose-response relationship was observed with ivermectin. While the assay has demonstrated the ability to determine drug responsiveness, its usefulness in resistance detection will require correlation with the clinical outcome among individuals infected with parasite strains showing different drug sensitivities.

[1]  V. Wright,et al.  Molecular analysis of the beta-tubulin gene of human hookworms as a basis for possible benzimidazole resistance on Pemba Island. , 2004, Molecular and biochemical parasitology.

[2]  M. Taylor,et al.  Anthelmintic resistance detection methods. , 2002, Veterinary parasitology.

[3]  J. Habbema,et al.  Can ivermectin mass treatments eliminate onchocerciasis in Africa? , 2002, Bulletin of the World Health Organization.

[4]  T. Nikaido,et al.  Nematocidal activities of thiabendazole and ivermectin against the larvae of Strongyloides ratti and S. venezuelensis. , 2001, Veterinary parasitology.

[5]  D. Pritchard,et al.  Predisposition to hookworm infection in Papua New Guinea. , 2001, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[6]  B Gryseels,et al.  Drug resistance in human helminths: current situation and lessons from livestock. , 2000, Clinical microbiology reviews.

[7]  M. Várady,et al.  Comparison of six in vitro tests in determining benzimidazole and levamisole resistance in Haemonchus contortus and Ostertagia circumcincta of sheep. , 1999, Veterinary parasitology.

[8]  J. Humbert,et al.  PCR diagnosis of benzimidazole-susceptibility or -resistance in natural populations of the small ruminant parasite, Teladorsagia circumcincta. , 1999, Veterinary parasitology.

[9]  N. Sangster.  Anthelmintic resistance: past, present and future. , 1999, International journal for parasitology.

[10]  J. Williams,et al.  Diagnostic medical parasitology. , 1998, Parasitology today.

[11]  F. Gilbert,et al.  Failure of pyrantel in treatment of human hookworm infections (Ancylostoma duodenale) in the Kimberley region of north west Australia. , 1997, Acta tropica.

[12]  J. Vercruysse,et al.  Failure of mebendazole in treatment of human hookworm infections in the southern region of Mali. , 1997, The American journal of tropical medicine and hygiene.

[13]  J. Richards,et al.  In vitro studies on the relative sensitivity to ivermectin of Necator americanus and Ancylostoma ceylanicum. , 1995, International journal for parasitology.

[14]  D. Simeon,et al.  Treatment of Trichuris trichiura infections improves growth, spelling scores and school attendance in some children. , 1995, The Journal of nutrition.

[15]  M. Kwa,et al.  Benzimidazole resistance in Haemonchus contortus is correlated with a conserved mutation at amino acid 200 in β-tubulin isotype 1 , 1994 .

[16]  R. Prichard.  Anthelmintic resistance. , 1994, Veterinary parasitology.

[17]  J. Behnke,et al.  Sensitivity to ivermectin and pyrantel of Ancylostoma ceylanicum and Necator americanus. , 1993, International journal for parasitology.

[18]  D. Bundy,et al.  Moderate to heavy infections of Trichuris trichiura affect cognitive function in Jamaican school children , 1992, Parasitology.

[19]  B. E. Matthews,et al.  On the biological and biochemical nature of cloned populations of Strongyloides ratti , 1992, Journal of Helminthology.

[20]  E. Lacey,et al.  Detection of resistance to ivermectin in Haemonchus contortus. , 1991, International journal for parasitology.

[21]  C. Schofield,et al.  UNDP/World Bank/WHO special programme for research and training in tropical diseases (TDR) , 1989 .

[22]  J. Behnke,et al.  Ancylostoma ceylanicum in the hamster: observations on the host—parasite relationship during primary infection , 1989, Parasitology.

[23]  J. Behnke,et al.  An improved technique for experimental infections with skin penetrating nematode larvae (Necator americanus). , 1986, International journal for parasitology.

[24]  R. Jarrett,et al.  Resistance to benzimidazole anthelmintics in field strains of Ostertagia and Nematodirus in sheep. , 1985, Australian veterinary journal.

[25]  Enzie Fd,et al.  Cross-resistance to other anthelmintics in an experimentally produced cambendazole-resistant strain of Haemonchus contortus in lambs. , 1975 .

[26]  K. C. Kates,et al.  Cross-resistance to other anthelmintics in an experimentally produced cambendazole-resistant strain of Haemonchus contortus in lambs. , 1975, The Journal of parasitology.