论文信息 - Nutrient requirements of suspension cultures of soybean root cells.

Nutrient requirements of suspension cultures of soybean root cells.

Abstract The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2,4- d ) has been designed. The cells required thiamine, 2,4- d and ammonium in addition to the usual mineral salts and sucrose. Optimum concentrations of nitrate and ammonium were 25 and 2 m M respectively. The highest yield of cells was achieved at an initial pH of 4.5–5.5. During the growth cycle the pH gradually increased to 6.0–6.2.

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