In vitro sensitivity pattern of some urinary tract isolates to Carica papaya extracts

Powdered leaves of Carica papaya (L.) were extracted with ethanol and partitioned in chloroform and distilled water. The extract and fractions were tested for antibacterial activity against clinical isolates of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas species using disc diffusion and microbroth dilution technique. The extract and fractions were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of sensitivity test results showed that ethanol extract of the leaf was active against E. coli and K. pneumoniae (7mm each) at 1000μg/disc concentration while chloroform and water fractions of the leaf were active against Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis at 1000μg/disc concentration with zone diameter of 7mm each. Both the extract and fractions were inactive against P. aeruginosa at all concentrations used in this study. The results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids and tannins in either ethanol extract, fraction(s) or both. This indicates that the Carica papaya has the potential for the production of drugs against organisms causing urinary tract infections. Keywords: Sensitivity, Clinical isolates, Urinary tract, Carica papaya , Extract, Fractions