Continuous production of an antibiotic polypeptide (nisin) by Lactococcus lactis using a bioreactor coupled to a microfiltration module

The continuous production of nisin, an antibiotic polypeptide, by Lactococcus lactis in a bioreactor system coupled to a microfiltration module is described. Nisin productivity with respect to both cultivation time (ND) and the quantity of glucose consumed (ND/Sf) in continuous production was enhanced by maintaining a low concentration of lactic acid in the broth. A maximum ND of 7.80 × 104 U·l−1·h−1 and ND/Sf of 5.20 × 103 U·g−1·h−1 were obtained when the glucose concentration in the feed medium was 15 g/l. These values represent about 4.1- and 4.5-fold increases, respectively, over those obtained in batch culture.

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