This paper studies the effects of Lobelia chinensis on colon precancerous lesions and on colonic epithelial proliferation and apoptosis in DMH-induced rats. After two weeks of feeding, 50 Wistar rats were randomly divided into five groups, namely the normal group, model group, Lobelia chinensis low-dose group, medium-dose group and high-dose group. Lobelia chinensis was made into ACF model, and administered to experimental groups for 10 consecutive weeks. Control group was given equivalent amount of normal saline. After feeding for 10 weeks, the rats in each group were sacrificed and the changes in colonic ACF number of rats in experimental groups were observed, and the inhibition rates were calculated. The results showed that among the rats fed for 24 h and 48 h, the number of apoptotic cells in colonic crypts of rats in DMH group did not differ significantly from the control group, while the difference was obvious between the control group and Lobelia chinensis treatment groups. The medium and high doses, that is, 0.45 g/kg and 1.35 g/kg can significantly inhibit ACF formation (P<0.01). The inhibition rates of low, medium and high doses were 8.12%, 59.42% and 65.44%, respectively.
[1]
N. Takai,et al.
Bufalin, a traditional oriental medicine, induces apoptosis in human cancer cells.
,
2012,
Asian Pacific journal of cancer prevention : APJCP.
[2]
C. Cho,et al.
Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells.
,
2008,
Journal of ethnopharmacology.
[3]
Wu Guo-xin.
Extract of alkaloids from Lobelia chinensis Lour and their inhibitory effects on growth of Myeloma Cells U266
,
2012
.
[4]
Yang Jun.
Extraction of Alkaloids from Lobelia chinensis Lour and Their Inhibitory Effects on Growth of Stomach Cancer Cells
,
2007
.