Antiurolithiatic activity of Pinus eldarica medw: fruits aqueous extract in rats.

PURPOSE To evaluate the antiurolithiatic activity of Pinus eldarica fruit on induced calcium oxalate nephrolithiasis in rats. MATERIALS AND METHODS Calcium oxalate nephrolithiasis in rats was induced by administering ethylene glycol 1% for 30 days via drinking water. The prophylactic and therapeutic groups received P. eldarica fruit extract (500 and 1000 mg/kg/day) as well for 30 days and from the 14th day through the end of the experiment, respectively. The following variables were assessed; urine volume, urinary calcium excretion, and crystalluria. Finally, rats' kidneys were histopathologically examined. RESULTS The aqueous extract prophylactic treatment (500 mg/kg/day) increased urinary calcium excretion. Qualitative analysis of crystalluria and histopathologic examination showed that the administered dose of extract prevented stone formation in the kidneys significantly. The prophylactic treatment did not increase urine volume in comparison with ethylene glycol. Stone formation did not decrease in the treatment group. CONCLUSION This study indicates that P. eldarica fruit extract prevents calcium oxalate deposition, without producing diuresis.

[1]  M. Hadjzadeh,et al.  Effect of thymoquinone on ethylene glycol-induced kidney calculi in rats. , 2008, Urology journal.

[2]  S. P. Preetha,et al.  Beneficial role of sulfated polysaccharides from edible seaweed Fucus vesiculosus in experimental hyperoxaluria , 2007 .

[3]  Bardaoui Mourad,et al.  Influence of hypercalcic and/or hyperoxalic diet on calcium oxalate renal stone formation in rats , 2006 .

[4]  J. Busserolles,et al.  In vivo antioxidant activity of procyanidin-rich extracts from grape seed and pine (Pinus maritima) bark in rats. , 2006, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[5]  F. Neffati,et al.  Influence of hypercalcic and/or hyperoxalic diet on calcium oxalate renal stone formation in rats. , 2006, Scandinavian journal of urology and nephrology.

[6]  N. Murugesh,et al.  Antilithiatic effect of Asparagus racemosus Willd on ethylene glycol-induced lithiasis in male albino Wistar rats. , 2005, Methods and findings in experimental and clinical pharmacology.

[7]  M. Menon,et al.  Vitamin E therapy prevents hyperoxaluria‐induced calcium oxalate crystal deposition in the kidney by improving renal tissue antioxidant status , 2005, BJU international.

[8]  S. Afsharypuor,et al.  Essential Oil Constituents of Leaves and Fruits of Pinus eldarica Medw , 2005 .

[9]  R. Perez-Fernandez,et al.  In‐vitro anti‐inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts: effect on inducible NOS, COX‐1, COX‐2 and their products in J774A.1 murine macrophages , 2005, The Journal of pharmacy and pharmacology.

[10]  K. Tozawa,et al.  Preventive effects of green tea on renal stone formation and the role of oxidative stress in nephrolithiasis. , 2005, The Journal of urology.

[11]  H. Chung,et al.  Antioxidant principles from the needles of red pine, Pinus densiflora , 2003, Phytotherapy Research.

[12]  R. Dean,et al.  Isolation of a novel antimicrobial peptide gene (Sp-AMP) homologue from Pinus sylvestris (Scots pine) following infection with the root rot fungus Heterobasidion annosum. , 2003, FEMS microbiology letters.

[13]  N. Çíftçíoglu,et al.  Characteristics of nanobacteria and their possible role in stone formation , 2003, Urological Research.

[14]  M. Dığrak,et al.  Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra , 1999, Phytotherapy research : PTR.

[15]  G. Rimbach,et al.  Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. , 1999, Free radical biology & medicine.

[16]  M. Friedman.  Nonsteroidal anti-inflammatory drugs facilitate stone passage. , 1990, Urology.

[17]  H. Sakagami,et al.  Antimicrobial spectrum of lignin-related pine cone extracts of Pinus parviflora Sieb. et Zucc. , 1990, In vivo.

[18]  H. Sakagami,et al.  Induction of antimicrobial activity by antitumor substances from pine cone extract of Pinus parviflora Sieb. et Zucc. , 1988, Anticancer research.

[19]  R. Börner,et al.  Clinical results in the treatment of therapy-resistant calcium-stone formers with non-steroidal anti-inflammatory drugs. , 1987, European urology.