Coordinate accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defense response during fruit ripening in grape.

During ripening of grape (Vitis labruscana L. cv Concord) berries, abundance of several proteins increased, coordinately with hexoses, to the extent that these became the predominant proteins in the ovary. These proteins have been identified by N-terminal amino acid-sequence analysis and/or function to be a thaumatin-like protein (grape osmotin), a lipid-transfer protein, and a basic and an acidic chitinase. The basic chitinase and grape osmotin exhibited activities against the principal grape fungal pathogens Guignardia bidwellii and Botrytis cinerea based on in vitro growth assays. The growth-inhibiting activity of the antifungal proteins was substantial at levels comparable to those that accumulate in the ripening fruit, and these activities were enhanced by as much as 70% in the presence of 1 m glucose, a physiological hexose concentration in berries. The simultaneous accumulation of the antifungal proteins and sugars during berry ripening was correlated with the characteristic development of pathogen resistance that occurs in fruits during ripening. Taken together, accumulation of these proteins, in combination with sugars, appears to constitute a novel, developmentally regulated defense mechanism against phytopathogens in the maturing fruit.

[1]  P. Høj,et al.  Identification and Characterization of a Fruit-Specific, Thaumatin-Like Protein That Accumulates at Very High Levels in Conjunction with the Onset of Sugar Accumulation and Berry Softening in Grapes , 1997, Plant physiology.

[2]  I. Dry,et al.  A Class IV Chitinase Is Highly Expressed in Grape Berries during Ripening , 1997, Plant physiology.

[3]  U. Sonnewald,et al.  Salicylic acid‐independent induction of pathogenesis‐related protein transcripts by sugars is dependent on leaf developmental stage , 1996, FEBS letters.

[4]  J. Pozueta-Romero,et al.  Fruit-Specific Expression of a Defensin-Type Gene Family in Bell Pepper (Upregulation during Ripening and upon Wounding) , 1996, Plant physiology.

[5]  P. Hasegawa,et al.  Novel Osmotically Induced Antifungal Chitinases and Bacterial Expression of an Active Recombinant Isoform , 1996, Plant physiology.

[6]  P. Hasegawa,et al.  Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization , 1996 .

[7]  R. Bressan,et al.  Programmed accumulation of LEA-like proteins during desiccation and cold acclimation of overwintering grape buds , 1996 .

[8]  P. Sautière,et al.  A Cherry Protein and Its Gene, Abundantly Expressed in Ripening Fruit, Have Been Identified as Thaumatin-Like , 1996, Plant physiology.

[9]  W. Frommer,et al.  Systemic Acquired Resistance Mediated by the Ectopic Expression of Invertase: Possible Hexose Sensing in the Secretory Pathway. , 1996, The Plant cell.

[10]  P. Hasegawa,et al.  An Insecticidal N-Acetylglucosamine-Specific Lectin Gene from Griffonia simplicifolia (Leguminosae) , 1996, Plant physiology.

[11]  Luis González-Candelas,et al.  Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris , 1995, Journal of bacteriology.

[12]  J. Sheen,et al.  Sugar sensing in higher plants. , 1994, The Plant cell.

[13]  M. Flaishman,et al.  Timing of fungal invasion using host's ripening hormone as a signal. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[14]  B. Reisch,et al.  Wine and Juice Grape Varieties for Cool Climates , 1993 .

[15]  O. Maes,et al.  Four 9-kDa proteins excreted by somatic embryos of grapevine are isoforms of lipid-transfer proteins. , 1993, European journal of biochemistry.

[16]  M. Stitt,et al.  Regulation of the Expression of Rbcs and Other Photosynthetic Genes by Carbohydrates - a Mechanism for the Sink Regulation of Photosynthesis , 1993 .

[17]  S. Potter,et al.  Acquired resistance in Arabidopsis. , 1992, The Plant cell.

[18]  D. E. Nelson,et al.  Regulation of the Osmotin Gene Promoter. , 1992, The Plant cell.

[19]  Chi-Lien Cheng,et al.  Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[20]  S. Naito,et al.  Sugar-Dependent Expression of the CHS-A Gene for Chalcone Synthase from Petunia in Transgenic Arabidopsis. , 1991, Plant physiology.

[21]  W. K. Roberts,et al.  A new family of plant antifungal proteins. , 1991, Molecular plant-microbe interactions : MPMI.

[22]  J. Sheen,et al.  Metabolic repression of transcription in higher plants. , 1990, The Plant cell.

[23]  W. Peacock,et al.  Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. , 1990, The Plant cell.

[24]  R. Fluhr,et al.  Pathogenesis-related proteins are developmentally regulated in tobacco flowers. , 1989, The Plant cell.

[25]  P. Hasegawa,et al.  Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. , 1985, Plant physiology.

[26]  T. Arakawa,et al.  Stabilization of protein structure by sugars. , 1982, Biochemistry.

[27]  J. Lee,et al.  The stabilization of proteins by sucrose. , 1981, The Journal of biological chemistry.

[28]  J. Horsfall Fungi and fungicides the story of a nonconformist. , 1975, Annual review of phytopathology.

[29]  H. van der Wel,et al.  Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. , 1972, European journal of biochemistry.

[30]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[31]  김경희 수목의 흰가루병(Powdery mildew) , 2000 .

[32]  H. Ronne Glucose repression in fungi. , 1995, Trends in genetics : TIG.

[33]  B. Zoecklein Wine Analysis and Production , 1995 .

[34]  W. Pierpoint The major proteins in extracts of tobacco leaves that are responding hypersensitively to virus-infection , 1983 .

[35]  C. Dennis Post-harvest pathology of fruits and vegetables. , 1983 .

[36]  C. J. Delp Effect of temperature and humidity on the Grape powdery mildew fungus. , 1954 .