FTIR of touch imprint cytology: a novel tissue diagnostic technique.

Fourier transform infrared spectroscopic (FTIR) interrogation of biological tissues in real time has largely been a challenging proposition because of the strong absorption of mid-infrared light in water filled tissues. To enable sampling of tissues they must be sectioned and dried, which has time and resource implications. FTIR of touch imprint cytology (TIC) has been proposed to circumvent this problem. TIC is a well known histopathological method of rapidly analysing biological tissues. In this article we demonstrate the ability of FTIR of TIC to provide detailed spectra which can be used to differentiate various tissue pathologies. FTIR spectral profiles of TIC of lymph node and thyroid tissues differ visually when compared with TIC spectra of parathyroid tissue. The lymph node showed strong lipid spectral peaks at 1166cm(-1) and 1380cm(-1) including a very strong carbonyl-ester band at 1748cm(-1), and a strong methylene bending band (scissoring, at 1464cm(-1)). Smaller intensity protein peaks at 1547cm(-1) and 1659cm(-1) were also seen. The thyroid spectra, in addition to evident strong protein peaks at 1547cm(-1) and 1659cm(-1), also demonstrated possible nucleic acid signals at 1079cm(-1) and 1244cm(-1). The C-OH peak at 1037cm(-1) was attributed to carbohydrate signals. Parathyroid adenoma showed a marginal shift to lower wavenumbers with decreased amide I and II peak intensities when compared to hyperplasia. Nucleic acid peak positions at 1079cm(-1) and 1244cm(-1) were of higher intensity in adenomas compared to hyperplastic glands possibly demonstrating an increase in cell proliferation and growth. This study demonstrates the feasibility of cytoimprint FTIR for the intraoperative diagnosis of tissue during surgical neck exploration for the management of hyperparathyroidism. There is potential for the application of the technique in sentinel lymph node biopsy diagnosis and tumour margin evaluation.

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