论文信息 - Freeze-preservation of Leucocytozoon simondi sporozoites in liquid nitrogen.

Freeze-preservation of Leucocytozoon simondi sporozoites in liquid nitrogen.

SYNOPSIS. Sporozoites of L. simondi were maintained in a viable state for 7 months in liquid nitrogen. Comparison of parasite development initiated with fresh and frozen sporozoites showed a delay in development of each stage studied. Comparisons of prepatency, first elongate gametocytes, peak density of round and elongate forms, anemia and disappearance of megaloschizonts were made. In each phase there was a delay of 2–3 days in ducks infected with frozen sporozoites.

N. Kelker | R. Kocan | D. T. Clark

[1] C. B. Evans,et al. Fluorescent antibody studies on the immune response in sporozoite-induced and blood-induced vivax malaria and the relationship of antibody production to parasitemia. , 1966, The American journal of tropical medicine and hygiene.

[2] R. Kocan,et al. Anemia in ducks infected with Leucocytozoon simondi. , 1966, The Journal of protozoology.

[3] G. Jeffery. Survival of trophozoites of Plasmodium berghel and Plasmodium gallinaceum in glycerolized whole blood at low temperatures. , 1962, The Journal of parasitology.

[4] G. Jeffery. Extended low-temperature preservation of human malaria parasites. , 1957, The Journal of parasitology.

[5] R. C. Rendtorff,et al. Preservation of viable human malaria sporozoites by low-temperature freezing. , 1955, Experimental parasitology.

[6] D. M. Davies,et al. Life History of Leucocytozoon simondi Mathis and Leger in Natural and Experimental Infections and Blood Changes produced in the Avian Host. , 1951 .