Expression of hepatocyte nuclear factor 4α in primary ovarian mucinous tumors

Hepatocyte nuclear factor 4α (HNF4α) is a member of the nuclear receptor superfamily and is expressed in several endodermal tissues. The aim of the present study was to examine the expression of HNF4α on ovarian epithelial tumors with immunocytochemistry and immunohistochemistry using mAbs recognizing P1 and P2 promoter‐driven HNF4α. Ovarian mucinous adenoma, mucinous tumors of borderline malignancy, and mucinous adenocarcinoma had positive nuclear staining for HNF4α (41/45, 91%). One‐third (34%) of mucinous tumors had P1‐positive staining and most had P1/P2‐positive staining (93%). MUC2‐ and MUC5AC‐positive staining was observed in 34% and 95% of mucinous tumors, respectively. The histological subtype of these mucinous tumors was not correlated with HNF4α expression. On cytology it was found that cancer cells in the ascites from ovarian mucinous adenocarcinomas were HNF4α positive, but tumor cells in ascites from other types of ovarian carcinomas were negative for HNF4α. Thus, HNF4α is demonstrated to be a useful marker for histological and cytological diagnosis of ovarian mucinous tumors.

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